Abstract: To reveal the mechanisms of resistance to cytospora chrysosperma of biocontrol strain F57 (Streptomyces sioyaensis), the effects of fermentation broth of Streptomyces on the induced resistance was studied, and the optimal fermentation medium and fermentation conditions of strain F57 were defined meanwhile, in this study. The optimum fermentation conditions in the Shaker with different nutrient sources were selected through designing orthogonal experiment by using the Valsasordidaas indicators. The annual Populuspyramidalis cv. Huangkuai cuttings were inoculated with Valsasordida by the pinprick method, and the activity of the PAL, the changes of the contents of flavonoids, total phenol, proline and lignin in the leaves were determined by colorimetry. The results showed that the best carbon source is the soluble starch, the best organic nitrogen source is peanut powder and ammonium sulfate is the best inorganic nitrogen source. The optimum medium formula for biocontrol Streptomyces strain F57 was expressed as the following: 70 g soluble starch, 25 g peanut powder, 2 g yester powder, 3 g NH4NO4, 2.7 g CaCO3, 2.7 g NaCl, 1 000 mL water, at pH 7.2. The optimum conditions for fermentation in shake-flask were defined as follows: the fermentation temperature was 28 ℃; initial pH at 7.0; the fermentation time was 5 days; five-day old strains cultivated on medium for seed broth preparation; the volume of broth in a 250 mL flask was 40 mL and inoculation amount was 3%. The results of disease resistance mechanism showed that: after treated with fermentation broth, the contents of proline and lignin were increased in poplar and played a role in protective and therapeutic on the pathogen of poplar canker, which indicated that the fermentation broth has a therapeutic effect on the pathogen of poplar canker. The results on the metabolic pathway of phenylpropanoid metabolism in plants showed that spraying fermentation broth could increase the activity of PAL and cause the contents of flavonoid and total phenol increase, thus enhancing the disease resistance in poplar. In conclusion the fermentation broth of Streptomyces could inhibit pathogenic bacteria. Spraying the fermentation after bacteria invasion could increase the activity of PAL and the contents of proline, lignin, flavonoid and total phenol, thus enhancing the resistance in poplar.
作者简介: † These authors contributed equally to this work. 郑甜甜,2017毕业于辽宁大学,获得微生物学硕士学位,中国农业科学院烟草研究所工作,主要从事烟草赤星病的抗病机制。发表学术论文6篇,授权国家发明专利1项。 隋妍,2017年毕业于辽宁大学生物技术专业,获理学学士学位,现于中国科学院武汉病毒研究所微生物专业攻读硕士学位。曾发表学术论文1篇,授权国家发明专利1项。 李立梅,吉林省林业科学研究院,副研究员,硕士研究生导师。2010年毕业于沈阳农业大学植物病理专业,获农学博士学位。主要研究方向为森林病理学和生物防治,主持和骨干参加科研项目10余项,获省科技进步三等奖2项。在国内外学术期刊上发表论文20余篇,授权国家发明专利2项,实用新型专利10余项。
引用本文:
郑甜甜, 隋妍, 陈越渠, 朱春玉, 毛赫, 李立梅. 盐屋链霉菌发酵条件优化及抗病机理研究[J]. 材料导报, 2019, 33(Z2): 559-567.
ZHENG Tiantian, SUI Yan, CHEN Yuequ, ZHU Chunyu, MAO He, LI Limei. Optimization of Fermentation Conditions and Study on Resistance Mechanism ofStreptomyces Sioyaensis. Materials Reports, 2019, 33(Z2): 559-567.
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2019, Vol. 33 
