Abstract: Based on good performance in the application of biomaterial, silk fibroin, gelatin and chitosan were used to prepare a new type of tissue engineering material via twice lyophilization. The prepared material possesses highly loose and inter-connective microstructure. In the experiment, the physical and chemical properties of silk fibroin/gelatin/chitosan composite scaffold material were studied first; then the morphology of this material was investigated; finally, the cytotoxicity and biocompatibility of this mate-rial were evaluated. The experimental results showed that the porosity of the scaffold material was greater than (53.52±1.16)%, and the water absorption of the scaffold material was larger than (89.36±0.43)%. In addition, the optimal value for mechanical elongation at break of silk fibroin/gelatin/chitosan composite scaffold material is (11.5±1.05)% when the pretreatment temperature reaches -20 ℃, and the ratio of silk fibroin/gelatin/chitosan was 1∶1∶1.5 with crosslinker of glutaraldehyde. Infrared spectrum and X-ray diffraction revealed the interaction among the chemical groups of each component within the prepared material and the crystalline state change of this material respectively. The engraftment of silk fibroin/gelatin/chitosan composite scaffold material in mice demonstrated that the scaffold material is non-toxic, biocompatible, and therefore can be used as a comprehensive and excellent tissue engineering material.
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2018, Vol. 32 
