金属有机骨架材料固定化酶的研究进展

材料导报

2021, Vol. 35

Issue (z2): 285-293

金属与金属基复合材料

金属有机骨架材料固定化酶的研究进展

徐冉^1,2, 李智慧^1,2, 吴一楠^1,2, 李风亭^1,2

1 同济大学环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海 200092
2 上海污染控制与生态安全研究院,上海 200092

Research Advances in Enzyme Immobilization on Metal-organic Frameworks

XU Ran^1,2, LI Zhihui^1,2, WU Yinan^1,2, LI Fengting^1,2

1 State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
2 Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China

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摘要金属有机骨架材料(MOFs)凭借其较高的比表面积和孔体积、可设计和调控的孔径及结构,以及化学和热稳定性等特点,克服了传统固定化酶载体的孔径尺寸不可控、制备成本高、酶浸出、产物稳定性差等不足,近年来成为一类新型酶固定化载体。首先,本文分类总结了MOFs固定化酶的合成策略,包括后合成包装和从头合成封装(仿生矿化、共沉淀和机械化学封装);然后进一步介绍了多级孔MOFs的孔道设计策略及其固定化酶体系。这种具备分级孔道结构的MOFs用于固定化酶既可以保证酶的较高负载率,又能提高酶催化底物的扩散速率;此外,本文还介绍了MOFs共固定化多酶体系及具有类酶特性的仿生MOFs固定化酶方面的研究。MOFs特有的孔道结构可以大大缩短酶与底物之间的扩散距离,同时充分利用了酶级联反应的中间产物,可以显著提高酶催化活性;文章最后总结了MOFs固定化酶复合材料在生物传感和污染物催化净化领域的主要应用,提出了MOFs固定化酶研究中的一些瓶颈问题,以期为该材料的进一步研究和未来产业化提供借鉴和参考。

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关键词: 金属有机骨架材料固定化酶多级孔级联反应生物传感污染物催化

Abstract: The traditional immobilization matrices for enzymes have limitations of uncontrollable pore size distribution, high cost, leaching of enzymes, and poor stability. Metal-organic frameworks (MOFs) are considered as a new class of solid supports for enzyme immobilization due to their intrinsic properties, such as high surface area and pore volume, tunable pore size and structure, and chemical and thermal stability. Firstly, this review summarizes the preparation strategies for enzyme immobilization in MOF materials by the following two major categories, postsynthetic packaging and de novo encapsulation (biomimetic mineralization, co-precipitation, and mechanochemical encapsulation). Secondly, it introduces the design strategies of hierarchically porous MOFs for enzyme immobilization, which enable the achievement of high enzyme loading and the improvement of substrate diffusion rate. Thirdly, it introduces some advances on multienzyme systems in MOFs and biomimetic MOFs with enzyme-like activity for enzyme immobilization. The special pore structure of MOFs can greatly shorten the diffusion distance between enzyme and substrate, make full use of the intermediate products of enzyme cascade reaction, and therefore could significantly improve the catalytic activity of enzyme. Finally, the main applications of MOF-enzyme composites in biosensing and catalytic removal of pollutants are summarized, and some bottleneck problems are put forward to provide reference for the further research and future industrialization of MOF-enzyme composites.

Key words: metal-organic frameworks (MOFs) enzyme immobilization hierarchically pore structure cascade reaction biosensing pollutant catalysis

发布日期: 2021-12-09

ZTFLH:

O641.4

基金资助: 国家自然科学基金(21777119)

通讯作者: 51n@tongji.edu.cn

作者简介: 徐冉,同济大学环境科学与工程学院副教授、硕士研究生导师。2004年毕业于新加坡国立大学化学与环境工程系,获博士学位。主要从事水处理技术和绿色环境功能材料研究。在Journal of Environmental Quality、Chemical Engineering Journal、ACS Applied Materials and Interfaces、Bioresource Technology、《同济大学学报》等期刊发表论文70余篇,主持参与多项国家及省部级科研项目,多次获得省部级科技奖项,授权专利13项。
吴一楠,同济大学环境科学与工程学院副教授、博士研究生导师。2012年毕业于同济大学环境科学与工程专业获博士学位。主要从事多层次孔结构材料的合成、金属有机骨架材料的绿色宏量制备及先进应用基础研究。在Angewandte Chemie-International Edition、Small、Chemical Communications、Journal of Hazardous Materials等国际期刊发表论文50余篇,主持多项国家及省部级科研项目,授权发明专利7项。

引用本文:

徐冉, 李智慧, 吴一楠, 李风亭. 金属有机骨架材料固定化酶的研究进展[J]. 材料导报, 2021, 35(z2): 285-293.
XU Ran, LI Zhihui, WU Yinan, LI Fengting. Research Advances in Enzyme Immobilization on Metal-organic Frameworks. Materials Reports, 2021, 35(z2): 285-293.

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http://www.mater-rep.com/CN/ 或 http://www.mater-rep.com/CN/Y2021/V35/Iz2/285

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