Abstract: Microbially-induced calcium carbonate precipitation has been widely used for the surface treatment of all sorts of porous media, for the purpose of improving the durability and extending the service life. By using ureolytic type microorganism, two methods including liquid immersion and agar immobilization were employed for the surface treatment of concrete. After treatment for one week, the capillary water absorption coefficient, water permeation coefficient, and rapid chloride permeation (RCP) of concrete were tested. The contact angle of the surfaces and the thickness of the precipitates were measured. The morphology and composition of the precipitates were comprehensively studied by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD) analyses. Results revealed that the thickness of the microbial precipitated coating layer was about 10 μm. Although the layer precipitated by agar immobilization showed hydrophilicity and was thinner than that by liquid immersion, much denser layer of calcium carbonate was obtained by immobilization method since agar provided favorable microenvironment for the growth of bacteria. Thus, the resistance to permeability of concrete can be effectively enhanced, in particular, the water permeation coefficient was decreased more than 80% by immobilization technique. Meanwhile, agar immobilization has significant values in practical application.
徐晶, 王先志. 浸渍及固载法用于混凝土微生物表面处理对比研究[J]. 材料导报, 2018, 32(24): 4276-4280.
XU Jing, WANG Xianzhi. A Comparison Investigation on the Immersion and Immobilization Methods Applied in the Microbial Surface Treatment for Concrete. Materials Reports, 2018, 32(24): 4276-4280.
1 Boquet E, Boronat A, Ramos-Cormenzana A. Production of calcite (calcium carbonate) crystals by soil bacteria is a common phenomenon[J].Nature,1973,246:527. 2 Gollapudi U K, Knutson C L, Bang S S, et al. A new method for controlling leaching through permeable channels[J].Chemosphere,1995,30(4):695. 3 Xu J. Research progress of concrete crack remediation based on microbially mineral precipitation[J].Journal of Zhejiang University (Engineering Science),2012,46(11):2020(in Chinese). 徐晶.基于微生物矿化沉积的混凝土裂缝修复研究进展[J].浙江大学学报(工学版),2012,46(11):2020. 4 Achal V, Mukherjee A. A review of microbial precipitation for sustainable construction[J].Construction and Building Materials,2015,93:1224. 5 De Muynck W, De Belie N, Verstraete W. Microbial carbonate precipitation in construction materials: A review[J].Ecological Engineering,2010,36(2):118. 6 Wang J Y, Ersan Y C, Boon N, et al. Application of microorganisms in concrete: A promising sustainable strategy to improve concrete durability[J].Applied Microbiology and Biotechnology,2016,100(7):2993. 7 Qian C, Wang J, Wang R, et al. Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii[J].Materials Science and Engineering:C,2009,29(4):1273. 8 De Muynck W, Cox K, De Belie N, et al. Bacterial carbonate preci-pitation as an alternative surface treatment for concrete[J].Construction and Building Materials,2008,22(10):875. 9 De Muynck W, Debrouwer D, De Belie N, et al. Bacterial carbonate precipitation improves the durability of cementitious materials[J].Cement and Concrete Research,2008,38(7):1005. 10 Nosouhian F, Mostofinejad D, Hasheminejad H. Influence of biodeposition treatment on concrete durability in a sulphate environment[J].Biosystems Engineering,2015,133:141. 11 Xu J, Yao W, Jiang Z W. Non-ureolytic bacterial carbonate precipitation as a surface treatment strategy on cementitious materials[J].Journal of Materials in Civil Engineering,2014,26(5):983. 12 Wang R X, Qian C X, Wang J Y. Bio-deposition of a calcite layer on cement-based materials by brushing with agar-immobilised bacteria[J].Advances in Cement Research,2011,23(4):185. 13 Wang J, Qian C, Wang R, et al.Surface protection of cement based materials by a CaCO3 layer produced by alginate-immobilized bacteria[J].Jounal of Functional Materials,2009,40(2):348(in Chinese). 王剑云,钱春香,王瑞兴,等.海藻酸钠固载菌株在水泥基材料表面防护中的应用研究[J].功能材料,2009,40(2):348. 14 Wang R, Qian C. Restoration of defects on cement-based materials suface by bacteria immobilized in agar[J].Journal of Building Mate-rials,2013,16(6):942(in Chinese). 王瑞兴,钱春香.琼脂固载微生物矿化修复水泥基材料表面缺陷[J].建筑材料学报,2013,16(6):942. 15 Wang R, Qian C, Wang J, et al. Different targeted methods of microbiologically deposited CaCO3 layer on hardened cement paste surface[J].Journal of the Chinese Ceramic Society,2008,36(10):1378(in Chinese). 王瑞兴,钱春香,王剑云,等.水泥石表面微生物沉积碳酸钙覆膜的不同工艺[J].硅酸盐学报,2008,36(10):1378. 16 Zhu F, Li G, Du H, et al. A new surface-modified technology of cement mortar using calcium carbonate biodeposition[J].Jounal of Functional Materials,2013,44(5):700(in Chinese). 朱飞龙,李庚英,杜虹,等.一种基于微生物沉积的水泥砂浆表面改性技术[J].功能材料,2013,44(5):700.