利用Triton X-100提升巴氏芽孢杆菌脲酶活性的效果

doi:10.11896/cldb.23060069

材料导报

2024, Vol. 38

Issue (1): 23060069-7 https://doi.org/10.11896/cldb.23060069

高分子与聚合物基复合材料

利用Triton X-100提升巴氏芽孢杆菌脲酶活性的效果

肖瑶, 邓华锋^*, 李建林, 熊雨, 程雷

三峡库区地质灾害教育部重点实验室(三峡大学),湖北宜昌 443002

Effect of Triton X-100 on Improving Urease Activity of Sporosarcina Pasteurii

XIAO Yao, DENG Huafeng^*, LI Jianlin, XIONG Yu, CHENG Lei

Key Laboratory of Geological Hazards on Three Gorges Reservoir Area of Ministry of Education, China Three Gorges University, Yichang 443002, Hubei, China

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摘要微生物诱导碳酸盐沉积(MICP)技术是近年来发展起来的一种新型绿色加固技术,如何提升MICP技术的加固效能一直是学者关注的热点,本工作重点结合巴氏芽孢杆菌产脲酶原理和非离子表面活性剂的特点,设计采用非离子表面活性剂Triton X-100提升MICP效能的试验研究。结果表明:(1)在单因素试验结果的基础上,通过响应面试验分析确定了活性剂的最优处理条件,即掺量1.37%、处理时间5.02 h、处理温度34.28 ℃、菌胶比1∶2.11;(2)采用最优条件处理的巴氏芽孢杆菌对钙质砂进行加固后,钙质砂固化体碳酸钙含量和无侧限抗压强度分别提升了25.48%和22.83%;(3)非离子表面活性剂Triton X-100增大了巴氏芽孢杆菌的细胞膜通透性,一方面使得更多的胞内脲酶释放到细胞外,同时更多的尿素分子进入到细胞内,促进了尿素水解,提升了碳酸钙的生成速率;另一方面使得以细菌为核点生成的碳酸钙胶凝体的结构更为致密,在这两方面作用下,MICP效能得到有效提升。相关思路和研究结果可为MICP加固效果的提升提供新的参考和思路。

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关键词: 微生物诱导碳酸盐沉积脲酶非离子表面活性剂细胞膜通透性响应面法

Abstract: Microbially induced carbonate precipitation (MICP) technology is a new green reinforcement technology developed in recent years. How to improve the reinforcement efficiency of MICP has always been a focus of scholars’ attention. This work focuses on the principle of urease produced by Sporosarcina pasteurii and the characteristics of non-ionic surfactant. The experiment of improving MICP efficiency with non-ionic surfactant Triton X-100 was designed. The results showed that:(Ⅰ) based on the results of single factor experiment, response surface experiment was designed to analyze and determine the optimal treatment conditions of the surfactant, namely, the dosage was 1.37%, the treatment time was 5.02 h, the treatment temperature was 34.28 ℃, and the ratio of bacteria to cementation solution was 1∶2.11;(Ⅱ) the calcium carbonate content and unconfined compressive strength of the solidified body increased by 25.48% and 22.83% respectively after the calcareous sand was reinforced by Sporosarcina pasteurii under the optimal conditions;(Ⅲ) the non-ionic surfactant Triton X-100 increases the permeability of the cell membrane of Sporosarcina pasteurii. On the one hand, more intracellular urease is released to the outside of the cell, while more urea enters the cell, which promotes the hydrolysis of urea and the formation rate of carbonate. On the other hand, the calcium carbonate gel structure generates with bacteria as the core more compact. Under the effect of these two aspects, the MICP efficiency is effectively improved. Relevant ideas and research results can provide new reference and ideas for the improvement of MICP reinforcement effect.

Key words: microbially induced carbonate precipitation (MICP) urease non-ionic surfactant cell membrane permeability response surface method

发布日期: 2024-01-16

ZTFLH:

TU521

基金资助: 国家自然科学基金(U22A20600;U2034203)

通讯作者: 邓华锋,三峡大学土木与建筑学院教授、博士研究生导师。2002年武汉水利电力大学(宜昌)建筑工程专业本科毕业,2005年三峡大学土木水电专业硕士毕业后到三峡大学工作至今,2010年武汉大学水利电力专业博士毕业。目前主要从事水电工程边坡变形与稳定分析、库岸边坡水岩相互作用机理及卸荷岩体力学试验与理论等方面的研究工作。授权专利150余项,发表论文200余篇,包括《岩土力学》《岩石力学与工程学报》、Bulletin of Engineering Geology and the Environment、Scientific Reports等。dhf8010@ctgu.edu.cn

作者简介: 肖瑶,2015年6月、2018年6月于三峡大学分别获得工学学士学位和硕士学位。现为三峡大学土木与建筑学院博士研究生,在邓华锋教授、李建林教授的指导下进行研究。目前主要研究领域为环境岩土工程。

引用本文:

肖瑶, 邓华锋, 李建林, 熊雨, 程雷. 利用Triton X-100提升巴氏芽孢杆菌脲酶活性的效果[J]. 材料导报, 2024, 38(1): 23060069-7.
XIAO Yao, DENG Huafeng, LI Jianlin, XIONG Yu, CHENG Lei. Effect of Triton X-100 on Improving Urease Activity of Sporosarcina Pasteurii. Materials Reports, 2024, 38(1): 23060069-7.

链接本文:

http://www.mater-rep.com/CN/10.11896/cldb.23060069 或 http://www.mater-rep.com/CN/Y2024/V38/I1/23060069

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