基于丙烯酸-N-琥珀酰亚胺酯共聚物交联剂的壳聚糖水凝胶的生物相容性研究

doi:10.11896/cldb.22120119

材料导报

2024, Vol. 38

Issue (12): 22120119-10 https://doi.org/10.11896/cldb.22120119

高分子与聚合物基复合材料

基于丙烯酸-N-琥珀酰亚胺酯共聚物交联剂的壳聚糖水凝胶的生物相容性研究

杨水艳¹, 盛扬¹, 孙一新¹, 蔡仁钦^2,*, Mark Bradley³, 张嵘^1,*

1 常州大学材料科学与工程学院,江苏常州 213164
2 大博医疗科技有限公司基础研究院,福建厦门 361026
3 英国爱丁堡大学化学学院,英国爱丁堡 EH93JJ

Biocompatibility of Chitosan Hydrogels Based on Acrylic-N-Succinimidyl Ester Copolymer Crosslinker

YANG Shuiyan¹, SHENG Yang¹, SUN Yixin¹, CAI Renqin^2,*, Mark Bradley³, ZHANG Rong^1,*

1 School of Materials Science and Engineering, Changzhou University, Changzhou 213164, Jiangsu, China
2 Basic Research Institute, Double Medical Technology Inc., Xiamen 361026, Fujian, China
3 School of Chemistry, University of Edinburgh, Scotland EH93JJ, Edinburgh, UK

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摘要壳聚糖作为一种天然的氨基多糖,拥有良好的生物相容性和资源可再生性,但是较差的力学性能限制了其更广泛的应用。本研究以丙烯酸、N-乙烯基吡咯烷酮和丙烯酸-N-琥珀酰亚胺酯为单体聚合得到三元共聚物大分子交联剂,然后将其与壳聚糖溶液混合后制备壳聚糖水凝胶。采用红外、热重、SEM等对合成的聚合物及壳聚糖凝胶的组成、形貌和热稳定性进行了表征。结果表明,丙烯酸-N-琥珀酰亚胺酯共聚物的数均分子量为111 900,具有一定的水溶性,与壳聚糖形成的水凝胶有良好的力学性能、溶胀性能和水蒸气渗透速率。在PBS(pH=7.4)中交联的凝胶膜与酸性条件下制备的凝胶膜相比,热分解温度提高,水接触角变大,力学性能得到较大提升。细胞毒性实验表明壳聚糖凝胶有良好的生物相容性,人脂肪干细胞在壳聚糖凝胶膜上会形成细胞簇并分泌外泌体。将壳聚糖凝胶膜覆盖在脂肪干细胞上,细胞能正常生长并分泌外泌体。因此,壳聚糖凝胶具有负载外泌体后应用于皮肤敷料的潜力。

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关键词: 壳聚糖丙烯酸-N-琥珀酰亚胺酯水凝胶外泌体

Abstract: As a natural amino polysaccharide, chitosan has good biocompatibility and renewable resources, but its application is limited due to poor mechanical properties. In this study, acrylic acid, N-vinylpyrrolidone and acrylic acid N-succinimide ester were used as monomers to obtain terpolymer macromolecular crosslinker, which was mixed with chitosan solution to prepare chitosan hydrogel. The composition, morphology and thermal stability of the synthesized polymer and chitosan gel were characterized by infrared, thermogravimetric analysis and SEM. The results showed that the average molecular weight of acrylate-N-succinimide ester copolymer was 111 900, which was water soluble. The chitosan hydrogel with the copolymer crosslinker showed good mechanical properties, swelling properties and water vapor permeation rate. Compared with the gel membrane prepared under acidic conditions, the thermal decomposition temperature of the crosslinked gel membrane in PBS (pH=7.4) increased, the contact angle of water became larger, and the mechanical properties were greatly improved. Cytotoxicity experiments had shown that chitosan gels have good biocompatibility. Human adipose-derived stem cells formed cell clusters on the gels and secreted exosomes on the chitosan gel membrane. By covering the chitosan gel membrane over the stem cells, the cells grew normally and secreted exosomes. Chitosan gels therefore have the potential to be applied to skin dressings after loading exosomes.

Key words: chitosan acrylic acid-N-succinimide ester hydrogel exosome

出版日期: 2024-06-25 发布日期: 2024-07-17

ZTFLH:

TQ460.4

基金资助: 江苏省六大人才高峰创新团队(SWYY-CXTD-001);常州市科技局国际合作项目(CZ20190019)

通讯作者: *蔡仁钦,大博医疗科技股份有限公司中级工程师。2011年硕士毕业于四川大学。主要从事高分子材料相关医疗器械研发。cairenqin@double-medical.com
张嵘,常州大学教授,江苏省特聘教授。2003年博士毕业于英国谢菲尔德大学。主要从事生物医用聚合物的研发,包括可生物降解聚合物水凝胶、抗/灭菌聚合物材料以及可降解纳米凝胶药物载体等。发表研究论文70余篇。rzhang@cczu.edu.cn

作者简介: 杨水艳,常州大学硕士研究生。2020年6月在常州大学获得材料化学学士学位。目前在张嵘教授的指导下进行研究,主要致力于基于丙烯酸酯活性酯组织粘合剂的制备及基于壳聚糖凝胶结合外泌体敷料的研究。

引用本文:

杨水艳, 盛扬, 孙一新, 蔡仁钦, Mark Bradley, 张嵘. 基于丙烯酸-N-琥珀酰亚胺酯共聚物交联剂的壳聚糖水凝胶的生物相容性研究[J]. 材料导报, 2024, 38(12): 22120119-10.
YANG Shuiyan, SHENG Yang, SUN Yixin, CAI Renqin, Mark Bradley, ZHANG Rong. Biocompatibility of Chitosan Hydrogels Based on Acrylic-N-Succinimidyl Ester Copolymer Crosslinker. Materials Reports, 2024, 38(12): 22120119-10.

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http://www.mater-rep.com/CN/10.11896/cldb.22120119 或 http://www.mater-rep.com/CN/Y2024/V38/I12/22120119

1 Jiang Q, Zhou W, Wang J, et al. International Journal of Biological Macromolecules, 2016, 91, 85.
2 Lima T D P D L, Passos M F. Journal of Biomaterials Science:Polymer Edition, 2021, 32(14), 11.
3 Liang Y, He J, Guo B. ACS Nano, 2021, 15(8), 12687.
4 Wang L R, Zhou M Y, Xu T L, et al. Chemical Engineering Journal, 2022, 433(1), 134625.
5 Zhang X. Construction of polysaccharide based hydrogel dressings based on skin wound microenvironment for injury repair. Ph. D. Thesis, Beijing University of Chemical Technology, China, 2022(in Chinese).
张想. 基于皮肤创面微环境构建多糖基水凝胶敷料及其在损伤修复中的研究. 博士学位论文, 北京化工大学, 2022.
6 Gao Y P. Study on preparation and performance of GelMA-BSP hydrogel for promoting wound healing. Master's Thesis, Southwest Minzu University, China, 2021(in Chinese).
高元平. 促进创面愈合水凝胶GelMA-BSP的制备及性能研究. 硕士学位论文, 西南民族大学, 2021.
7 Kim E, Kim M H, Song J H, et al. International Journal of Biological Macromolecules, 2020, 154, 989.
8 Fei L, Zhao J, Deng C Y. Polymer Science Series A, 2020, 62(5), 494.
9 Ladeira N M B, Donnici C L, de Mesquita J P, et al. Journal of Polymer Research, 2021, 28(9), 335.
10 Yang J, Shen M, Luo Y, et al. Carbohydrate Polymers, 2021, 257, 117608.
11 Shi W, Hass B, Kuss M A, et al. Carbohydrate Polymers, 2020, 233(C), 115803.
12 Bao Y, He J, Song K, et al. Polymers, 2022, 14(4), 769.
13 Koev T T, Muñoz-García J C, Iuga D, et al. Carbohydrate Polymers, 2020, 249, 116834.
14 Michelini L, Probo L, Farè S, et al. Materials Letters, 2020, 272, 1873.
15 Peers S, Montembault A, Ladavière C. Journal of Controlled Release, 2020, 326, 150.
16 Liu Y, Sun Q, Yang X, et al. ACS Applied Materials & Interfaces, 2018, 10, 19730.
17 Solé I, Vílchez S, Montanyà N, et al. Journal of Industrial Textiles, 2019, 50, 526.
18 Hamedi H, Moradi S, Hudson S M, et al. Carbohydrate Polymers, 2018, 199, 445.
19 Mao H, Zhao S, He Y, et al. Carbohydrate Polymers, 2022, 285, 119254.
20 Ackah S, Xue S, Osei R, et al. Frontiers in Microbiology, 2022, 13, 828914.
21 Andrade D O J, Pérez-Álvarez L, Sáez-Martínez V, et al. International Journal of Biological Macromolecules, 2022, 203, 679.
22 Ara C, Jabeen S, Afshan G, et al. International Journal of Biological Macromolecules, 2022, 202, 177.
23 Liu Q, Zhang X, Zhang J. Frontiers in Oncology, 2022, 12, 898605.
24 Kurian T K, Banik S, Gopal D, et al. Molecular Biotechnology, 2021, 63(4), 1.
25 Sancho-Albero M, Medel-Martínez A, Martín-Duque P. RSC Advances, 2020, 10(40), 23975.
26 Shi H, Wang M, Sun Y, et al. Frontiers in Cell and Developmental Biology, 2021, 9, 736022.
27 Zhao D, Yu Z, Li Y, et al. Journal of Molecular Histology, 2020, 51(3), 251.
28 Azaryan E, Karbasi S, Zarban A, et al. Wound Repair and Regeneration, 2022, 30(5), 585.
29 Tao S W. Exosome from adipose-derived mesenchymal stem cells/chitosan hydrogel complex materials for enhancement of skin wound repair. Master's Thesis, Wuhan University of Science and Technology, China, 2022(in Chinese).
陶苏皖. 脂肪间充质干细胞外泌体/壳聚糖水凝胶复合敷料促进皮肤伤口修复. 硕士学位论文, 武汉科技大学, 2022.
30 Chen Y Q. The effect of injectable hydrogel loaded exosome on the osteogenic ability of adipose-derived stem cells. Master's Thesis, China Medical University, China, 2019(in Chinese).
陈义庆. 可注射水凝胶负载外泌体促进脂肪干细胞成骨能力的研究. 硕士学位论文, 中国医科大学, 2019.
31 Liu F, Zhang Y, Wang Y L, et al. Chinese Journal of Tissue Engineering Research, 2021, 25(16), 2479 (in Chinese).
刘冯, 张瑜, 王燕丽, 等. 中国组织工程研究, 2021, 25(16), 2479.
32 Xu N, Wang L, Guan J, et al. International Journal of Biological Macromolecules, 2018, 117, 102.
33 Nooshabadi V T, Khanmohamadi M, Valipour E, et al. Journal of Biomedical Materials Research Part A, 2020, 108(11), 2138.
34 Di Q, Andi Z, Na W, et al. Applied Materials Today, 2021, 108(11), 2138.
35 An Y, Lin S, Tan X, et al. Cell Proliferation, 2021, 54(3), e12993.
36 Cao G, Chen B, Zhang X, et al. Clinical, Cosmetic and Investigational Dermatology, 2020, 13, 957.
37 Liu F, An Y, Sun Y, et al. Stem Cell Reviews and Reports, 2022, 18, 2059.
38 Dehghan-Baniani D, Chen Y, Wang D, et al. Colloids and Surfaces B:Biointerfaces, 2020, 192, 111059.
39 Lopes M, Restni R, Carvalho M P, et al. Reactive & Functional Polymers, 2021, 161, 104846.
40 Luo Y, Cui L, Zou L, et al. Carbohydrate Polymers, 2022, 294, 119774.
41 Hou B N, Shen H L, Li J, et al. Materials Engineering, 2020, 48(4), 73(in Chinese).
候冰娜, 沈惠玲, 李进, 等. 材料工程, 2020, 48(4), 73.
42 Fernandes Q M, Melo K R T, Sabry D A, et al. Marine Drugs, 2015, 13(1), 141.
43 Dey S C, Al-Amin M, Rashid T U, et al. International Journal of Latest Research in Engineering and Technology, 2016, 2(2), 52.
44 Maria B, Luiza-Madalina G, Simona M, et al. Reactive & Functional Polymers, 2021, 170, 105094.
45 Yao H Y, Lin H R, Sue G P, et al. Polymers and Polymer Composites, 2019, 27(3), 155.
46 Zhang D, Zhou W, Wei B, et al. Carbohydrate Polymers, 2015, 125, 189.
47 Catanzano O, Gomez D'ayala G, D'agostino A, et al. Carbohydrate Polymers, 2021, 264, 117987.
48 Long C, Wang J, Gan W, et al. Frontiers in Surgery, 2022, 9, 1030288.
49 Ma J, Zhang Z F, Wang Y M, et al. Experimental Dermatology, 2022, 31(3), 362.

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