二肉豆蔻酰磷脂酰胆碱修饰的氧化铁纳米粒子在PC-12细胞内的分布

doi:10.11896/cldb.201906025

材料导报

2019, Vol. 33

Issue (6): 1047-1051 https://doi.org/10.11896/cldb.201906025

高分子与聚合物基复合材料

二肉豆蔻酰磷脂酰胆碱修饰的氧化铁纳米粒子在PC-12细胞内的分布

韩贵华¹, 张宝林¹, 苏礼超¹, 黄银平¹, 范子梁², 赵应征²

1 桂林理工大学材料科学与工程学院,广西有色金属及特色材料加工省部共建国家重点实验室培育基地,桂林 541004
2 温州医科大学药学院,温州 325035

Cellular Distribution of 1,2-dimyristoyl-sn-glycero-3-phosphocholine Modified Iron Oxide Nanoparticles

HAN Guihua¹, ZHANG Baolin¹, SU Lichao¹, HUANG Yinping¹, FAN Ziliang², ZHAO Yingzheng²

1 State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, College of Materials Science and Engineering, Guilin University of Technology, Guilin 541004
2 School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035

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摘要有机物包覆的超顺磁性氧化铁纳米粒子(Superparamagnetic iron oxide nanoparticles,SPIONs)因具有良好的水溶性和生物相容性而被越来越多地用作生物医学研究的工具。采用简便的高温热分解法合成聚乙二醇(Polyethylene glycol,PEG)和聚乙烯亚胺(Polyethyleneimine,PEI)修饰的超顺磁性氧化铁纳米粒子(PEG/PEI-SPIONs),再在其表面通过氢键相互作用接枝二肉豆蔻酰磷脂酰胆碱(1, 2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC),成功制备了DMPC-SPIONs。由热重结果分析可知DMPC的接枝率约为31.7%(质量分数)。将PEG/PEI-SPIONs和DMPC-SPIONs分别与PC-12细胞孵化,对孵化后的细胞进行表征和分析发现,大量的DMPC-SPIONs进入了细胞,而PEG/PEI-SPIONs进入细胞内的量较少。这表明DMPC对于氧化铁纳米粒子进入细胞起到关键作用。通过透射电镜观察与DMPC-SPIONs孵化的PC-12细胞发现,氧化铁纳米粒子密集分布于溶酶体、线粒体、内质网和细胞核膜表面,另外,也有一部分DMPC-SPIONs分布在细胞膜上的纤毛附近,并观察到细胞膜对纳米粒子的内吞现象。DMPC-SPIONs良好的膜透过性及在胞内细胞器的密集分布使其在磁热疗、磁共振成像、药物输运等生物医学领域具有广阔的应用前景。

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	韩贵华
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	赵应征

关键词: 超顺磁性氧化铁纳米粒子修饰二肉豆蔻酰磷脂酰胆碱细胞内分布透射电镜形貌

Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) coated with organics have been increasingly used in biomedical research in recent years because of their good aqueous dispersibility and biocompatibility, which is essential for nanoparticles to enter cells. SPIONs coated with polyethylene glycol (PEG) and polyethyleneimine (PEI) (PEG/PEI-SPIONs) were synthesized by high temperature thermal decomposition method, then PEG/PEI-SPIONs were modified with 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) by hydrogen bonding interactions to obtain DMPC-SPIONs, and thermogravimetric analysis showed that the mass fraction of DMPC on the surface of SPIONs was 31.7wt%. The research on the uptake of PEG/PEI-SPIONs and DMPC-SPIONs by PC-12 cells showed that much more DMPC-SPIONs entered the cells, whereas relatively fewer PEG/PEI-SPIONs were observed in the cells. The results showed that DMPC played an important role in increasing the entry of SPIONs into cells. DMPC-SPIONs were densely distributed in the lysosomes, mitochondria, endoplasmic reticulum and around the nuclei in PC-12 cells. Some DMPC-SPIONs remained in the vicinity of cilia. The endocytosis process of DMPC-SPIONs was observed. DMPC-SPIONs have great potential to be used as hyperthermia agents, MRI contrast agents and drug transportation carriers.

Key words: superparamagnetic iron oxide nanoparticles (SPIONs) modification 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) cellular distribution TEM morphology

发布日期: 2019-04-03

ZTFLH:

TB332

基金资助: 国家自然科学基金(51562007)

作者简介: 韩贵华,桂林理工大学材料科学与工程学院在读硕士研究生(2016—2019),主要从事超顺磁性氧化铁纳米粒子的合成及大鼠脑内分布应用的研究。张宝林,桂林理工大学材料科学与工程学院,教授。

引用本文:

韩贵华, 张宝林, 苏礼超, 黄银平, 范子梁, 赵应征. 二肉豆蔻酰磷脂酰胆碱修饰的氧化铁纳米粒子在PC-12细胞内的分布[J]. 材料导报, 2019, 33(6): 1047-1051.
HAN Guihua, ZHANG Baolin, SU Lichao, HUANG Yinping, FAN Ziliang, ZHAO Yingzheng. Cellular Distribution of 1,2-dimyristoyl-sn-glycero-3-phosphocholine Modified Iron Oxide Nanoparticles. Materials Reports, 2019, 33(6): 1047-1051.

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http://www.mater-rep.com/CN/10.11896/cldb.201906025 或 http://www.mater-rep.com/CN/Y2019/V33/I6/1047

1 Wu S J, Liu Y G, Wang S B. Materials Review A:Review Papers,2015,29(7),89(in Chinese).
吴慎剑, 刘源岗, 王士斌. 材料导报:综述篇,2015,29(7),89.
2 Ji X H, Liu J X, Tang J G, et al. Materials Review B:Research Papers,2016,30(6),22(in Chinese).
纪小红,刘继宪, 唐建国, 等. 材料导报:研究篇,2016,30(6), 22.
3 Song M M, Xu H L, Liang J X, et al. Materials Science and Engineering C,2017,77,904.
4 Philip Grossen, Gabriela Québatte, Dominik Witzigmann, et al. Journal of Nanomaterials,2016,2016,1.
5 Luo B H, Wang S Q, Rao R, et al. Nanoscale Research Letters,2016,11(1),227.
6 Julien Nicolas, Simona Mura, Davide Bramblla, et al. Chemical Society Reviews,2013,42(3),1147.
7 Geng X D, Shan X H, Xiong F, et al. Shandong Medical Journal,2012,52(11),1(in Chinese).
耿兴东, 单秀红, 熊非, 等. 山东医药,2012,52(11),1.
8 Gallo J, Long N J, Aboagye E O. Chemical Society Reviews,2013,42(19),7816.
9 Zhou L J, Liu J P, Xiong F, et al. Journal of China Medical University,2013,44(4),316(in Chinese).
周丽君, 刘建平, 熊非, 等. 中国医科大学学报,2013,44(4),316.
10 Liu D F, Ding Q, Wen S, et al. In: Conference Record of the 28th Annual Conference of China Chemical Society, Chengdu, 2012,pp.2(in Chinese).
柳东芳, 丁琪, 文颂, 等.中国化学会第28届学术年会,成都,2012,pp.2.
11 Michael Nagel, Stephan Brauckmann, Franzeska Moegle-Hofacker, et al. Biochimica et Biophysica Acta (BBA) - Biomembranes,2015,1848(10),2271.
12 Nazli Ezer, Ipek Sahin, Nadide Kazanci. Vibrational Spectroscopy,2017,89,1.
13 Gianni Ciofani, Giada Graziana Genchi, Barbara Mazzolai, et al. Biochimica et Biophysica Acta (BBA) - Biomembranes,2014,1840,495.
14 Jeong Ah Kim, Nohyun Lee, Byung Hyo Kim, et al. Biomaterials,2011,32,2871.
15 Zhao F Y, Zhang B L, Wang J, et al. Journal of Nanoscience and Nanotechnology,2013,13(10),6793.
16 Xu P F, Shen Z W, Zhang B L, et al. Applied Surface Science,2016,389,560.
17 Zhang S X, Niu H G, Zhang Y Y, et al. Journal of Chromatography A,2012,1238,38.
18 Noga Gal, Andrea Lassenberger, Laia Herrero-Nogareda, et al. ACS Biomaterial Science and Engineering,2017,3,249.
19 Xu S, Olenyuk B Z, Okamoto C T, et al. Advanced Drug Delivery Reviews, 2013,65(1),121.
20 Li Y, Yuan B, Yang K, et al. Nanotechnology, 2017,28(8),85.
21 Zhang J L, Cai Q, Zhao J P. Ultrastructure of the nervous system,Peking Union Medical College Press,China,2011(in Chinese).
张进禄, 蔡青, 赵君朋. 神经系统超微结构,中国协和医科大学出版社,2011.
22 Costanzo Manuela,Scolaro Lucia, Berlier Gloria, et al. International Journal of Pharmaceutics,2016,508(1-2), 83.
23 Zhang X D, Zhang H Q, Liang X,et al. Molecular Pharmaceutics,2016,13,2578.
24 Yang G, Ma W Q, Zhang B L, et al. Materials Science and Engineering C,2016,62,384.
25 Nohyun Lee, Dongwon Yoo, Daishun Ling, et al. Chemical Reviews, 2015,115(19),10637.
26 Reuben Hudson. RSC Advances,2016,6(5),4262.

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