基于过氧化氢酶介导金纳米颗粒交联聚集的乙肝表面抗原可视化检测

doi:10.11896/cldb.21020098

材料导报

2022, Vol. 36

Issue (5): 21020098-4 https://doi.org/10.11896/cldb.21020098

金属与金属基复合材料

基于过氧化氢酶介导金纳米颗粒交联聚集的乙肝表面抗原可视化检测

陈如冰¹, 胡永琴^1,2, 陈美珠¹, 安佳^1,2, 吕颖¹, 刘玉菲^1,2, 李东玲¹

1 重庆大学光电技术及系统教育部重点实验室,重庆 400044
2 重庆大学光电工程学院智能感知技术中心(CIST),重庆 400044

Naked-eye Detection of Hepatitis B Surface Antigen Based on Catalase-Mediated Crosslinking Aggregation of Gold Nanoparticles

CHEN Rubing¹, HU Yongqin^1,2, CHEN Meizhu¹, AN Jia^1,2, LYU Ying¹, LIU Yufei^1,2, LI Dongling¹

1 Key Laboratory of Optoelectronic Technology & Systems, Ministry of Education, Chongqing University, Chongqing 400044, China
2 Center for Intelligent Sensing Technology (CIST), College of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China

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摘要开发高效、高灵敏度又适用于基层的简便、稳定、成本低的病毒标志物检测方法对病毒的早期诊断具有重要意义。本工作通过制备同时具有催化和特异性识别能力的双功能聚苯乙烯纳米球催化H₂O₂分解,剩余的H₂O₂通过交联剂诱导金纳米颗粒聚集,使溶液颜色从红色变为蓝色,实现对乙肝表面抗原的高效、高灵敏、高特异性可视化检测。肉眼检测限为0.5 ng/mL,借助酶标仪后的检测限为0.01 ng/mL。此外,HBsAg在血清中的回收率在88.17%~102.50%,相对标准偏差在2.01%~4.52%,表明所提出的免疫测定法适用于血清中的检测,在实际临床中具有广阔的应用前景。

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	陈如冰
	胡永琴
	陈美珠
	安佳
	吕颖
	刘玉菲
	李东玲

关键词: 可视化检测乙肝表面抗原催化金纳米颗粒聚集

Abstract: Developing efficient, highly sensitive, simple, stable, and low-cost virus markers detection productsfor local clinics is of great emphasis in the early diagnosis and treatment of the virus. In this paper, the bifunctional polystyrene nanospheres with both catalytic and specific recognition capabilities are prepared to catalyze the decomposition of H₂O₂, and the remaining H₂O₂ will induce the aggregation of gold nanoparticles through the crosslinking agents, which causes the color of the solution to change from red to blue, so as to achieve the high-efficiency, high-sensitivity and high-specificity naked-eye detection of hepatitis B surface antigen. The detection limit of the naked-eye was recorded as 0.5 ng/mL and the detection limit assisted with microplate reader is 0.01 ng/mL. In addition, the recovery rate of HBsAg in serum is between 88.17%—102.50%, and the relative standard deviation is between 2.01%—4.52%, indicating that the proposed immunoassay is suitable for the detection in serum and has great potential in actual clinical practice.

Key words: naked-eye detection hepatitis B surface antigen catalysis gold nanoparticles aggregation

出版日期: 2022-03-10 发布日期: 2022-03-08

ZTFLH:

Q55

基金资助: 中央高校基本科研业务费专项资金(2019CDQYGD020;2019CDCGGD304);国家重点研发开发项目(2016YFE0125200);国家自然科学基金(61927818;62005030)

通讯作者: Yufei.Liu@cqu.edu.cn; lidongling@cqu.edu.cn

作者简介: 陈如冰,于2018年9月至2021年6月在重庆大学攻读仪器科学与技术专业学术硕士学位,主要从事生物传感器的研究。
刘玉菲,重庆大学光电工程学院教授,分别于2003年、2006年和2011年获得北京大学、中国科学院和赫瑞-瓦特大学(Heriot-Watt University)的物理学理学学士学位、微电子学和工程电子学硕士学位及电子工程博士学位,其研究重点为MEMS技术、先进传感技术及便携式诊断技术等。
李东玲,重庆大学光电工程学院高级工程师,主要从事新型微纳器件及特色加工工艺、智能感知技术的研究。

引用本文:

陈如冰, 胡永琴, 陈美珠, 安佳, 吕颖, 刘玉菲, 李东玲. 基于过氧化氢酶介导金纳米颗粒交联聚集的乙肝表面抗原可视化检测[J]. 材料导报, 2022, 36(5): 21020098-4.
CHEN Rubing, HU Yongqin, CHEN Meizhu, AN Jia, LYU Ying, LIU Yufei, LI Dongling. Naked-eye Detection of Hepatitis B Surface Antigen Based on Catalase-Mediated Crosslinking Aggregation of Gold Nanoparticles. Materials Reports, 2022, 36(5): 21020098-4.

链接本文:

http://www.mater-rep.com/CN/10.11896/cldb.21020098 或 http://www.mater-rep.com/CN/Y2022/V36/I5/21020098

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