Preparation of Hierarchical Macroporous/Mesoporous Silica and Its Application in Lipase Immobilization
LI Xiaoying, BAI Wenjing, TAO Kai, LIANG Yunxiao
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211
Abstract: Millimeter-sized hierarchical macroporous/mesoporous silica was prepared by using an epoxy resin macroporous polymer with three-dimensional (3D) skeletal structure as template. Pore structures and surface properties of the as-prepared materials were characterized by SEM, MIP, FTIR and N2 adsorption-desorption. Candida rugosa lipase (CRL) was immobilized on the macroporous/mesoporous silica by adsorption method. The effects of the initial CRL concentration, pH and immobilizing time on lipase immobilization were investigated, and the properties of free and immobilized lipase were studied. The results demonstrated that the macroporous/mesoporous silica had 3D continuous pass-through macroporous structure; its pore wall was constructed by continuous silica nano-film and had abundant mesopores. The specific surface area and porosity are 75.1 m2/g and 92.3%, respectively. The activity of immobilized lipase reaches 4 825 U/g under conditions of initial CRL concentration 0.6 mg/mL, pH of 8.0 and immobilizing time of 10 h. Compared with free CRL, the pH, thermal and storage stabilities of immobilized lipase were improved significantly. The immobilized lipase retained 68% residual activity after 8 consecutive operations. Therefore, this hierarchical macroporous/mesoporous silica was a good potential candidate for enzyme immobilization.
李笑迎,白文静,陶 凯,梁云霄. 大孔/介孔多级孔SiO2的制备及其在固定化脂肪酶中的应用[J]. 《材料导报》期刊社, 2018, 32(10): 1695-1700.
LI Xiaoying, BAI Wenjing, TAO Kai, LIANG Yunxiao. Preparation of Hierarchical Macroporous/Mesoporous Silica and Its Application in Lipase Immobilization. Materials Reports, 2018, 32(10): 1695-1700.
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2018, Vol. 32 
