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《材料导报》期刊社  2018, Vol. 32 Issue (1): 76-85    https://doi.org/10.11896/j.issn.1005-023X.2018.01.009
  物理   材料综述 |材料 |
生物材料细胞毒性评价研究进展
马凤森1(),喻炎1,章捷1,陈海波2
1 浙江工业大学药学院,生物制剂与材料实验室, 杭州 310014
2 浙江省医疗器械检验院, 杭州 310018
A State-of-the-art Review of Cytotoxicity Evaluation of Biomaterials
Fengsen MA1(),Yan YU1,Jie ZHANG1,Haibo CHEN2
1 Biologics and Biomaterials Laboratory, College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014
2 Zhejiang Institute of Medical Device Testing, Hangzhou 310018
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摘要 

随着生物材料在医药领域的应用越来越广泛,其临床前的生物安全性评价亦愈加重要。细胞毒性检测是一种体外试验方法,与体内试验方法相比具有简便、快速、重复性好、干扰少、可避免伦理问题等优点,已成为生物材料临床应用前的常规检测项目,在材料的生物相容性评价方面发挥着重要作用。随着现代细胞生物学与分子生物学技术的不断发展,细胞毒性评价试验中出现了一些国内外标准尚未收录的评价新指标与新方法。同时,材料不同的使用目的与使用部位以及新材料的不断涌现,决定了细胞毒性试验方法的多样性。就生物材料相关的细胞毒性试验方法、常用材料细胞毒性评价以及生物材料细胞毒性评价尚存在的问题作一综述,以为相关研究提供参考。

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马凤森
喻炎
章捷
陈海波
关键词:  生物材料  细胞毒性检测  评价指标  评价方法    
Abstract: 

Biomaterials are widely applied in medical field, and their biocompability evaluation before clinical application has become increasingly important. Cytotoxicity assay is an experimental method in vitro, which takes the advantages of simplicity, high speed, good repeatability and no ethical problems compared with the method in vivo. Accordingly, cytotoxicity assay has become a conventional examination for biomaterials before the clinical application, and played a significant role in biocompatibility evaluation. With the rapid development of modern cell biology and molecular biology technology, some new evaluation indicators and methods are urged to be included in the cytotoxicity evaluation standards at home and abroad. Simultaneously, the diversity of cytotoxicity assay methods highly depends on the development of new materials, the different purposes and application locations. This review summarized the methods of cytotoxicity evaluation, the cytotoxicity tests of the most commonly used materials and the problems in current cytotoxicity evaluation of biomaterials, which aims to pave the way for the researchers in relative fields.

Key words:  biomaterials    cytotoxicity assay    evaluation indicators    evaluation methods
               出版日期:  2018-01-10      发布日期:  2018-01-10
ZTFLH:  TB39  
  R318.08  
基金资助: 浙江省科技项目(2016F50078)
作者简介:  马凤森:男, 1962年生, 教授, 硕士研究生导师, 研究方向为生物材料与制剂研究评价 Tel:0571-88871500 E-mail:merrigen@126.com
引用本文:    
马凤森,喻炎,章捷,陈海波. 生物材料细胞毒性评价研究进展[J]. 《材料导报》期刊社, 2018, 32(1): 76-85.
Fengsen MA,Yan YU,Jie ZHANG,Haibo CHEN. A State-of-the-art Review of Cytotoxicity Evaluation of Biomaterials. Materials Reports, 2018, 32(1): 76-85.
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http://www.mater-rep.com/CN/10.11896/j.issn.1005-023X.2018.01.009  或          http://www.mater-rep.com/CN/Y2018/V32/I1/76
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