氧化石墨烯与氮掺杂氧化石墨烯量子点负载去氧地胆草内酯抑制肿瘤细胞的研究

doi:10.11896/cldb.22030289

材料导报

2023, Vol. 37

Issue (14): 22030289-7 https://doi.org/10.11896/cldb.22030289

无机非金属及其复合材料

氧化石墨烯与氮掺杂氧化石墨烯量子点负载去氧地胆草内酯抑制肿瘤细胞的研究

韩欣彤¹, 曹阳^1,2, 文峰¹, 高助威³, 李成欣³, 于晓龙^1,*

1 海南大学材料科学与工程学院,海口 570228
2 琼台师范学院海南省儿童认知与行为发展重点实验室(筹),海口 571127
3 海南大学化学工程与技术学院,海口 570228

Study on the Inhibition of Tumor Cells by Deoxyelephantopin Loaded on Graphene Oxide Versus Nitrogen-doped Graphene Oxide Quantum Dots

HAN Xintong¹, CAO Yang^1,2, WEN Feng¹, GAO Zhuwei³, LI Chengxin³, YU Xiaolong^1,*

1 School of Materials Science and Engineering, Hainan University, Haikou 570228, China
2 Key Laboratory of Child Cognition & Behavior Development of Hainan Province, Qiongtai Normal University, Haikou 571127, China
3 School of Chemical Engineering and Technology, Hainan University, Haikou 570228, China

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摘要以膀胱肿瘤细胞UMUC-3为模型,研究了氧化石墨烯(GO)和氮掺杂氧化石墨烯量子点(NGQD)负载抗癌药物去氧地胆草内酯(DEO)体外抑制肿瘤细胞的效果。分别用Hummers法制备GO,球磨法制备NGQD,并通过超声负载的方法将DEO与GO、NGQD进行接枝。采用傅里叶变换红外光谱仪分析DEO与GO、NGQD的接枝机理,通过扫描电子显微镜分析其微观结构和元素分布。使用MTT比色法测试肿瘤细胞在GO-DEO、NGQD-DEO作用下的增殖情况,使用免疫荧光技术进一步分析NGQD-DEO抑制率更高的原因。结果表明,GO与NGQD均可通过共价键接枝的方式负载DEO,同时对比得到,GO-DEO、NGQD-DEO负载组均可通过破坏细胞骨架的方式提高对肿瘤细胞的抑制率,其中NGQD负载15 μmol/L DEO且时间为48 h和72 h时对肿瘤细胞骨架的破坏效果最佳。

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关键词: 药物载体去氧地胆草内酯膀胱癌细胞细胞增殖细胞骨架

Abstract: Using bladder tumor cell UMUC-3 as a model, the anti-tumor activity of deoxyelephantopin (DEO) loaded on graphene oxide (GO) and nitrogen-doped graphene oxide quantum dots (NGQD) in vitro was studied. GO was prepared by Hummers method, NGQD was prepared by ball milling method, and DEO was connected onto GO and NGQD by ultrasonic loading. The grafting mechanisms of GO-DEO and NGQD-DEO were analyzed by FTIR. The microstructure and element distribution of GO-DEO and NGQD-DEO were analyzed by scanning electron microscope. MTT colorimetry was used to test the cell proliferation induced by GO-DEO and NGQD-DEO, and immunofluorescence was used to further analyze the reasons for NGQD-DEO inhibition. The results show that both GO and NGQD can load DEO by covalent bond grafting. In comparison, GO-DEO and NGQD-DEO groups can enhance the inhibition of tumor cells by destroying the cytoskeleton, with NGQD loading 15 μmol/L DEO for 48 h and 72 h were the best time to destroy the cytoskeleton.

Key words: drug carrier deoxyelephantopin bladder cancer cell cell proliferation cytoskeleton

出版日期: 2023-07-25 发布日期: 2023-07-24

ZTFLH:

R318.08

基金资助: 海南省省级平台项目海南省生物医学材料研发创新服务平台(筹)资助项目(RZ2000007005)

通讯作者: *于晓龙,海南大学材料科学与工程学院副教授、硕士研究生导师。2008 年毕业于中山大学理工学院凝聚态物理专业,获得理学博士学位,2012 年清华大学材料系博士后出站。目前主要从事生物医用材料方面的研究,先后主持或参与国家科技计划973和863项目、国家科技支撑计划课题项目、省国际合作专项、省自然科学基金等课题五项,已发表高水平学术论文30余篇。获得2011年世界生物医用材料大会(希腊)“杰出青年科学家奖”、生物医用材料国际会议(海南)“优秀青年科学家奖”。yuxiaolong@hainanu.edu.cn

作者简介: 韩欣彤,2015年6月于海南大学获得工学学士学位,现为海南大学材料科学与工程学院硕士研究生,在于晓龙副教授的指导下进行研究。目前主要研究领域为生物材料。

引用本文:

韩欣彤, 曹阳, 文峰, 高助威, 李成欣, 于晓龙. 氧化石墨烯与氮掺杂氧化石墨烯量子点负载去氧地胆草内酯抑制肿瘤细胞的研究[J]. 材料导报, 2023, 37(14): 22030289-7.
HAN Xintong, CAO Yang, WEN Feng, GAO Zhuwei, LI Chengxin, YU Xiaolong. Study on the Inhibition of Tumor Cells by Deoxyelephantopin Loaded on Graphene Oxide Versus Nitrogen-doped Graphene Oxide Quantum Dots. Materials Reports, 2023, 37(14): 22030289-7.

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http://www.mater-rep.com/CN/10.11896/cldb.22030289 或 http://www.mater-rep.com/CN/Y2023/V37/I14/22030289

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