Preparation and Characterization of Porous Collagen-β-tricalcium Phosphate-chondroitin Sulfate Composite Membrane
XUE Yuan1,2, DAN Nianhua1,2, DAN Weihua1,2
1 National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065; 2 Biomedical Engineering Technology Research Center, Sichuan University, Chengdu 610065;
Abstract: The porous collagen-β-tricalcium phosphate-chondroitin sulfate composite membrane was prepared by EDC modification and twice freeze-dried method. The effect of variation of material proportion, pH value and method in materials preparation process on the structure and properties of composite materials were investigated by scanning electron microscope (SEM), X-ray diffraction analyzer (XRD) and atomic force microscope (AFM), FT-IR, porosity measurer, MTT cytotoxicity tester. The results indicated that when the pH value of collagen/HCl solution was 2, the mass ratio of collagen and β-tricalcium phosphate was 1∶2 (m(Col)∶m(β-TCP)=1∶2), the β-TCP′s crystalline phase maintained, the composition was uniform and compact. After EDC modification, the SEM and AFM results showed that the collagen bundles after crosslinking got bigger and thicker obviously, arranged closely in a certain direction. XRD spectrum showed that the characteristic diffraction peaks of β-TCP in composite materials were obvious. The pore size of composite material was about 80-90 μm, the porosity of ternary membrane was (90.76±1.28)%, larger than that of collagen membrane (85.88±0.91)%. Infrared spectra confirmed that Ca2+ of β-TCP and carboxyl of Col were chemically bonded. AFM showed that β-TCP particles had a direct connection with collagen. The MTT cytotoxicity levels of compo-site materials were 1, which was a kind of potential dental restorative material.
1 Carbonell J M, et al. High-density polytetrafluoroethylene membranes in guided bone and tissue regeneration procedures: A literature review [J]. Ont J Oral Maxillofacial Surg,2014,43(1):75. 2 OH T J, Meraw S J, Lee E J, et al. Comparative analysis of collagen membranes for the treatment of implant dehiscence defects [J]. Clin Oral Implants Res,2003,14(1):80. 3 Yu Y, Deng F. The application and progress of barrier membrane in guided bone regeneration [J]. Chin J Oral Implantology,2006,11(3):137(in Chinese). 余粤海,邓飞龙.GBR技术中屏障膜的应用和研究进展[J]. 中国口腔种植学杂志,2006,11(3):137. 4 Jovanovic S A, Hunt D R, Bernard G W, et al. Bone reconstruction following implantation of rhBMP-2 and guided bone regeneration in canine alveolar ridge defects [J]. Clinic Oral Implants Res,2007,18(2):224. 5 Veríssimo D M, Leitao R F, Ribeiro R A, et al. Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation[J]. Acta Biomater,2010,6(10):4011. 6 Tal H, Kozlovsky A, Artzi Z, et al. Long-term bio-degradation of cross-linked and non-cross-linked collagen barriers in human guided bone regeneration[J]. Clin Oral Implants Res,2008,19(3):295. 7 Han X, Fu Y, Zhang F. Advances in research and application of β-tricalcium phosphate/collagen composite in bone tissue engineering [J]. Shanghai J Stomatology,2007,16(1):93(in Chinese). 韩祥永,傅远飞,张富强.β-磷酸三钙、胶原及其复合物在骨组织工程中的应用研究进展[J]. 上海口腔医学,2007,16(1):93. 8 Wang K, Zeng R, Ye Y, et al. Preparation of collagen-chitosan-chondroitin sulfate composite membranes and its characterization [J]. China Leather,2009,38(1):22 (in Chinese). 王康健,曾睿,叶易春,等.胶原-壳聚糖-硫酸软骨素三元膜材料的制备与结构表征[J].中国皮革,2009,38(1):22. 9 Guan B. Preparation and biological evaluation of bone-like collagen fibril based porous tricalcium phosphate/collagen composites [D]. Hangzhou:Zhejiang University,2008(in Chinese). 官丙刚.类骨结构胶原纤维基多孔磷酸三钙/胶原复合材料的制备及其生物学评价[D]. 杭州:浙江大学,2008. 10 Zou C, Weng W, Deng X, et al. Preparation and characterization of porous β-tricalcium phosphate/collagen composites with an integra-ted structure [J]. Biomaterials,2005,26(26):5276. 11 Plepis A, Goissis G, Das-Gupta D K. Dielectric and pyroelectric characterization of anionic and native collagen [J]. Polym Eng Sci,1996,36:2932. 12 Ge J, Wang Y, Zheng Y. Degradable performance and bio-mineralization function of β-tricalcium phosphate/poly (lactic acid) tissue engineering scaffold in SBF [J]. Polym Mater Sci Eng,2011,27(9):119(in Chinese). 葛建华,王迎军,郑裕东.β-磷酸三钙/聚乳酸组织工程支架在模拟体液中的降解和矿化性能[J]. 高分子材料科学与工程,2011,27(9):119. 13 Li G, Zhang Z, Fu Q, et al. Classes of the forms of collagens and their physiological functions [J]. J Shanxi University of Science Technology, 2004,22(3):80(in Chinese). 李国英,张忠楷,付强,等.胶原的形态分类及其生理机能[J].陕西科技大学学报,2004,22(3):80. 14 詹怀宇,李自强,蔡再生.纤维化学与物理[M].北京:化学出版社,2005. 15 Zhong J, Ouyang X, Mei F, et al. Construction of 3D complex of porous β-tricalcium phosphate/collagen scaffold dog periodontal ligament cells [J]. J Peking University:Health Sciences,2007,39(5):507(in Chinese). 钟金晟,欧阳翔英,梅芳,等.多孔β-磷酸三钙/胶原支架与犬牙周膜细胞三维复合体的构建[J]. 北京大学学报:医学版,2007,39(5):507.
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2017, Vol. 31 
