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《材料导报》期刊社  2018, Vol. 32 Issue (12): 1967-1972    https://doi.org/10.11896/j.issn.1005-023X.2018.12.005
  材料研究 |
明胶/掺锶β-磷酸三钙/硫酸钙复合多孔支架的制备与性能
秦晓素,黄洁,雷云,杨泽斌,陈庆华,颜廷亭
昆明理工大学材料科学与工程学院,昆明 650500
Fabrication and Properties of Porous Gelatin/Strontium-doped β-tricalcium Phosphate/Calcium Sulfate Scaffolds
QIN Xiaosu, HUANG Jie, LEI Yun,YANG Zebin, CHEN Qinghua, YAN Tingting
Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming 650500
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摘要 以掺锶β-磷酸三钙/硫酸钙为原料,利用搅拌喷雾干燥法制备出掺锶β-磷酸三钙/硫酸钙复合小球,再将明胶与制备的复合小球复合,制备出明胶/掺锶β-磷酸三钙/硫酸钙复合生物支架。通过X射线衍射仪、扫描电镜、红外光谱仪等分析了复合多孔支架的成分、形貌以及结构特征,并研究了复合生物支架的降解性、孔隙率、力学性能和细胞毒性等。结果表明:该复合多孔生物支架具有不规则的孔洞结构,孔径在0.5~1 mm之间,且平均孔隙率达到(63±1.77)%,基本能满足骨组织工程支架对孔隙率的要求;复合多孔支架具有良好的抗压强度,其平均抗压强度在(6.3±0.05) MPa左右;该复合多孔支架无细胞毒性,具有较好的降解速率,因此该支架在骨组织修复方面具有良好的应用前景。    
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秦晓素
黄洁
雷云
杨泽斌
陈庆华
颜廷亭
关键词:  明胶  掺锶β-磷酸三钙  硫酸钙  复合小球  多孔支架    
Abstract: The present work fabricated the calcium sulfate/strontium-doped β-tricalcium phosphate composite spherical pellets through the stirring spray drying method and using the calcium sulfate/strontium-doped β-TCP as raw material. Subsequently the composite spherical pellets were combined with gelatin, to get porous gelatin/calcium sulfate/strontium-doped β-tricalcium phosphate scaffolds. We employed X-ray diffraction (XRD),scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR), etc. to examine the products’ chemical composition, morphology and microstructure, and also investigated the degradability, porosity, mechanical properties and cytotoxicity. The results reveal that the composite porous scaffolds have an irregular pore structure with pore size ranging from 0.5 mm to 1.0 mm and the porosity of about (63±1.77)%, which could meet the requirements of scaffold for bone tissue engineering. Besides, the favorable compressive strength (average value (6.3±0.05) MPa) of the resul-tant scaffolds were observed. The cytotoxicity test showed that, the composite scaffolds exhibit no cytotoxic effect and good degradation rate and thereby can find potential application in bone tissue repair.
Key words:  gelatin    strontium-doped β-tricalcium phosphate    calcium sulfate    composite spheres    porous scaffold
               出版日期:  2018-06-25      发布日期:  2018-07-20
ZTFLH:  TB321  
  O649.4  
基金资助: 国家自然科学基金(31260228);云南省教育厅基金(2014Y074)
作者简介:  秦晓素:女,1991年生,硕士,研究方向为生物骨科材料 E-mail:769860685@qq.com 颜廷亭:通信作者,男,1983年生,博士,副教授,硕士研究生导师,主要研究方向为生物医用材料 E-mail:itty@foxmail.com
引用本文:    
秦晓素,黄洁,雷云,杨泽斌,陈庆华,颜廷亭. 明胶/掺锶β-磷酸三钙/硫酸钙复合多孔支架的制备与性能[J]. 《材料导报》期刊社, 2018, 32(12): 1967-1972.
QIN Xiaosu, HUANG Jie, LEI Yun,YANG Zebin, CHEN Qinghua, YAN Tingting. Fabrication and Properties of Porous Gelatin/Strontium-doped β-tricalcium Phosphate/Calcium Sulfate Scaffolds. Materials Reports, 2018, 32(12): 1967-1972.
链接本文:  
http://www.mater-rep.com/CN/10.11896/j.issn.1005-023X.2018.12.005  或          http://www.mater-rep.com/CN/Y2018/V32/I12/1967
1 Schwartz C E, Martha J F, Kowalski P, et al. Prospective evaluation of chronic pain associated with posterior autologous iliac crest bone graft harvest and its effect on postoperative outcome[J]. Health and Quality of Life Outcomes,2009,7(1):49.
2 Henkel J, Woodruff M A, Epari D R, et al. Bone regeneration based on tissue engineering conceptions—A 21st century perspective[J]. Bone Research,2013,1(3):216.
3 Burg K J L, Porter S, Kellam J F. Biomaterial developments for bone tissue engineering[J]. Biomaterials,2000,21(23):2347.
4 Zhang Zhida, Jiang Xiaobing, Shen Gengyang, et al. Research advancement of calcium phosphate and calcium sulfate scaffolds in bone tissue engineering[J]. Chinese Journal of Tissue Engineering Research,2016,20(8):1203(in Chinese).
张志达,江晓兵,沈耿杨,等.磷酸钙及硫酸钙支架在骨组织工程中的研究进展[J].中国组织工程研究,2016,20(8):1203.
5 Lichte P, Pape H C, Pufe T, et al. Scaffolds for bone healing: Concepts, materials and evidence[J]. Injury,2011,42(6):569.
6 Chang B S, Hong K S, Youn H J, et al. Osteoconduction at porous hydroxyapatite with various pore configurations[J]. Biomaterials,2000,21(12):1291.
7 Olszta M J, Cheng X, Jee S S, et al. Bone structure and formation: A new perspective[J]. Materials Science and Engineering R: Reports,2007,58(3):77.
8 Ma Xinfang, Zhang Jingying. Development of bone tissue enginee-ring scaffold materials[J]. Chinese Journal of Tissue Engineering Research,2014,18(30):4895(in Chinese).
马新芳,张静莹.骨组织工程支架材料的研究现状与应用前景[J].中国组织工程研究,2014,18(30):4895.
9 Sariibrahimoglu K, Wolke J G C, Leeuwenburgh S C G, et al. Characterization of α/β-TCP based injectable calcium phosphate cement as a potential bone substitute[J].Key Engineering Materials,2013,529:157.
10 Sugawara A, Asaoka K, Ding S J. Calcium phosphate-based cements: Clinical needs and recent progress[J]. Journal of Materials Chemistry B,2013,1(8):1081.
11 poner P, Urban K, Kucera T, et al. The use of interconnected β-tricalcium phosphate as bone substitute after curettege of benign bone tumours[J]. European Journal of Orthopaedic Surgery & Traumatology,2011,21(4):235.
12 Shen Zhichao, Wu Jiahui, Tian Zhongqing, et al. Effect of CaO on the structure and properties of titanium barium silicate glass[J]. Journal of Chongqing University of Technology(Natural Science),2017(11):88(in Chinese).
沈志超,吴家辉,田中青,等.CaO对钛钡硅酸玻璃结构和性能的影响[J].重庆理工大学学报(自然科学),2017(11):88.
13 Cacciotti I, Bianco A. High thermally stable Mg-substituted trical-cium phosphate via precipitation[J]. Ceramics International,2011,37(1):127.
14 Hoppe A, Güldal N S, Boccaccini A R. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics[J]. Biomaterials,2011,32(11):2757.
15 Lehmann G, Cacciotti I, Palmero P, et al. Differentiation of osteoblast and osteoclast precursors on pure and silicon-substituted synthesized hydroxyapatites[J]. Biomedical Materials,2012,7(5):055001.
16 Lehmann G, Palmero P, Cacciotti I, et al. Design, production and biocompatibility of nanostructured porous HAp and Si-HAp ceramics as three-dimensional scaffolds for stem cell culture and differentiation[J]. Ceramics-Silikaty,2010,54(2):90.
17 Zhai Yujia. Preparation and characteristics of Sr-doped bioglass/biphasic tricalcium phosphate cements[D].Tianjin: Tianjin University,2012(in Chinese).
翟羽佳.含锶生物玻璃/双相磷酸三钙骨水泥的制备及性能研究[D].天津:天津大学,2012.
18 Leventis M D, Fairbairn P, Dontas I, et al. Biological response to β-tricalcium phosphate/calcium sulfate synthetic graft material: An experimental study[J]. Implant Dentistry,2014,23(1):37.
19 Beuerlein M J S, McKee M D. Calcium sulfates: What is the evidence?[J]. Journal of Orthopaedic Trauma,2010,24:S46.
20 Thomas M V, Puleo D A. Calcium sulfate: Properties and clinical applications[J]. Journal of Biomedical Materials Research Part B: Applied Biomaterials,2009,88(2):597.
21 Khan Y, Yaszemski M J, Mikos A G, et al. Tissue engineering of bone: Material and matrix considerations[J]. The Journal of Bone & Joint Surgery,2008,90(Supplement 1):36.
22 Bonilla J, Bittante A M Q B, Sobral P J A. Thermal analysis of gelatin-chitosan edible film mixed with plant ethanolic extracts[J]. Journal of Thermal Analysis & Calorimetry,2017,130(2):1221.
23 Gomes S R, Rodrigues G, Martins G G, et al. In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: A comparative study[J]. Materials Science and Engineering: C,2015,46:348.
24 Liang Haojun, Li Ruiyan, Liu Guancong, et al. Design of the po-rous orthopedic implants: Research and application status[J]. Chinese Journal of Tissue Engineering Research,2017,21(15):2410(in Chinese).
梁豪君,李瑞延,刘贯聪,等.医用骨科假体植入物多孔结构设计的研究和临床应用现状[J].中国组织工程研究,2017,21(15):2410.
25 Tsuruga E, Takita H, Itoh H, et al. Pore size of porous hydroxya-patite as the cell-substratum controls BMP-induced osteogenesis1[J]. Journal of biochemistry,1997,121(2):317.
26 Koc N, Timucin M, Korkusuz F. Fabrication and characterization of porous tricalcium phosphate ceramics[J]. Ceramics International,2004,30(2):205.
27 Du B, Liu W, Deng Y, et al. Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo[J]. International Journal of Nanomedicine,2015,10:2555.
28 Aggarwal H, Kumar P, Singh R D, et al. Ocular rehabilitation following socket reconstruction with amniotic membrane transplantation with failed primary hydroxyapatite implant post enucleation[J]. Contact Lens and Anterior Eye,2015,38(1):64.
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