Please wait a minute...
材料导报  2020, Vol. 34 Issue (Z2): 142-146    
  无机非金属及其复合材料 |
生物活性玻璃不同时间体外矿化对创伤修复的影响
胡方1, 王健1, 仇越秀1,2, 郭创洲1, 刘明东1
1 北京幸福益生再生医学科技有限公司,北京 101200
2 钢铁研究总院, 北京 100081
Investigation of the Bioactive Glass on Wound Repair After Mineralization in Vitro for Different Time
HU Fang1, WANG Jian1, QIU Yuexiu1,2, GUO Chuangzhou1, LIU Mingdong1
1 Beijing Best Life Regenerative Medicine Technology Co., Ltd., Beijing 101200, China
2 Central Iron and Steel Research Institute, Beijing 100081, China
下载:  全 文 ( PDF ) ( 10307KB ) 
输出:  BibTeX | EndNote (RIS)      
摘要 本工作以一种新型的生物医用材料——生物活性玻璃为主要研究对象,对生物活性玻璃进行了不同时间的体外矿化,采用氮气吸附法、扫描电镜和X射线衍射分析技术对生物活性玻璃的比表面积、微观形貌和矿化前后物相进行了分析;通过体外矿化试验和建立贵州猪皮肤创面模型对创面愈合时间和愈合率进行了统计以及HE染色分析, 探讨了生物活性玻璃在不同时间体外矿化后对创伤修复的促进作用。研究表明:生物活性玻璃的原始比表面积为186.8 m2/g,在模拟体液中浸泡24 h即可形成规则的碳酸羟基磷灰石三维网状结构,其比表面积可达250.45 m2/g,经48 h处理的三维网状结构比表面积为472.30 m2/g,是未处理材料的2.5倍。原始生物活性玻璃促使5 cm×5 cm×4 cm创面在(19.33±0.37) d新生组织填充整个创面,经过24 h和48 h矿化后修复创面分别需要(22.16±0.65)d和(25.5±0.25)d,未经矿化的生物活性玻璃组能显著促进创面愈合,差异具有统计学意义(P<0.05)。组织学分析表明:未经过矿化处理的生物活性玻璃能减轻炎症反应,促进成纤维细胞和毛细血管快速有序生长,新生组织结构完整。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
胡方
王健
仇越秀
郭创洲
刘明东
关键词:  生物活性玻璃  三维网状结构  比表面积  体外矿化  创伤修复    
Abstract: This work takes a new type of biomedical material—bioactive glass as the main research object. The bioactive glass was mineralized in vitro for different time. Nitrogen adsorption, SEM and X-ray diffraction were employed to characterize the specific surface area, microstructure and phase before and after mineralization of the bioactive glass. Through the in vitro mineralization test, and the establishment of Guizhou pig skin wound surface model, the wound healing time and the healing rate were counted, as well as the HE staining analysis was performed to explore the difference in the bioactive glass on wound repair after in vitro mineralization for different time. Studies showed that the original specific surface area of bioactive glass was 186.8 m2/g, and regular three-dimensional network structure of carbonated hydroxyapatite was formed after 24 hours of immersion in simulated body fluids, and its specific surface area was up to 250.45 m2/g; the specific surface area of three-dimensional network structure treated for 48 hours was 472.30 m2/g, 2.5 times that of untreated materials. The original bioactive glass caused the wound surface of 5 cm×5 cm×4 cm to fill the whole wound surface with new tissue on (19.33±0.37) days, the repair of the wound surface required (22.16±0.65)days and (25.5±0.25)days with 24 hours and 48 hours of mineralization,. The bioactive glass group without mineralization could significantly promote wound healing, and the difference was statistically significant (P<0.05). Histological analysis showed that bioactive glass without mineralization could reduce inflammatory response, promote rapid and orderly growth of fibroblasts and capillaries, and complete new tissue structure.
Key words:  bioactive glass    three-dimensional network structure    specific surface area    vitro mineralization    wound repair
               出版日期:  2020-11-25      发布日期:  2021-01-08
ZTFLH:  O613  
通讯作者:  wangjian@suirm.com   
作者简介:  胡方,北京幸福益生再生医学科技有限公司总经理,作为科研带头人和核心科研人员进行生物活性无机非金属材料研究,申请发明专利40余项,国际专利7项。王健,北京幸福益生再生医学科技有限公司研究员,2012年9月—2014年12月于南昌航空大学材料科学与工程学院毕业,主要从事无机非金属材料制作的医用材料器械的研究。发表论文1篇,申请国家发明专利17项,其中授权5项。参与多项国家科技项目,包括国家“十三五”重点研发计划、国家自然科学基金等。
引用本文:    
胡方, 王健, 仇越秀, 郭创洲, 刘明东. 生物活性玻璃不同时间体外矿化对创伤修复的影响[J]. 材料导报, 2020, 34(Z2): 142-146.
HU Fang, WANG Jian, QIU Yuexiu, GUO Chuangzhou, LIU Mingdong. Investigation of the Bioactive Glass on Wound Repair After Mineralization in Vitro for Different Time. Materials Reports, 2020, 34(Z2): 142-146.
链接本文:  
http://www.mater-rep.com/CN/  或          http://www.mater-rep.com/CN/Y2020/V34/IZ2/142
1 李晓琴, 但卫华, 王康建,等,材料导报, 2010, 23(z1),374.
2 Zhao S C, Li L, Wang H, et al.Biomat Erials, 2015, 53, 379.
3 付小兵.中华烧伤杂志,2016, 32(1), 6.
4 方少霞, 郭芬.中国组织工程研究,2016, 20(23), 3491.
5 赵娜如,王迎军,陈晓峰,等.材料导报, 2004, 18(12), 78.
6 杨维虎, 左奕, 张利, 等.无机化学学报, 2009, 25(2), 223.
7 郭明锋, 马杰, 蔡卫林, 等.中华创伤杂志, 2014, 30(1), 85.
8 陈梦越, 李乐之.中华护理杂志, 2016, 51(12), 1483.
9 冯志凯,刘华.中华外科杂志, 2012, 50(4), 368.
10 Yu H F, Peng J L, Xu Y H, et al.ACS Applied Materials & Interfaces, 2016, 8, 703.
11 Ye Y L, Wang S H, Sun J M, et al. Clinical Medicine, 2007, 27(7), 48.
12 廖建贵.小肠粘膜下层冷冻凝胶用于细胞移植支架的研究.硕士学位论文.第三军医大学, 2016.
13 燕晓宇,杨光,成亮,等.中国组织工程研究, 2012, 16(15), 2745.
[1] 马茸茸, 张电, 刘一军, 刘静, 杨晓凤, 李延军, 马爱琼. 多孔氮化硅陶瓷的研究进展及构效关系中的矛盾平衡[J]. 材料导报, 2020, 34(9): 9101-9109.
[2] 张婷, 刘桂华, 陈斌斌, 齐天贵, 周秋生, 彭志宏, 李小斌. 高比表面积氧化铝微粉从碱性废水中除氟[J]. 材料导报, 2020, 34(4): 4020-4024.
[3] 程亮, 赵子龙. 用Ti板制备高比表面积TiO2纳米管的响应面实验设计[J]. 材料导报, 2019, 33(Z2): 365-368.
[4] 高文杰, 杨自春, 李昆锋, 费志方, 陈国兵, 赵爽. 聚酰亚胺纤维增强SiO2气凝胶的制备及表征[J]. 材料导报, 2019, 33(4): 714-718.
[5] 杨果, 马壮, 杨绍斌, 董伟, 沈丁. 低比表面积酚醛树脂硬碳的制备及电化学性能[J]. 材料导报, 2019, 33(22): 3820-3824.
[6] 王勇, 谢帅, 乔丽英, 刘月, 赵川. 二水磷酸氢钙包覆镁粉的组织及其在NaCl溶液中的腐蚀行为[J]. 《材料导报》期刊社, 2018, 32(4): 533-538.
[7] 朱学良, 魏智强, 白军善, 赵文华, 冯旺军, 姜金龙. 碳包覆氧化亚钴纳米颗粒的制备与性能研究[J]. 《材料导报》期刊社, 2018, 32(4): 621-625.
[8] 管庆顺,李建,宋如愿,徐朝阳,吴伟兵,景宜,戴红旗,房桂干. 基于纳米材料的气凝胶制备及应用[J]. 《材料导报》期刊社, 2018, 32(3): 384-390.
[9] 李光大, 张楠, 张开丽, 赵三团, 麻开旺, 许贺龙, 赵威, 谢蟪旭. 含钙铁氧体磁性生物活性玻璃陶瓷热种子的制备与表征[J]. 材料导报, 2018, 32(24): 4211-4216.
[10] 李诗杰, 韩奎华, 韩旭东, 路春美. 马尾藻基高比表面积活性炭的制备及表征[J]. 《材料导报》期刊社, 2017, 31(6): 38-44.
[1] Ninghui LIANG,Peng YANG,Xinrong LIU,Yang ZHONG,Zheqi GUO. A Study on Dynamic Compressive Mechanical Properties of Multi-size Polypropylene Fiber Concrete Under High Strain Rate[J]. Materials Reports, 2018, 32(2): 288 -294 .
[2] WANG Tong, BAO Yan. Advances on Functional Polyacrylate/Inorganic Nanocomposite Latex for Leather Finishing[J]. Materials Reports, 2017, 31(1): 64 -71 .
[3] GUO Hongjian, JIA Junhong, ZHANG Zhenyu, LIANG Bunu, CHEN Wenyuan, LI Bo, WANG Jianyi. Microstructure and Tribological Properties of VN/Ag Films Fabricated by Pulsed Laser Deposition Technique[J]. Materials Reports, 2017, 31(2): 55 -59 .
[4] WANG Wenjin, WANG Keqiang, YE Shenjie, MIAO Weijun, CHEN Zhongren. Effect of Asymmetric Block Copolymer of PI-b-PB on Phase Morphology and Properties of IR/BR Blends[J]. Materials Reports, 2017, 31(2): 96 -100 .
[5] HUANG Dajian, MA Zonghong, MA Chenyang, WANG Xinwei. Preparation and Properties of Gelatin/Chitosan Composite Films Enhanced by Chitin Nanofiber[J]. Materials Reports, 2017, 31(8): 21 -24 .
[6] YUAN Xinjian, LI Ci, WANG Haodong, LIANG Xuebo, ZENG Dingding, XIE Chaojie. Effects of Micro-alloying of Chromium and Vanadium on Microstructure and Mechanical Properties of High Carbon Steel[J]. Materials Reports, 2017, 31(8): 76 -81 .
[7] ZHANG Le, ZHOU Tianyuan, CHEN Hao, YANG Hao, ZHANG Qitu, SONG Bo, WONG Chingping. Advances in Transparent Nd∶YAG Laser Ceramics[J]. Materials Reports, 2017, 31(13): 41 -50 .
[8] YAN Zhilong, LI Yongsheng, HU Kai, ZHOU Xiaorong. Progress of Study on Phase Decomposition of Duplex Stainless Steel[J]. Materials Reports, 2017, 31(15): 75 -80 .
[9] SHI Yu, ZHOU Xianglong, ZHU Ming, GU Yufen, FAN Ding. Effect of Filler Wires on Brazing Interface Microstructure and Mechanical Properties of Al/Cu Dissimilar Metals Welding-Brazing Joint[J]. Materials Reports, 2017, 31(10): 61 .
[10] DONG Fei,YI Youping,HUANG Shiquan,ZHANG Yuxun,. TTT Curves and Quench Sensitivity of 2A14 Aluminum Alloy[J]. Materials Reports, 2017, 31(10): 77 -81 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed