Preparation and Properties of Conductive Hydrogel Scaffolds Based on Chitosan/Xanthan Gum Interpenetrating Networks
ZHOU Xin1,2, GUAN Shui1,2,3,*, SUN Changkai3,*
1 State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China 2 Dalian R & D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China 3 Research & Educational Center for the Control Engineering of Translational Precision Medicine (R-ECCE-TPM), School of Biomedical Engineering, Dalian University of Technology, Dalian 116024, Liaoning, China
Abstract: Conductive scaffolds are used in neural tissue engineering, which is beneficial to cell proliferation and growth, but the conductive polymers inside the scaffolds usually bring cytotoxicity and cell adhesion problems caused by hydrophobic groups. In this study, a novel PEDOT-HA/CS/XG interpenetrating network conductive hydrogel scaffold was successfully prepared by combining xanthan gum (XG) with chitosan (CS) and introducing hyaluronic acid-doped poly-3,4-ethylene dioxythiophene (PEDOT-HA) conductive nanoparticles. In the experiment, gluconolactone (GDL) was used to dissolve CS to realize the combination of CS and XG, and the gelation time was shortened by optimizing the content of GDL. The introduction of PEDOT-HA conductive nanoparticles gave the scaffold material conductivity, and the effect of PEDOT-HA content on the porosity, electrical conductivity and water absorption of the scaffold material was determined, besides the degradability, rheology, thermal stability and mechanical properties of the scaffold were investigated. The results showed that the introduction of PEDOT-HA improved the porosity, electrical conductivity, elasticity and mechanical strength of the hydrogel, and the water absorption rate of the PEDOT-HA/CS/XG conductive hydrogel scaffold was between 2 575%—3 949%, and it has suitable degradation rate and thermal stability. Cell adhesion rate detection and scanning electron microscope (SEM) observation results showed that the introduction of PEDOT-HA was beneficial to the adhesion and growth of PC12 cells and formed a staggered network structure. The results of cell viability detection and fluorescence staining showed that PC12 cells maintained a good proliferation activity on the conductive hydrogel scaffold. After 5 d of culture, the cell viability on the 10% PEDOT-HA/CS/XG scaffold could reach the highest level of 120.42% in the control group, which proves that the PEDOT-HA/CS/XG conductive hydrogel scaffold has good biocompatibility and demonstrates potential for application in neural tissue engineering.
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2023, Vol. 37 
