Preparation and Antifungal Properties of Self-assembled Amphiphilic Chitosan Nanocapsules
HE Zuyu1, XIE Jianghui1, LI Puwang1, QU Yunhui2, YANG Ziming1, YU Lijuan2, WANG Chao1, LIU Yunhao1, YAO Quansheng1, ZHOU Chuang1
1 Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crop Research Institute of China Academy of Tropical Agricultural Sciences, Zhanjiang 524091,China 2 Agricultural Product Processing Research Institute of Yunnan Academy of Agricultural Sciences, Kunming 650000, China
Abstract: In this paper, eleostearic acid (EA) and N-acetyl-L-cysteine (NAC) were grafted to the chain of chitosan (CS) to prepare an amphiphilic chitosan derivative (CS-g-EA-NAC), and the chemical structure of CS-g-EA-NAC was characterized by Fourier transform infrared (FT-IR) and 1H nuclear magnetic resonance (1H NMR). Then, CS-g-EA-NAC based nanoparticles were prepared by ultrasonic self-assembly method, and the spinosad (SSD) loaded nanocapsules (SSD@CS-g-EA-NAC) were spherical in shape with a uniform particle size distribution, and the average particle size was about 520 nm. In vitro release revealed that the SSD@CS-g-EA-NAC nanocapsules exhibited a sustained and pH-responsive drug release property. Finally, the antifungal properties of SSD@CS-g-EA-NAC and SSD against Fusarium oxysporum were determined by the growth rate method. The results showed that the half effective concentration (EC50) of SSD@CS-g-EA-NAC and SSD against Fusarium oxysporum was were 29.05 μg/mL and 42.05 μg/mL, respectively, indicating that the virulence of SSD@CS-g-EA-NAC is higher than that of free SSD, and the conjugate CS-g-EA-NAC is conducive to improve the virulence of SSD. This research can provide a theoretical basis for the construction of chitosan-based drug delivery system.
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2020, Vol. 34 
