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材料导报  2018, Vol. 32 Issue (20): 3628-3633    https://doi.org/10.11896/j.issn.1005-023X.2018.20.024
  高分子与聚合物基复合材料 |
双醛羧甲基纤维素-胶原复合止血材料的研制
白忠祥1,2, 但年华1,2, 但卫华1,2, 高然1,2
1 四川大学皮革化学与工程教育部重点实验室,成都 610065;
2 四川大学生物医学工程技术研究中心,成都 610065;
Preparation of Dialdehyde Carboxymethyl Cellulose-Collagen Composite Hemostatic Material
BAI Zhongxiang1,2, DAN Nianhua1,2, DAN Weihua1,2, GAO Ran1,2
1 Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065;
2 Biomedical Engineering Technology Research Center, Sichuan University, Chengdu 610065;
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摘要 以羧甲基纤维素钠(CMC)和胶原(Col)为原料,通过选择性氧化制得双醛羧甲基纤维素钠(DCMC),并对胶原进行交联改性,制得一种新型胶原止血海绵DCMC-Col。运用红外光谱、圆二色谱、差示扫描量热仪、原子力显微镜、扫描电镜对改性后的DCMC-Col复合海绵进行检测。结果表明,改性后胶原的热稳定性得到明显提升,且胶原三股螺旋结构不会遭到破坏。全凝血时间检测表明,当DCMC用量为15%时,海绵的止血性能最优。吸水率、溶血率和MTT实验结果表明,DCMC-Col复合海绵的各项指标均满足止血材料的要求,且细胞相容性良好。因此,DCMC-Col复合海绵是一种具有潜在医学应用价值的新型止血材料。
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白忠祥
但年华
但卫华
高然
关键词:  羧甲基纤维素钠  Ⅰ型胶原  改性  止血材料    
Abstract: Dialdehyde carboxymethyl cellulose sodium (DCMC) was selectively oxidized utilizing sodium carboxymethyl cellulose (CMC) and collagen (Col) as raw materials, and the collagen was cross-linked and modified to obtain a new type collagen hemostatic sponge DCMC-Col. The modified DCMC-Col composites were evaluated by infrared spectroscopy, circular dichroism, differential scanning calorimetry, atomic force microscopy and scanning electron microscopy. The results indicated that the thermal stability of the modified collagen was significantly improved, and the triple helix structure of collagen was not destroyed. The total clotting time test confirmed that the hemostatic property of the sponge was optimal when the dosage of DCMC was 15%. The results of water absorption, hemolysis and MTT test results demonstrated that the properties of DCMC-Col could meet the requirements of hemosta-tic materials and it had a good biocompatibility. Therefore, DCMC-Col composite sponge is a new type of hemostatic materials with potential medical applications value.
Key words:  carboxymethyl cellulose    type Ⅰ collagen    modification    hemostatic materials
               出版日期:  2018-10-25      发布日期:  2018-11-22
ZTFLH:  O636  
基金资助: 国家自然科学基金(51473001)
作者简介:  白忠祥:男,1994年生,硕士研究生,研究方向为胶原基生物医学材料 E-mail:17380140172@163.com 但卫华:通信作者,男,1956年生,博士,教授,博士研究生导师,研究方向为生物质医学材料及功能皮革 E-mail:dwh5607@263.net
引用本文:    
白忠祥, 但年华, 但卫华, 高然. 双醛羧甲基纤维素-胶原复合止血材料的研制[J]. 材料导报, 2018, 32(20): 3628-3633.
BAI Zhongxiang, DAN Nianhua, DAN Weihua, GAO Ran. Preparation of Dialdehyde Carboxymethyl Cellulose-Collagen Composite Hemostatic Material. Materials Reports, 2018, 32(20): 3628-3633.
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http://www.mater-rep.com/CN/10.11896/j.issn.1005-023X.2018.20.024  或          http://www.mater-rep.com/CN/Y2018/V32/I20/3628
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