Zn2+交联海藻酸钠抑菌缓释微球制备及其抑菌效应

doi:10.11896/cldb.21020040

材料导报

2022, Vol. 36

Issue (7): 21020040-7 https://doi.org/10.11896/cldb.21020040

高分子与聚合物基复合材料

Zn²⁺交联海藻酸钠抑菌缓释微球制备及其抑菌效应

李玉妍¹, 杨忠鑫¹, 陈南春¹, 莫胜鹏², 王秀丽³, 解庆林²

1 桂林理工大学材料科学与工程学院,广西桂林 541004
2 桂林理工大学环境科学与工程学院,广西岩溶地区水污染控制与用水安全保障协同创新中心, 广西桂林 541006
3 桂林理工大学化学与生物工程学院,广西桂林 541006

Preparation and Antibacterial Effect of Zn²⁺ Crosslinked Alginate Slow-release Microspheres

LI Yuyan¹, YANG Zhongxin¹, CHEN Nanchun¹, MO Shengpeng², WANG Xiuli³, XIE Qinglin²

1 School of Materials Science and Engineering, Guilin University of Technology, Guilin 541004, Guangxi, China
2 School of Environmental Science and Engineering, Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541006, Guangxi, China
3 School of Chemical and Biological Engineering, Guilin University of Technology, Guilin 541006, Guangxi, China

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摘要为提高二甲酸钾(KDF)在胃肠道内的利用率,本研究基于单因素实验采用响应面法优化设计数据,利用Zn²⁺交联海藻酸钠(ALG)制备出KDF缓释抑菌微球,并对微球的化学结构、微观形貌及热性能进行系统表征,以及测定微球的缓释抑菌性能。结果表明,微球的最佳配方为:ALG浓度1.5%(质量分数,下同),ZnSO₄浓度7.0%,表面活性剂0.8 mL。优化后的微球在肠道内的释放作用时间可延长至6 h,释放机制遵循一级动力学模型和Ritger-Peppas模型,显著减缓了KDF的释放速率;微球浓度为96 mg/mL时,其对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑菌率分别为88%、87%、67%,有效抑制了细菌的过度繁殖,是一种可在家畜饲养业广泛普及的新型抑菌剂。

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	李玉妍
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	解庆林

关键词: 二甲酸钾响应面法 Zn²⁺交联微球释放机制抑菌率

Abstract: To improve the utilization of potassium diformate (KDF) in gastrointestinal tract, this study, which was based on the single factor experiment, optimized the design data by response surface methodology. The KDF microspheres were prepared by Zn²⁺ cross-linked sodium alginate (ALG). The obtained microspheres were systematically characterized by chemical structure, morphology and thermal properties, and the sustained-release antibacterial properties of the microspheres were tested. The results showed that the optimal formulation of microspheres was the concentration of ALG was 1.5% (mass fraction, the same below), the concentration of ZnSO₄was 7.0%, and the surfactant was 0.8 mL. In vitro slow-release properties of microspheres showed that the release time was prolonged to 6 h in the intestinal tract, and the release mechanism followed the first-order kinetic model and Ritger-Peppas model, which significantly slowed down the release rate of KDF. In addition, when the concentration of microspheres was 96 mg/mL, the bacteriostatic rates against Escherichia coli, Staphylococcus aureus and Bacillus subtilis were 88%, 87% and 67%, respectively. It effectively inhibits the overreproduction of bacteria and it was a potential new antimicrobial agent which could be widely used in livestock feeding.

Key words: potassium diformate response surface methodology Zn²⁺cross-linked microspheres release mechanism bacteriostatic rate

发布日期: 2022-04-07

ZTFLH:

TB321

基金资助: 国家自然科学基金(51564008;51978189);广西环境污染控制理论与技术重点实验室研究项目(1801K002)

通讯作者: cnc@glut.edu.cn

作者简介: 李玉妍,2018年本科毕业于济宁学院材料化学专业,2021年硕士毕业于桂林理工大学材料科学与工程学院高分子化学与物理专业,主要从事沸石接枝二甲酸钾及二甲酸钾抑菌缓释微球的制备研究。
陈南春,桂林理工大学研究员,二级教授,教育部“长江学者奖励计划”特约通讯评审专家。主要从事矿物功能材料、环境功能材料和复合材料的教学与科研工作。2002年硕士毕业于桂林理工大学无机非金属材料专业。发表SCI/EI收录论文70余篇,获得国家授权发明专利14项。

引用本文:

李玉妍, 杨忠鑫, 陈南春, 莫胜鹏, 王秀丽, 解庆林. Zn²⁺交联海藻酸钠抑菌缓释微球制备及其抑菌效应[J]. 材料导报, 2022, 36(7): 21020040-7.
LI Yuyan, YANG Zhongxin, CHEN Nanchun, MO Shengpeng, WANG Xiuli, XIE Qinglin. Preparation and Antibacterial Effect of Zn²⁺ Crosslinked Alginate Slow-release Microspheres. Materials Reports, 2022, 36(7): 21020040-7.

链接本文:

http://www.mater-rep.com/CN/10.11896/cldb.21020040 或 http://www.mater-rep.com/CN/Y2022/V36/I7/21020040

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