Cellular Distribution of 1,2-dimyristoyl-sn-glycero-3-phosphocholine Modified Iron Oxide Nanoparticles
HAN Guihua1, ZHANG Baolin1, SU Lichao1, HUANG Yinping1, FAN Ziliang2, ZHAO Yingzheng2
1 State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, College of Materials Science and Engineering, Guilin University of Technology, Guilin 541004 2 School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035
Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) coated with organics have been increasingly used in biomedical research in recent years because of their good aqueous dispersibility and biocompatibility, which is essential for nanoparticles to enter cells. SPIONs coated with polyethylene glycol (PEG) and polyethyleneimine (PEI) (PEG/PEI-SPIONs) were synthesized by high temperature thermal decomposition method, then PEG/PEI-SPIONs were modified with 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) by hydrogen bonding interactions to obtain DMPC-SPIONs, and thermogravimetric analysis showed that the mass fraction of DMPC on the surface of SPIONs was 31.7wt%. The research on the uptake of PEG/PEI-SPIONs and DMPC-SPIONs by PC-12 cells showed that much more DMPC-SPIONs entered the cells, whereas relatively fewer PEG/PEI-SPIONs were observed in the cells. The results showed that DMPC played an important role in increasing the entry of SPIONs into cells. DMPC-SPIONs were densely distributed in the lysosomes, mitochondria, endoplasmic reticulum and around the nuclei in PC-12 cells. Some DMPC-SPIONs remained in the vicinity of cilia. The endocytosis process of DMPC-SPIONs was observed. DMPC-SPIONs have great potential to be used as hyperthermia agents, MRI contrast agents and drug transportation carriers.
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