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材料导报  2026, Vol. 40 Issue (6): 25020104-7    https://doi.org/10.11896/cldb.25020104
  无机非金属及其复合材料 |
阳离子碳点抗菌剂的制备与性能
李倩倩1, 刘丽媛2, 张文莉2, 刘勇3, 陈琳2,*, 张利3,*
1 山西医科大学第二医院,太原 030001;
2 太原理工大学新材料界面科学与工程教育部重点实验室,太原 030024;
3 山西白求恩医院(山西医学科学院),太原 030032
Preparation and Properties of Cationic Carbon Dot Antimicrobial Agents
LI Qianqian1, LIU Liyuan2, ZHANG Wenli2, LIU Yong3, CHEN Lin2,*, ZHANG Li3,*
1 The Second Hospital of Shanxi Medical University, Taiyuan 030001, China;
2 MOE Key Laboratory of Interface Science and Engineering in Advanced Materials, Taiyuan University of Technology, Taiyuan 030024, China;
3 Shanxi Bethune Hospital (Shanxi Academy of Medical Sciences), Taiyuan 030032, China
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摘要 阳离子碳点(Cationic carbon dots,CCD)因表面富含正电荷,更容易与带负电荷的细胞壁(膜)发生静电相互作用,破坏细菌结构,导致细菌死亡。本工作以端基为氨基的阳离子化合物乙二胺(EDA)和葡萄糖为原料,通过水热法一步制备CCD抗菌剂。以表面电荷为依据,对EDA浓度、葡萄糖浓度、反应时长、反应温度这四个参数进行优化,对CCD进行结构表征,选用大肠杆菌和金黄色葡萄球菌评价了CCD的抗菌性能。结果表明,在60 mL的EDA浓度为0.5 mol/L、葡萄糖浓度为0.3 mol/L的水溶液中200 ℃下反应4 h,可合成表面电位较高(+38.20 mV)的CCD。该CCD的粒径为(8.07±1.12) nm,对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度均为512 μg/mL,最小杀菌浓度均为1 024 μg/mL,抗菌性能较好;且当其浓度小于2 048 μg/mL时,细胞存活率均高于92%,细胞毒性较低。
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李倩倩
刘丽媛
张文莉
刘勇
陈琳
张利
关键词:  细菌感染  阳离子碳点  一步水热法  抗菌  生物相容材料    
Abstract: Cationic carbon dots(CCDs)show good antimicrobial effects because they can easily interact with negatively charged cell membranes by electrostatic interaction owing to their positively charged surface, destroying bacterial structures and leading to bacterial death. In this work, CCDs were prepared by using one-step hydrothermal method, and using ethylenediamine(EDA, rich in amino terminal group) and glucose as raw materials. The structure and surface charge of CCDs were optimized by regulating the concentration of EDA and glucose, the reaction time, and the reaction temperature. Escherichia coli(E.coli) and Staphylococcus aureus(S.aureus) were selected to characterize the antimicrobial properties. Results showed that CCDs with relatively high surface potential(+38.20 mV) could be synthesized by adopting the optimum reaction condition(0.5 mol/L EDA concentration, 0.3 mol/L of glucose concentration, and reacting in 60 mL water at 200 ℃ for 4 h). The CCDs had particle sizes of (8.07±1.12) nm, minimum inhibitory concentrations for E.coli and S.aureus of both 512 μg/mL, as well as minimum bactericidal concentrations for E.coli and S.aureus of both 1 024 μg/mL, showing satisfactory antimicrobial performance. When the concentration of CCDs was less than 2 048 μg/mL, the cell survival rate was greater than 92%, demonstrating their low cytotoxicity.
Key words:  bacterial infection    cationic carbon dots(CCDs)    one-step hydrothermal method    antibacterium    biocompatible material
出版日期:  2026-03-25      发布日期:  2026-04-03
ZTFLH:  TB332  
基金资助: 山西省基础研究计划(202403021221252;202203021211065);山西省肿瘤医院国家肿瘤区域医疗中心科教培育基金(TD2023003);山西省纳米药物可控缓释技术创新中心(202104010911026)
通讯作者:  *陈琳,博士,太原理工大学新材料界面科学与工程教育部重点实验室副教授、硕士研究生导师,研究方向为纳米碳基生物材料。chenlin01@tyut.edu.cn
张利,博士,山西白求恩医院(山西医学科学院)主任医师,硕士研究生导师,主要研究领域为应用于导尿管的纳米抗菌材料。13935127536@126.com   
作者简介:  李倩倩,硕士,山西医科大学第二医院整形外科主治医师,主要研究领域为纳米抗菌材料在创面愈合中的应用。
引用本文:    
李倩倩, 刘丽媛, 张文莉, 刘勇, 陈琳, 张利. 阳离子碳点抗菌剂的制备与性能[J]. 材料导报, 2026, 40(6): 25020104-7.
LI Qianqian, LIU Liyuan, ZHANG Wenli, LIU Yong, CHEN Lin, ZHANG Li. Preparation and Properties of Cationic Carbon Dot Antimicrobial Agents. Materials Reports, 2026, 40(6): 25020104-7.
链接本文:  
https://www.mater-rep.com/CN/10.11896/cldb.25020104  或          https://www.mater-rep.com/CN/Y2026/V40/I6/25020104
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