磁控溅射纳米银含量对钛种植体抗菌性的影响

doi:10.11896/cldb.22030032

材料导报

2023, Vol. 37

Issue (16): 22030032-6 https://doi.org/10.11896/cldb.22030032

金属与金属基复合材料

磁控溅射纳米银含量对钛种植体抗菌性的影响

程培雪¹, 马迅¹, 刘平¹, 王静静¹, 马凤仓¹, 张柯¹, 陈小红¹, 刘剑楠^2,*, 李伟^1,*

1 上海理工大学材料与化学学院,上海 200093
2 上海交通大学医学院附属上海第九人民医院,上海 200011

Effect of Magnetron Sputtering Nano-Ag Content on Antibacterial Properties of Titanium Implants

CHENG Peixue¹, MA Xun¹, LIU Ping¹, WANG Jingjing¹, MA Fengcang¹, ZHANG Ke¹, CHEN Xiaohong¹, LIU Jiannan^2,*, LI Wei^1,*

1 School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, China
2 Shanghai Ninth People's Hospital Affiliated to School of Medicine of Shanghai Jiao Tong University, Shanghai 200011, China

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摘要纯钛因具有优良的生物相容性,被广泛应用于口腔种植相关领域,但其本身并不具备抗菌性能,为改善钛种植体表面的抗菌性,采用磁控溅射法在钛种植体表面制备Ti-Ag纳米复合涂层,研究了涂层中纳米Ag含量对具核梭杆菌(Fusobacterium nucleatum, Fn)抗菌性能的影响。分析了样品的表面形貌、粗糙度和水接触角;对Ag⁺释放进行了检测;采用CCK-8法检测材料的细胞毒性;将各组样品与具核梭杆菌共培养,检测材料的抗菌性能。结果表明,载Ag复合涂层成功沉积在Ti片表面,并且随着纳米Ag含量的增加,样品的表面粗糙度和水接触角均增大。该Ti-Ag纳米复合涂层对小鼠L929细胞未表现出细胞毒性,符合生物安全标准。抗菌实验结果表明,随着纳米Ag含量的增加,Ag⁺释放量增加,涂层的抗菌效果也增强。可见,Ti-Ag纳米复合涂层可有效抑制Fn的生长,有望提高钛种植体的抗菌性能,为其临床运用奠定了实验基础。

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	程培雪
	马迅
	刘平
	王静静
	马凤仓
	张柯
	陈小红
	刘剑楠
	李伟

关键词: 磁控溅射钛种植体纳米银细胞毒性抗菌性

Abstract: Pure titanium is widely used in oral implant-related fields because of its excellent biocompatibility, but it does not have antibacterial properties by itself. In order to improve the antibacterial properties of titanium implants surfaces, this paper used magnetron sputtering to prepare Ti-Ag nanocomposite coating on the surface of titanium implants and investigated the effect of nano-Ag content in the coating on the antibacterial properties of Fusobacterium nucleatum (Fn). The surface morphology, roughness and water contact angle of the samples were analyzed; the Ag⁺ release was examined; the cytotoxicity of the materials was examined by the CCK-8 method; and each group of samples were co-cultured with Fusobacterium nucleatum to examine the antibacterial properties of the materials. The results showed that the Ag-loaded composite coating was successfully deposited on the Ti sheet surface, and the surface roughness and water contact angle of the samples increased with the increase of nano-Ag content. The Ti-Ag nanocomposite coating did not exhibit cytotoxicity to mouse L929 cells and met biosafety standards. The results of antibacterial experiments showed that with the increase of nano-Ag content, the Ag⁺ release increased and the antibacterial effect of the coating was enhanced. It is concluded that the Ti-Ag nanocomposite coating can effectively inhibit the growth of Fn, which is expected to improve the antibacterial performance of titanium implants and lay an experimental foundation for its clinical application.

Key words: magnetron sputtering titanium implant nano-Ag cytotoxicity antibacterial property

出版日期: 2023-08-25 发布日期: 2023-08-14

ZTFLH:

TB34

基金资助: 国家自然科学基金(51971148)

通讯作者: ^*刘剑楠,上海交通大学医学院附属第九人民医院口腔颌面头颈肿瘤科副主任医师,联合培养导师。2012年吉林大学口腔医学(本硕连读)专业毕业,2015年上海交通大学医学院口腔临床医学博士毕业后到上海交通大学医学院附属第九人民医院口腔颌面头颈肿瘤科工作至今。目前主要专注于头颈肿瘤发生、发展的研究及下颌骨重建关键技术的“产医研”转化。以第一和通讯作者身份发表SCI论文13篇、中文核心论文5篇,授权1项国际专利、6项发明专利、7项实用新型专利。荣获2019年国家科技进步二等奖。laurence_ljn@163.com
李伟,上海理工大学材料与化学学院教授,博士研究生导师,上海市高校“东方学者”特聘教授,上海市“曙光学者”。2008年上海交通大学材料物理与化学专业博士毕业后到上海理工大学工作至今,目前主要从事功能薄膜与涂层、生物医用材料、高熵合金材料、物理气相沉积(PVD)技术及其应用相关的研究。在Scripta Materialia、Scientific Reports、Materials Research Letters等国内外杂志发表学术论文80余篇,其中被SCI收录论文近60篇,申请国家发明专利38项,已授权26项。荣获2020年上海市科技进步奖二等奖(排名第一)、2019年上海市科技进步奖一等奖、2017年中国机械工业科学技术奖一等奖。liwei176@usst.edu.cn

作者简介: 程培雪,2018年7月于上海电机学院获得工学学士学位。现为上海理工大学材料与化学学院硕士研究生,在李伟教授的指导下进行研究。目前主要研究领域为钛种植体表面抗菌涂层。

引用本文:

程培雪, 马迅, 刘平, 王静静, 马凤仓, 张柯, 陈小红, 刘剑楠, 李伟. 磁控溅射纳米银含量对钛种植体抗菌性的影响[J]. 材料导报, 2023, 37(16): 22030032-6.
CHENG Peixue, MA Xun, LIU Ping, WANG Jingjing, MA Fengcang, ZHANG Ke, CHEN Xiaohong, LIU Jiannan, LI Wei. Effect of Magnetron Sputtering Nano-Ag Content on Antibacterial Properties of Titanium Implants. Materials Reports, 2023, 37(16): 22030032-6.

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http://www.mater-rep.com/CN/10.11896/cldb.22030032 或 http://www.mater-rep.com/CN/Y2023/V37/I16/22030032

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