Investigation of the Bioactive Glass on Wound Repair After Mineralization in Vitro for Different Time
HU Fang1, WANG Jian1, QIU Yuexiu1,2, GUO Chuangzhou1, LIU Mingdong1
1 Beijing Best Life Regenerative Medicine Technology Co., Ltd., Beijing 101200, China 2 Central Iron and Steel Research Institute, Beijing 100081, China
Abstract: This work takes a new type of biomedical material—bioactive glass as the main research object. The bioactive glass was mineralized in vitro for different time. Nitrogen adsorption, SEM and X-ray diffraction were employed to characterize the specific surface area, microstructure and phase before and after mineralization of the bioactive glass. Through the in vitro mineralization test, and the establishment of Guizhou pig skin wound surface model, the wound healing time and the healing rate were counted, as well as the HE staining analysis was performed to explore the difference in the bioactive glass on wound repair after in vitro mineralization for different time. Studies showed that the original specific surface area of bioactive glass was 186.8 m2/g, and regular three-dimensional network structure of carbonated hydroxyapatite was formed after 24 hours of immersion in simulated body fluids, and its specific surface area was up to 250.45 m2/g; the specific surface area of three-dimensional network structure treated for 48 hours was 472.30 m2/g, 2.5 times that of untreated materials. The original bioactive glass caused the wound surface of 5 cm×5 cm×4 cm to fill the whole wound surface with new tissue on (19.33±0.37) days, the repair of the wound surface required (22.16±0.65)days and (25.5±0.25)days with 24 hours and 48 hours of mineralization,. The bioactive glass group without mineralization could significantly promote wound healing, and the difference was statistically significant (P<0.05). Histological analysis showed that bioactive glass without mineralization could reduce inflammatory response, promote rapid and orderly growth of fibroblasts and capillaries, and complete new tissue structure.
胡方, 王健, 仇越秀, 郭创洲, 刘明东. 生物活性玻璃不同时间体外矿化对创伤修复的影响[J]. 材料导报, 2020, 34(Z2): 142-146.
HU Fang, WANG Jian, QIU Yuexiu, GUO Chuangzhou, LIU Mingdong. Investigation of the Bioactive Glass on Wound Repair After Mineralization in Vitro for Different Time. Materials Reports, 2020, 34(Z2): 142-146.