Abstract: Photopolymerized hydrogel materials play a critical role in tissue engineering. In this study, we synthesized a fast photopolymerizable hydrogel material, acrylated poly (ethylene glycol)-co-poly (xylitol sebacate) copolymer (PEXS-A). Further, we particularly explored the synthesis process in regard of poly(ethylene glycol) (PEG) dosage, acylating agent selection and dialysis duration. The characterization of cytocompatibility and biodegradation properties of the hydrogel was carried out as well. The poly (ethylene glycol) dosage was determined by comparing the water solubility of the prepolymer. The structure of the material was analyzed by nuclear magnetic resonance spectroscopy (1H-NMR) and the degree of acrylated material was calculated. After dialysis for 0 h, 24 h, 48 h, 72 h, the hydrogels were cultured together with the cells for the sake of detecting the cell survival rate, in which the CCK-8 cytotoxicity test kit was adopted. Specifically, the cells were embedded in hydrogel materials and cultured in cell culture medium for 48 h, then the survival condition of cells were observed by fluorescence staining with live/dead cell double staining kit. Moreover, the mass alteration of PEXS-A hydrogel during the biodegradation process in phosphate buffered saline (PBS) under 37 ℃ for 28 days was tested. According to the results, PEXS prepolymer with highest proportion of hydroxyl groups, soluble in water, could be obtained, when the mass fraction of PEG was 60%. The PEXS-A exhibited a 91.61% high acrylation degree, which was able to polymerize into hydrogel within 1 min under ultraviolet light. After a 48 h of water dialysis, PEXS-A prepolymer showed similar cytotoxicity to the control material PDLLA. There was exceptionally high survival rate of cells that embedded in PEXS-A hydrogel curing for 48 h. Besides, PEXS-A hydrogel possessed satisfactory biodegradability, and a 52.85%±0.64% degradation of mass could be observed after 28 days in vitro.
王异彩, 张甜, 李媛. 光聚合PEXS-A水凝胶材料的合成及包埋活细胞的性能[J]. 材料导报, 2020, 34(2): 2169-2173.
WANG Yicai, ZHANG Tian, LI Yuan. Synthesis of Photopolymerized PEXS-A Hydrogel and Its Performance of Embedding Living Cells. Materials Reports, 2020, 34(2): 2169-2173.
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2020, Vol. 34 
