Please wait a minute...
材料导报  2022, Vol. 36 Issue (14): 21020090-7    https://doi.org/10.11896/cldb.21020090
  高分子与聚合物基复合材料 |
以聚醚聚酯丙烯酸酯为交联剂的pH响应性水凝胶纳米微球的制备及药物释放研究
宗丹1, 盛扬1, 李新庆2, 潘艳3, 孙一新1, 邓林红3, Mark Bradley4, 张嵘1
1 常州大学材料科学与工程学院,江苏 常州 213164
2 常州市第二人民医院,江苏 常州 213003
3 常州大学生物医学工程与健康科学研究院,江苏 常州 213164
4 英国爱丁堡大学化学学院,爱丁堡 EH93 JJ
Preparation of pH Responsive Hydrogel Nanospheres Using Polyether Polyester Acrylate Crosslinkers and Their Application in Drug Release
ZONG Dan1, SHENG Yang1, LI Xinqing2, PAN Yan3, SUN Yixin1, DENG Linhong3, Mark Bradley4, ZHANG Rong1
1 School of Materials Science and Engineering, Changzhou University, Changzhou 213164, Jiangsu, China
2 Changzhou Second People's Hospital, Changzhou 213003, Jiangsu, China
3 Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou 213164, Jiangsu, China
4 School of Chemistry, University of Edinburgh, Scotland EH93 JJ, Edinburgh,UK
下载:  全 文 ( PDF ) ( 5665KB ) 
输出:  BibTeX | EndNote (RIS)      
摘要 以聚乙二醇(Mw1000)为引发剂,引发ε-己内酯和L-丙交酯开环聚合,制备聚(己内酯-co-乳酸)-聚乙二醇共聚物二醇(PCLA),用甲基丙烯酸酐封端得到PCLA预聚物单体(PCLAMA),并用红外光谱、核磁氢谱和凝胶渗透色谱对合成产物的组成和结构进行了表征。以PCLAMA为大分子交联剂、N-乙烯基吡咯烷酮和丙烯酸为共聚单体,利用紫外引发聚合制备水凝胶纳米微球(PCLAMA-AP),并对其溶胀、降解、药物释放、细胞毒性等性能进行了分析。结果表明,PCLAMA-AP具有pH响应性,其溶胀率随着pH的增大而变大,其中最佳配比下的纳米微球包载茶多酚的载药量超过57%, 132 h的累计释放率达到30%,PCLAMA-AP与人脂肪干细胞共培养48 h后,细胞存活率高于94%,并且纳米微球能成功进入细胞。因此该水凝胶纳米微球具有作为药物载体的潜力,有可能极大地提高茶多酚的生物利用效率。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
宗丹
盛扬
李新庆
潘艳
孙一新
邓林红
MarkBradley
张嵘
关键词:  聚(己内酯-co-乳酸)  水凝胶纳米微球  药物缓释  生物可降解  茶多酚    
Abstract: Poly(ε-caprolactone-co-lactic acid)-PEG copolymer diols (PCLA) were prepared by ring opening polymerization of ε-caprolactone and L-lactide initiated by polyethylene glycol (Mw 1000). PCLA prepolymer monomers (PCLAMA) were prepared by end capping with methacrylic anhydride. The composition and structure of the synthesized products were characterized by FTIR, 1H-NMR and gel permeation chromatography. Hydrogel nanospheres(PCLAMA-AP)were prepared by UV initiated polymerization, using PCLAMA as a macromolecular cross-linker and N-vinylpyrrolidone and acrylic acid as monomers. Swelling, degradation, drug release and cytotoxicity of the hydrogel nanospheres were analyzed, and results show that the hydrogel nanospheres are pH-responsive and the swelling ratio of the hydrogel increases with pH increasing. The drug loading of tea polyphenol in the hydrogel nanospheres with the best monomer ratio is more than 57%, with cumulative drug release over 132 h reaching almost 30%. The hydrogel nanospheres are non-cytotoxic as evidenced by a cell viability of over 94% after co-culture with human adipose derived stem cells for 48 h, and are also observed to successfully enter the cells. The hydrogel nanospheres thus have potential to be used as drug carriers, which may greatly improve the bioavailability of tea polyphenols.
Key words:  poly(ε-caprolactone-co-L-lactic acid)    hydrogel nanospheres    drug release    biodegradability    tea polyphenols
出版日期:  2022-07-25      发布日期:  2022-07-26
ZTFLH:  TQ460.4  
基金资助: 江苏省六大人才高峰创新团队(SWYY-CXTD- 001);常州市科技局国际合作项目(CZ20190019)
通讯作者:  rzhang@cczu.edu.cn   
作者简介:  宗丹,常州大学在读硕士。2014年9月至2018年6月,在南通大学获得高分子材料科学与工程学士学位。主要致力于茶多酚对脂肪干细胞性能的影响研究及用于药物长期控制释放的可降解聚合物载体的研究。
张嵘,常州大学教授,江苏省特聘教授。2003年博士毕业于英国谢菲尔德大学。主要从事于聚合物微点阵列芯片的制备与应用,(干)细胞的分离、提纯与体外培养相关聚合物的开发,可生物降解聚合物材料的开发,抗/灭菌聚合物材料的研发以及生物医用高分子材料的研发。在Journal of Materials Chemistry A,Materials Science and Enginee-ring: C,Chemical Communications, Biomaterials Science, Biomaterials、《高分子学报》《高分子材料科学与工程》《中国组织工程研究》等期刊发表了近70篇研究论文。
引用本文:    
宗丹, 盛扬, 李新庆, 潘艳, 孙一新, 邓林红, Mark Bradley, 张嵘. 以聚醚聚酯丙烯酸酯为交联剂的pH响应性水凝胶纳米微球的制备及药物释放研究[J]. 材料导报, 2022, 36(14): 21020090-7.
ZONG Dan, SHENG Yang, LI Xinqing, PAN Yan, SUN Yixin, DENG Linhong, Mark Bradley, ZHANG Rong. Preparation of pH Responsive Hydrogel Nanospheres Using Polyether Polyester Acrylate Crosslinkers and Their Application in Drug Release. Materials Reports, 2022, 36(14): 21020090-7.
链接本文:  
http://www.mater-rep.com/CN/10.11896/cldb.21020090  或          http://www.mater-rep.com/CN/Y2022/V36/I14/21020090
1 Hossen M S, Ali M Y, Jahurul M H A, et al. Pharmacological Reports, 2017, 69(6), 1194.
2 Buitimea-Cantúa N E, Gutiérrez-Uribe J A, Serna-Saldívar S O. Journal of Medicinal Food, 2018, 21(2), 188.
3 Gomes A, Oudot C, Macià A, et al. Nutrients, 2019, 11(11), 2634.
4 Zhang L, Chen X, Chen B, et al. Journal of Anhui Agricultural University, 2016, 43(5), 667(in Chinese).
张梁, 陈欣, 陈博, 等. 安徽农业大学学报, 2016, 43(5), 667.
5 Krook M A, Hagerman A E. Food Research International, 2012, 49(1), 112.
6 Uhrich K E, Cannizzaro S M, Langer R S, et al. Chemical Reviews-Columbus, 1999, 99(11), 3181.
7 Hamidi M, Azadi A, Rafiei P. Advanced Drug Delivery Reviews, 2008, 60(15), 1638.
8 Hu Y, Jiang X, Ding Y, et al. Biomaterials, 2003, 24(13), 2395.
9 Zeng Q, Qian Y, Huang Y, et al. Bioactive Materials,2021,6(9),2647.
10 Clasky A J, Watchorn J D, Chen P Z, et al. Acta Biomaterialia, 2021, 122, 1.
11 Woodruff M A, Hutmacher D W. Progress in Polymer Science, 2010, 35(10), 1217.
12 Ponjavic M, Nikolic M S, Nikodinovic-Runic J, et al. Polymer Testing, 2017, 57, 67.
13 Behtaj S, Karamali F, Masaeli E, et al. Biochemical Engineering Journal, 2021, 166, 107846.
14 Saini P, Arora M, Kumar M. Advanced Drug Delivery Reviews, 2016, 107, 47.
15 Basu A, Kunduru K R, Doppalapudi S, et al. Advanced Drug Delivery Reviews, 2016, 107,192.
16 Pauck R G, Reddy B D. Medical Engineering & Physics,2015,37(1),7.
17 Deng X, Zhou S, Li X, et al. Journal of Controlled Release, 2001, 71(2), 165.
18 Hu Y, Xie J, Tong Y W, et al. Journal of Controlled Release, 2007, 118(1), 7.
19 Zuk P A, Zhu M, Ashjian P, et al. Molecular Biology of the Cell, 2002, 13(12), 4279.
20 Zuk P A, Zhu M, Mizuno H, et al. Tissue Engineering,2001,7(2),211.
[1] 黄绪德, 刘欣. 利用维生素C和茶多酚还原氧化石墨烯及其表征[J]. 材料导报, 2021, 35(Z1): 83-86.
[2] 李强, 赵特, 魏磊山, 陈明华, 孙旭东. Cu含量对生物可降解Zn-Cu合金组织和性能的影响[J]. 材料导报, 2021, 35(8): 8088-8092.
[3] 姜英勇, 任亮, 任重, 李文博, 帅嘉欣, 张明耀, 张会轩. 生物可降解PBS聚酯合金的制备与性能调控[J]. 材料导报, 2021, 35(22): 22151-22159.
[4] 赵杰, 钱俊雯, 赵军平. 基于Scopus的生物可降解金属材料文献调研与分析[J]. 材料导报, 2020, 34(Z1): 469-475.
[5] 王异彩, 张甜, 李媛. 光聚合PEXS-A水凝胶材料的合成及包埋活细胞的性能[J]. 材料导报, 2020, 34(2): 2169-2173.
[6] 王雪, 朱昆萌, 彭长鑫, 钟铠, 崔升. 生物可降解多糖气凝胶材料的研究进展[J]. 材料导报, 2019, 33(z1): 476-480.
[7] 高保东, 钟红荣, 吴婷芳, 谭翠, 张岩, 徐水. 丝素/海藻酸钠膜韧性的优化及膜释药机理分析[J]. 《材料导报》期刊社, 2018, 32(7): 1197-1201.
[8] 代晓军, 杨西荣, 王昌, 徐鹏, 赵曦, 于振涛. 生物医用可降解锌基合金的研究进展[J]. 材料导报, 2018, 32(21): 3754-3759.
[9] 郑静, 陈琳, 张欢, 杨永珍, 刘旭光. 有序介孔碳纳米材料的合成及载药系统构建研究进展*[J]. 《材料导报》期刊社, 2017, 31(21): 151-157.
[1] Yanzhen WANG, Mingming CHEN, Chengyang WANG. Preparation and Electrochemical Properties Characterization of High-rate SiO2/C Composite Materials[J]. Materials Reports, 2018, 32(3): 357 -361 .
[2] Yimeng XIA, Shuai WU, Feng TAN, Wei LI, Qingmao WEI, Chungang MIN, Xikun YANG. Effect of Anionic Groups of Cobalt Salt on the Electrocatalytic Activity of Co-N-C Catalysts[J]. Materials Reports, 2018, 32(3): 362 -367 .
[3] Qingshun GUAN,Jian LI,Ruyuan SONG,Zhaoyang XU,Weibing WU,Yi JING,Hongqi DAI,Guigan FANG. A Survey on Preparation and Application of Aerogels Based on Nanomaterials[J]. Materials Reports, 2018, 32(3): 384 -390 .
[4] Lijing YANG,Zhengxian LI,Chunliang HUANG,Pei WANG,Jianhua YAO. Producing Hard Material Coatings by Laser-assisted Cold Spray:a Technological Review[J]. Materials Reports, 2018, 32(3): 412 -417 .
[5] Zhiqiang QIAN,Zhijian WU,Shidong WANG,Huifang ZHANG,Haining LIU,Xiushen YE,Quan LI. Research Progress in Preparation of Superhydrophobic Coatings on Magnesium Alloys and Its Application[J]. Materials Reports, 2018, 32(1): 102 -109 .
[6] Wen XI,Zheng CHEN,Shi HU. Research Progress of Deformation Induced Localized Solid-state Amorphization in Nanocrystalline Materials[J]. Materials Reports, 2018, 32(1): 116 -121 .
[7] Xing LIANG, Guohua GAO, Guangming WU. Research Development of Vanadium Oxide Serving as Cathode Materials for Lithium Ion Batteries[J]. Materials Reports, 2018, 32(1): 12 -33 .
[8] Hao ZHANG,Yongde HUANG,Yue GUO,Qingsong LU. Technological and Process Advances in Robotic Friction Stir Welding[J]. Materials Reports, 2018, 32(1): 128 -134 .
[9] Laima LUO, Mengyao XU, Xiang ZAN, Xiaoyong ZHU, Ping LI, Jigui CHENG, Yucheng WU. Progress in Irradiation Damage of Tungsten and Tungsten AlloysUnder Different Irradiation Particles[J]. Materials Reports, 2018, 32(1): 41 -46 .
[10] Fengsen MA,Yan YU,Jie ZHANG,Haibo CHEN. A State-of-the-art Review of Cytotoxicity Evaluation of Biomaterials[J]. Materials Reports, 2018, 32(1): 76 -85 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed