Biocompatibility Assessment and Chondrogenic Differentiation of Poly-L-lactide-based Nanofibrous Scaffolds
HONG Yazhen1,2, YANG Dingzhu1,2
1 College of Chemical Engineering, Huaqiao University, Xiamen 361021; 2 Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021
Abstract: Initially, we prepared poly-L-lactide (PLLA)-based porous scaffolds, and then the biosafety, as well as chondrogenic differentiation potential of the same, were evaluated. The biocompatibility of nanofibrous scaffolds was assessed by using various tests in different models. At first, the cytotoxicity was measured using L929 as cell model using Alamar Blue assay. In addition, the acute systemic toxicity was measured by injecting scaffold in the form of extracts into KM mice. Furthermore, hemolysis test of the scaffold was investigated using fresh blood drawn from rabbit as blood model. The scaffolds resulted in low cytotoxicity, no acute toxicity in mice, and highly compatible with the blood. Eventually, the chondrogenic differentiation potential of C5.18 cells on the designed scaffolds with diversified topology was determined. In addition, dexamethasone (DEX) was used for the enhancement of differentiation process as it favors the differentiation of C5.18 cells at 10-6 mol/L of DEX. The degree of cartilage differentiation and the production of glycosaminoglycans and collagen protein was augmented with the increase in induction time. These nanofibrous scaffolds can serve as a prospect material for cartilage tissue construction with a unique advantage in promoting the differentiation of chondrogenesis.
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2018, Vol. 32 
