Abstract: Thrombosis and infections are the two major clinical complications that lead to the failure of blood-contacting biomaterials, such as in vitro blood circulation and indwelling devices. Grafting of bio-functional molecules is one of the important methods for surface modification of biomaterials. However, existing methods to solve these two complications are often based on complex modification strategies and a series of adverse reactions, for example, the commonly used silver-containing antibacterial strategy exhibits undesired hemocompatibility. Therefore, improving the antibacterial and anticoagulant properties of blood-contacting materials by simply grafting biomolecules is challenging and significant. In this study, poly(allylamine) (PAa) modified poly(dopamine) (PDA) through Schiff alkali reaction and Michael addition in alkaline condition to construct a rich-amine coating (PADA). HA was covalently immobilized to PADA based on amine coupling. The functional coating (HA-PADA) obtained both anticoagulant and antibacterial properties due to the water binding capacity and antifouling property of HA. The successful preparation of the HA-PADA coating was confirmed by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results of the adhesion and activation of fibrinogen and platelets and the antibacterial test on the surfaces confirmed that the HA-PADA coatings can significantly inhibit coagulation and bacterial adhesion. We anticipate that the dual-functional HA-PADA coating strategy with stable and ultra-high HA density would be a milestone in the development of surface engineering, especially to that of blood-contacting biomedical devices.
马文梅, 黄楠, 熊开琴. 基于共价固定高密度透明质酸构建具有抗菌抗凝血双功能的表面[J]. 材料导报, 2020, 34(8): 8165-8171.
MA Wenmei, HUANG Nan, XIONG Kaiqin. Construction of Surface with Anticoagulant and Antibacterial Functions via a Covalent Immobilization of Hyaluronic Acid with High Density. Materials Reports, 2020, 34(8): 8165-8171.
1 Vogler E A, Siedlecki C A. Biomaterials, 2009, 30(10), 1857. 2 Moellering R C. Journal of Antimicrobial Chemotherapy, 2012, 67(1), 4. 3 Benedetta A, Sepideh B N, Christophe C,et al. Lancet, 2011, 377(9761), 228. 4 Aw J, Widjaja F, Ding Y. et al. Chemical Communications, 2017, 53(23), 3330. 5 Ji H, Sun H, Qu X. Advanced Drug Delivery Reviews, 2016, 105, 176. 6 Pai M P,Mercier R C,Koster S A. Annals of Pharmacotherapy, 2006, 40(2), 224. 7 Matthijs D H, Mouton J W, Anker J N, et al. Clinical Pharmacokinetics, 2004, 43(7), 417. 8 Majumdar P,Webster D C. Macromolecules, 2005, 38(14),5857. 9 Vermette P, Meagher L,et al. Colloids & Surfaces B Biointerfaces, 2003, 28(2), 153. 10 Kim Y H, Dong K H, Park K D, et al. Biomaterials, 2003, 24(13), 2213. 11 Zheng Z, Timothy C, Shengfu C, et al. Langmuir, 2006, 22(24), 10072. 12 Sibarani J, Takai M, Ishihara K. Colloids & Surfaces B Biointerfaces, 2007, 54(1), 88. 13 Xu Q W, Xiao S Y, et al. Chinese Tissue Engineering Research, 2007, 11(13), 2415(in Chinese). 许乾慰, 肖世英, 等.中国组织工程研究, 2007, 11(13), 2415 14 Dang Y P, Cai S X, Ma K W. Chinese Journal of Medical Physics, 2007, 24(6), 435(in Chinese). 党英萍, 蔡绍皙, 麻开旺. 中国医学物理学杂志, 2007, 24(6), 435. 15 Tang C, Wang G, Cao Y, et al. Applied Surface Science, 2008, 255(2), 315. 16 Hu C, Liu S, Li B, et al. Advanced Healthcare Materials, 2013, 2(10), 1314. 17 Chen H T, Lin H L, Chen I G. et al. ACS Applied Materials & Interfaces, 2015, 7(18), 9479. 18 Hui Q, Sun C, Chao H, et al. Journal of Membrane Science, 2014, 468(20), 172. 19 Chou Y N, Venault A, Cho C H, et al. Langmuir the ACS Journal of Surfaces & Colloids, 2017, 33(38), 7b02164. 20 Yang Z, Tu Q, Wang J, et al. Biomaterials, 2012, 33(28), 6615. 21 Taolei S, Hong T, Dong H, et al. Small, 2010, 1(10), 959. 22 Yang Z, Yang Y, Xiong K, et al. Biomaterials, 2015, 63,80. 23 Prime K L, Whitesides G M. Science, 1991, 252(5009), 1164. 24 Liu X, Huang R, Su R, et al. ACS Applied Materials & Interfaces, 2014, 6(15), 13034. 25 Stella B, Maria Pilar A S, Finlay J A, et al. Langmuir the ACS Journal of Surfaces & Colloids, 2013, 29(12), 4039. 26 Kafedjiiski K, Jetti R K R, Föger F, et al. International Journal of Pharmaceutics, 2007, 343(1), 48. 27 Barbucci R, Lamponi S, Magnani A, et al. Journal of Thrombosis & Thrombolysis, 1998, 6(2), 109. 28 Wu F, Li J, Zhang K, et al. ACS Applied Materials & Interfaces, 2016, 8(1), 109. 29 Xue P, Li Q, Li Y, et al. ACS Applied Materials & Interfaces, 2017, 9(39), 33632. 30 Cho Y, Si Y S, Bang J P, et al. Macromolecular Research, 2017, 25(8), 1. 31 Guicai L, Ping Y, Yuzhen L, et al. Biomacromolecules, 2011, 12(4), 1155. 32 Ritchie J L, H Dennis A, Patrick A, et al. British Journal of Haematology, 2015, 116(4), 892. 33 Li Gang, Fu Tao. Journal of Chongqing University of Technology (Natural Science),2019,33(10),73(in Chinese). 李纲, 付涛. 重庆理工大学学报(自然科学),2019,33(10),73. 34 Li X, Hua Q, Peng G, et al. NPG Asia Materials, 2018, 10(10), 38. 35 Sato M, Ohashi T. Biorheology, 2005, 42(6), 421. 36 Borah R, Kumar A, Das M K, et al. RSC Advances, 2015, 5(60), 48971. 37 Yu X L, Wang C D, Li B L, et al. Biomedical Engineering Research, 2005, 24(1), 61(in Chinese). 于学丽, 王传栋, 李保陆, 等. 生物医学工程研究, 2005, 24(1), 61. 38 Wootton D M, Ku D N. Annual Review of Biomedical Engineering, 1999, 1(1), 299. 39 Wu K K. Journal of Internal Medicine, 1996, 239(1), 17. 40 Shi Q, Luan S F, Jin J, et al. China Material Progress, 2014, 33(4), 212(in Chinese). 石强, 栾世方, 金晶,等. 中国材料进展, 2014, 33(4), 212. 41 Zeng Fanpeng, Hu Guang, Luo Limei, et al. Journal of Chongqing University of Technology (Natural Science),2019,33(11),185(in Chinese). 曾凡鹏, 胡光, 罗丽梅, 等. 重庆理工大学学报(自然科学), 2019,33(11),185. 42 Michanetzis G P A, Katsala N, Missirlis Y F. Biomaterials, 2003, 24(4), 677. 43 Wendel H P, Weber N, Ziemer G. Immunopharmacology, 1999, 43(2-3), 149. 44 Hamerli P, Weigel T, Groth T, et al. Biomaterials, 2003, 24(22), 3989. 45 Liu L, Freedman J, Hornstein A,et al. British Journal of Haematology, 2015, 92(2), 458. 46 Shi H C, Yin J H, et al. Chinese Polymer Bulletin, 2016 (9), 196(in Chinese). 石恒冲, 殷敬华,等. 高分子通报, 2016 (9), 196. 47 Matthew N, Miljan K, Mostafa N, et al. Journal of Physical Chemistry B, 2013, 117(47), 14697. 48 Bechinger B, Gorr S U. Journal of Dental Research, 2017, 96(3), 254.