In Vitro Cytotoxicity Evaluation of Cellulose Absorbable Hemostatic Materials
YU Yan1, MA Fengsen1, LU Jiajun2, CHEN Haibo2
1 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014; 2 Zhejiang Institute of Medical Device Testing, Hangzhou 310018
Abstract: In order to provide reference for clinical application of cellulose absorbable hemostatic materials, cytocompatibility of three absorbable hemostatic materials (Surgicel��, Yingjieer�� and Taikeling��) were evaluated by cytotoxicity testing in vitro. The effects of the three materials on the proliferation and the cytotoxicity of L929 cells were evaluated by MTT colorimetric method, cell morphology analysis, agar diffusion method and membrane diffusion method. The incomplete culture medium was added to every material at a ratio of sample/medium of 50.00 mg/mL. After 24 h, the extracts solution designated as 100% extracts was separated by centrifugation and decantation. The extracts was serially diluted to give 50.0%, 25.0%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, and 0.39% extracts. MTT colorimetric method combined with cell morphology analysis showed that each concentration of extract of Yingjieer�� had no significant cytotoxicity, while extracts of Surgicel�� and Taikeling�� had no significant cytotoxicity when their concentration was below 25.0%. Among the three materials, agar diffusion method and membrane diffusion method showed that Yingjieer�� had no significant cytotoxicity, but Surgicel�� and Taikeling�� had significant cytotoxicity. The cytocompatibilitys of the three different hemostatic materials were different, therefore it is important to select appropriate evaluation methods.
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2018, Vol. 32 
