可激活荧光-磁共振双模态纳米材料的制备与性能

材料导报

2020, Vol. 34

Issue (Z1): 97-102

无机非金属及其复合材料

可激活荧光-磁共振双模态纳米材料的制备与性能

郭潇¹, 周玉洁¹, 高静茹¹, 余薇¹, 许翠¹, 韩翠平^1,2

1 徐州医科大学医学影像学院,徐州 221004;
2 徐州医科大学附属医院影像科,徐州 221000

Preparation and Properties of Activable Fluorescence-Magnetic ResonanceDual-mode Nanoprobe

GUO Xiao¹, ZHOU Yujie¹, GAO Jingru¹, YU Wei¹, XU Cui¹, HAN Cuiping^1,2

1 School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China;
2 Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China

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摘要本工作以二水合柠檬酸钠和尿素为原料,运用无溶剂法在200 ℃的高温条件下反应1 h,得到量子产率为93%的荧光碳点。在此基础上,利用聚(丙烯胺盐酸盐)(PAH)为耦合剂,将制备的CDs与MnO₂纳米片通过静电组装结合起来,构建谷胱甘肽(GSH)可激活荧光-磁共振双模态纳米材料MnO₂-CDs,并对MnO₂-CDs的制备进行优化,提高成像质量。考察MnO₂-CDs的荧光和磁共振信号的可激活性,在GSH存在下,荧光得以恢复,T1加权信号增强,T1弛豫效率r₁为7.01 mmol^-1·L·s^-1。材料浓度高达2 mg/mL时,细胞的存活率仍在85%以上。MnO₂-CDs与HO-8910卵巢癌细胞孵育后,细胞呈现明亮的荧光和T1加权信号,而Ea.hy926正常细胞仅显示微弱的低信号。本工作所研制GSH激活的荧光-磁共振双模态MnO₂-CDs不仅具有较高的量子产率和弛豫效率的成像性能要求,同时具有较低的细胞毒性。此外,这些纳米粒子通过肿瘤细胞内高表达的GSH激活信号,可以同时利用荧光和磁共振成像技术对癌细胞进行检测,将大大增加信噪比,提高成像灵敏度。

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	郭潇
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	韩翠平

关键词: 可激活荧光碳点二氧化锰纳米片磁共振成像双模态

Abstract: In this work, fluorescent carbon dots with the quantum yield of 93% were prepared using a solvent-free method by carbonization of sodium citrate and urea at 200 ℃ for 1 h. The prepared CDs and MnO₂ nanosheets were electrostatically assembled to construct activated fluorescence-magnetic resonance dual-modal MnO₂-CDs using poly (allylamine hydrochloride) (PAH) as coupling agent. The preparation of MnO₂-CDs was optimized to improve the imaging performance. The activation of fluorescence and magnetic resonance signals of MnO₂-CDs was investigated. In the presence of GSH, the fluorescence was restored, and the T1-weighted signal was enhanced with a relaxation rate of 7.01 mmol^-1·L·s^-1. When the concentration of the material was as high as 2 mg/mL, the survival rate of the cells was still above 85%. The HO-8910 ovarian cancer cells showed bright fluorescence and T1-weighted signals after incubation with MnO₂-CDs, while Ea.hy926 normal cells showed only weak signals.The fluorescence and magnetic resonance bimodal GSH-activatable nanoprobe MnO₂-CDs not only had high quantum yield and T1 relaxation efficiency, but also had low cytotoxicity. Moreover, these nanoparticles could allow for the detection of cancer cells with both fluorescence and MRI imaging techniques through the GSH activated signals would greatly increase the target-to-background signal ratio for higher imaging sensitivity.

Key words: activatable fluorescent carbon dots manganese dioxide nanosheets magnetic resonance imaging dual-mode

发布日期: 2020-07-01

ZTFLH:

R-331

基金资助: 国家自然科学基金(81671744);中国博士后基金(2016M591923;2017T100405);江苏省“科教强卫工程”青年医学人才(QNRC2016782);江苏省“六大人才高峰”(WSW-051);江苏省高等学校大学生创新创业训练计划项目(201710313004Z)

作者简介: 郭潇,徐州医科大学在读硕士。2014年9月至2019年6月,在徐州医科大学获得医学影像学学士学位,2019届徐州医科大学优秀毕业生,毕业后继续在徐州医科大学攻读影像医学与核医学专业研究生。以第一作者发表中文核心期刊1篇,以第三作者发表SCI一篇,以第三作者发表国家级期刊1篇。担任2017年大学生创新创业训练计划省级重点项目《双靶点可激活探针的卵巢癌细胞荧光-MRI分子成像研究》第一负责人,并顺利结题;韩翠平,博士,硕士研究生导师,江苏省“333工程”第三层次人才、江苏省“六大人才高峰”高层次人才(C类)、江苏省高校“青蓝工程”优秀青年骨干教师,江苏省“科教强卫工程”青年人才,江苏省医学会数字医学分会青年委员。主要从事纳米生物医学技术与分子影像的研究,主持国家自然科学基金面上项目、国家自然科学基金青年项目、江苏省自然科学基金项目、中国博士后基金以及江苏省博士后基金等9项科研课题。以第一作者或通讯作者发表SCI研究论文19篇。指导国家级大学生创新训练计划重点项目2项、一般项目2项。协助指导硕士研究生10名。

引用本文:

郭潇, 周玉洁, 高静茹, 余薇, 许翠, 韩翠平. 可激活荧光-磁共振双模态纳米材料的制备与性能[J]. 材料导报, 2020, 34(Z1): 97-102.
GUO Xiao, ZHOU Yujie, GAO Jingru, YU Wei, XU Cui, HAN Cuiping. Preparation and Properties of Activable Fluorescence-Magnetic ResonanceDual-mode Nanoprobe. Materials Reports, 2020, 34(Z1): 97-102.

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http://www.mater-rep.com/CN/ 或 http://www.mater-rep.com/CN/Y2020/V34/IZ1/97

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