Preparation of Coacervate Microdroplets Based on Carboxymethyl Chitosan and Carboxyl Dextran and Its Cytotoxicity Towards Yeast
HU Hanjiao1, LIANG Xingtang2, WANG Shuangshuang2, ZHANG Xia1, YIN Yanzhen1,2, YIN Xuebin3
1 School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China 2 College of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou 535011, China 3 Advanced Laboratory for Functional Agriculture, Suzhou Institute for Advanced Study, University of Science and Technology of China, Suzhou 215123, China
Abstract: Coacervate microdroplets can form an artificial cell wall on the cell surface via self-assembly, which can protect the cell and decrease the damage caused by external stimulation and chemical environment. Natural polymers with advantages such as good biocompatibility and biodegradability, can be employed to prepare coacervate microdroplets with stable structure. It is of great significance to explore the preparation methods of coacervate microdroplets and their application in the cell protection. In this paper, chitosan and dextran were used to prepare the basic element materials of coacervate microdroplets, carboxymethyl chitosan (CMCS) and carboxyl dextran (DEX-COOH), which were employed to construct the coacervate microdroplets. Some techniques such as Fourier Transform Infrared Spectrometer (FTIR), UV-visable spectrophotometry (UV-vis), fluorescent inverted microscope (FIM), confocal laser scanning microscope (CLSM) and dynamic light scattering (DLS) were used to characterize the structure of coacervate microdroplets. The results showed that the stability of the coacervate microdroplets prepard under the condition that the mass concentration ratio of CMCS and DEX-COOH was 1∶2. Without cytotoxicity to saccharomyces cerevisiae, the prepared coacervate microdroplets present potential applications in the improvement of probiotics resistance to external stimulation.
胡汉娇, 梁兴唐, 汪双双, 张霞, 尹艳镇, 尹雪斌. 羧甲基壳聚糖-羧基葡聚糖凝聚微滴的制备及其酵母细胞毒性[J]. 材料导报, 2020, 34(Z2): 496-500.
HU Hanjiao, LIANG Xingtang, WANG Shuangshuang, ZHANG Xia, YIN Yanzhen, YIN Xuebin. Preparation of Coacervate Microdroplets Based on Carboxymethyl Chitosan and Carboxyl Dextran and Its Cytotoxicity Towards Yeast. Materials Reports, 2020, 34(Z2): 496-500.
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2020, Vol. 34 
