A Novel Fluorescent Chemosensor for α-Amino Acids and Its Application for the Detection of Protease
CHENG Xiaohong1, QU Shaohua2, ZHONG Zhicheng1
1 Hubei Key Laboratory of Low Dimensional Optoelectronic Materials and Devices,Hubei University of Arts and Science,Xiang-yang 441053; 2 School of Physics and Electronic Engineering, Hubei University of Arts and Science, Xiangyang 441053
Abstract: Complex C1-Cu(Ⅱ) could act as a new displacement-based fluorescent probe for α-amino acids detection. Upon the addition of α-amino acids, it displayed marked changes from non-fluorescence to strongly green fluorescence which could be observed by naked-eyes under a normal UV lamp. Especially, histidine exhibited the highest sensitivity: the fluorescent intensity increased to ~135-fold of the original one with the addition of excess histidine. Moreover, the intensity change at 490 nm was almost linear with the concentrations of histidine. Furthermore, as the hydrolysis of bovine serum albumin (BSA) with the aid of trypsin produces α-amino acids, the complex of C1-Cu(Ⅱ) with BSA could serve as a label-free, selective, fluorescent sensor toward trypsin with “turn-on” fluorescent methods.
程晓红, 屈少华, 钟志成. 一种新型的α-氨基酸荧光传感器及其在酶活性检测中的应用[J]. 《材料导报》期刊社, 2018, 32(14): 2486-2490.
CHENG Xiaohong, QU Shaohua, ZHONG Zhicheng. A Novel Fluorescent Chemosensor for α-Amino Acids and Its Application for the Detection of Protease. Materials Reports, 2018, 32(14): 2486-2490.
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2018, Vol. 32 
