Regenerated Silk Fibroin/Calcium Alginate Sponge for Tissue Engineering Applications: Preparation, Characterization and in Vivo and in Vitro Properties
SHI Min, TAO Sijie, LI Dan, WANG Xin, XU Shui, ZHU Yong
State key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
Abstract: Regenerated silk fibroin/calcium alginate composite materials were prepared by freeze drying using regenerated silk fibroin and sodium alginate as main raw materials. SEM was used to observed the internal structure of silk fibroin/calcium material. In addition the capability of water absorption, water retention was studied and the mechanical properties was tested: all of the eight materials have interconnected three-dimensional porous structures uniformly distributed with apertures between 50 μm and 300 μm.Their water absorption rate is between 589.09% and 1 908.31%. The water retention rate range from 227.34% to 1 542.93%. All materials have good reusability and fast water absorption rate. The tensile strength under wet condition is between 0.066 9 MPa and 0.237 5 MPa.Through the subcutaneous implant test of SD rats we found that all of the materials are biocompatible and degradable.
石敏, 陶思洁, 李丹, 王鑫, 徐水, 朱勇. 面向组织工程应用的再生丝素/海藻酸钙海绵:制备、表征及体内、体外性能研究[J]. 材料导报, 2020, 34(4): 4158-4165.
SHI Min, TAO Sijie, LI Dan, WANG Xin, XU Shui, ZHU Yong. Regenerated Silk Fibroin/Calcium Alginate Sponge for Tissue Engineering Applications: Preparation, Characterization and in Vivo and in Vitro Properties. Materials Reports, 2020, 34(4): 4158-4165.
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2020, Vol. 34 
