Collagen Coating Improves the Bioactivity of Apatite Substrate in Osteoblast Proliferation and Differentiation
MA Pengyi1,2, LI Chen1,2, Ouaskioud Oumaima1,2, REN Li1,2,*
1 Key Laboratory of Flexible Electronics of Zhejiang Province, Ningbo Institute of Northwestern Polytechnical University, Ningbo 315103, Zhejiang, China 2 Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China
Abstract: Bone matrix is mainly composed of type I collagen and apatite and undergoes renewal during remodeling. During this process, reversal cells recondition the resorbed surfaces left by osteoclasts and deposit a thin layer of proteins (mainly collagen) to form reversal lines, this is an obligatory step in the link between bone resorption and formation. The collagen layer is critical for osteoblast growth and the formation of new bone matrix. In order to verify the hypothesis, we modified a widely accepted simulated body fluid by increasing the relative proportion of Ca2+ and HPO42- to effectively generate a continuous bone-like apatite substrate. Then the substrates with or without collagen pre-coating were used for MC3T3-E1 osteoblast-like cells culturing and inducing them to differentiate. The cell attachment, proliferation, and the expression of two osteogenic markers were detected. The results indicated that osteoblasts on an apatite substrate showed retracted morphologies and delayed proliferation and differentiation, and tended to secrete more collagen before they were committed to differentiation. After collagen coating of apatite substrates, osteoblasts maintained spreading morphology and higher proliferation rates, and expressed high levels of alkaline phosphatase after differentiation induction. This most probably indicated that the collagen pre-coated on apatite substrates, as deposited on the resorbed surface by reversal cells during bone remodeling, is important for osteoblast attachment, proliferation, and differentiation.
1 Sroga G E, Karim L, Colón W, et al. Molecular & Cellular Proteomics, 2011, 10(9), M110. 006718. 2 Addison W N, Nelea V, Chicatun F, et al. Bone, 2015, 71, 244. 3 Everts V, Delaissé J M, Korper W, et al. Journal of Bone and Mineral Research, 2002, 17(1), 77. 4 Parfitt A M. Metabolic Bone Disease and Related Research, 1982, 4(1), 1. 5 Domon T, Suzuki R, Takata K, et al. Annals of Anatomy, 2001, 183(2), 103. 6 Perrier A, Dumas V, Linossier M T, et al. Bone, 2010, 47(1), 23. 7 Matsuo K, Irie N. Archives of Biochemistry Biophysics, 2008, 473(2), 201. 8 Kristensen H B, Andersen T L, Marcussen N, et al. American Journal of Pathology, 2014, 184(3), 778. 9 Abdelgawad M E, Søe K, Andersen T L, et al. Bone, 2014, 67, 181. 10 He Z, Zhai Q, Hu M, et al. Journal of Orthopaedic Translation, 2015, 3(1), 1. 11 Ducheyne P, Qiu Q. Biomaterials, 1999, 20(23-24), 2287. 12 Cholas R, Padmanabhan S K, Gervaso F, et al. Materials Science & Engineering C-Materials for Biological Applications, 2016, 63, 499. 13 Lin K, Xia L, Gan J, et al. ACS Applied Materials & Interfaces, 2013, 5(16), 8008. 14 Wei G, Ma P X. Biomaterials, 2004, 25(19), 4749. 15 Chou Y F, Huang W, Dunn J C, et al. Biomaterials, 2005, 26(3), 285. 16 Chaudhuri O, Gu L, Klumpers D, et al. Nature Materials, 2016, 15(3), 326. 17 Yang X B, Bhatnagar R S, Li S, et al. Tissue Engineering, 2004, 10(7-8), 1148. 18 Chen Y, Mak A F T, Wang M, et al. Surface & Coatings Technology, 2006, 201(3-4), 575. 19 Murshid S A, Kamioka H, Ishihara Y, et al. Journal of Bone and Mine-ral Metabolism, 2007, 25(3), 151. 20 Tsai S W, Cheng Y H, Chang Y, et al. Journal of the Taiwan Institute of Chemical Engineers, 2010, 41(3), 247. 21 Ren L, Liu W, Wang Y, et al. Analytical Chemistry, 2013, 85(1), 235. 22 Qu H, Wei M. Journal of Biomedecal Materials Research Part B-Applied Biomaterials, 2008, 87(1), 204. 23 Sato K, Kumagai Y, Tanaka J. Journal of Biomedical Materials Research, 2000, 50(1), 16. 24 Murphy W L, Mooney D J. Journal of the American Chemical Society, 2002, 124(9), 1910. 25 Yang C, Li Y, Nan K. Materials Research Bulletin, 2013, 48(3), 1128. 26 Ye Y J, Yin D C, Shang P. Applied Surface Science, 2010, 256(24), 7535. 27 Kim H M, Miyazaki T, Kokubo T, et al. Key Engineering Materials, 2001, 192-195, 47. 28 Lausch A J, Quan B D, Miklas J W, et al. Advanced Functional Materials, 2013, 23(39), 4906. 29 Charles L F, Kramer E R, Shaw M T, et al. Journal of the Mechanical Behavior of Biomedical Materials, 2013, 17, 269. 30 Bigi A, Boanini E, Panzavolta S, et al. Journal of Biomedical Materials Research, 2002, 59(4), 709. 31 Yang L, Li L, Tu Q, et al. Analytical Chemistry, 2010, 82(15), 6430. 32 Posner A S. Physiological Reviews, 1969, 49(4), 760. 33 Mahamid J, Aichmayer B, Shimoni E, et al. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(14), 6316. 34 Raafat A I, Saad Eldin A A, Salama A A, et al. Journal of Applied Polymer Science, 2013, 128(3), 1697. 35 Fu R, Liu Q, Song G, et al. Biomedical Materials, 2013, 8(5), 055005. 36 Folkman J, Moscona A. Nature, 1978, 273(5661), 345. 37 Ingber D E. Proceedings of the National Academy of Sciences of the United States of America, 1990, 87(9), 3579. 38 Yan C, Sun J, Ding J. Biomaterials, 2011, 32(16), 3931. 39 Dalby M J, Gadegaard N, Oreffo R O. Nature Materials, 2014, 13(6), 558. 40 Franceschi R T, Iyer B S. Journal of Bone and Mineral Research, 1992, 7(2), 235. 41 Connelly J T, Gautrot J E, Trappmann B, et al. Nature Cell Biology, 2010, 12(7), 711. 42 Singhvi R, Kumar A, Lopez G P, et al. Science, 1994, 264(5159), 696. 43 Han W, Zhao J, Tu M, et al. Journal of Applied Polymer Science, 2013, 128(3), 1332. 44 Qi X, Huang Y, Han D, et al. Biomedical Materials, 2016, 11(2), 025005. 45 Bellucci D, Sola A, Gentile P, et al. Journal of Biomedical Materials Research Part A, 2012, 100(12), 3259. 46 Liu Q, Cen L, Yin S, et al. Biomaterials, 2008, 29(36), 4792.
渝公网安备50019002502923号 版权所有：《材料导报》编辑部
2024, Vol. 38 
