多孔CoNi/NC纳米酶的制备及在比色法检测Mn2+中的应用研究

doi:10.11896/cldb.25020005

材料导报

2026, Vol. 40

Issue (3): 25020005-8 https://doi.org/10.11896/cldb.25020005

金属与金属基复合材料

多孔CoNi/NC纳米酶的制备及在比色法检测Mn²⁺中的应用研究

公海龙, 沈思宇, 侯晓峰, 马勤勤, 王学东^*, 刘婷婷^*

苏州科技大学环境科学与工程学院,江苏省环境科学与工程重点实验室,江苏苏州 215009

Preparation of Porous CoNi/NC Nanozyme and Its Application in Colorimetric Detection of Mn²⁺

GONG Hailong, SHEN Siyu, HOU Xiaofeng, MA Qinqin, WANG Xuedong^*, LIU Tingting^*

Jiangsu Key Laboratory of Environmental Science and Engineering, School of Environmental Science and Engineering, Suzhou University of Science and Techno-logy, Suzhou 215009, Jiangsu, China

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摘要通过高温煅烧法制备了具有类氧化物酶活性的多孔CoNi双金属氮掺杂碳纳米酶(CoNi/NC)。该纳米酶可以在无H₂O₂条件下直接催化显色底物3,3′,5,5′-四甲基联苯胺(TMB)变蓝。得益于该纳米酶双金属组分与多孔结构的协同作用,其类氧化物酶催化活性优异。由动力学实验可得,其K_m为(0.919±0.283) mmol/L,V_max为(3.850±0.707)×10^-8 mol·L^-1·s^-1,对底物表现出较强的亲和力和较大的反应速率。由自由基清除实验可得,在TMB被氧化变色过程中,·OH和·O₂^-起关键作用。利用Mn²⁺可以与显色产物络合使溶液体系褪色的现象,构建了比色检测Mn²⁺含量的新方法。该方法线性范围为0.005～0.04 mmol/L,检出限低至0.001 7 mmol/L,且在实际水样中得到成功应用。为进一步提高方法的便捷性,使该纳米酶凝胶化,探究其在实际样品检测中应用的可能性,以期为比色检测方法集成化提供新思路。

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关键词: 多孔CoNi/NC纳米酶类氧化物酶活性比色法 Mn²⁺检测

Abstract: Porous CoNi bimetallic nitrogen-doped carbon nanozyme (CoNi/NC) with oxidase-like activity was prepared by high-temperature calcination. This nanozyme can directly catalyze the chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine(TMB) to produce a blue coloration wit-hout the need for H₂O₂. The excellent peroxidase-like catalytic performance of this nanozyme is attributed to the synergistic effect of its bimetallic components and its porous structure. Kinetic studies revealed that the K_m value is (0.919±0.283) mmol/L, and the V_max is (3.850±0.707)×10^-8 mol·L^-1·s^-1, indicating strong substrate affinity and a high reaction rate. Free radical scavenging experiments demonstrated that ·OH and ·O₂^- radicals play crucial roles in the oxidation and color change process of TMB. Leveraging the phenomenon that Mn²⁺ can complex with the chromogenic product to cause solution decolorization, we developed a novel colorimetric method for detecting Mn²⁺ content. This method exhibits a linear range of 0.005—0.04 mmol/L and a detection limit as low as 0.001 7 mmol/L, and has been successfully applied to real water samples. To enhance the practicality of this method, the nanozyme was immobilized in a gel matrix, exploring its potential for detecting actual samples and providing new insights into the integration of colorimetric detection methods.

Key words: porous CoNi/NC nanozyme oxidase-like activity colorimetric method Mn²⁺ detection

发布日期: 2026-02-13

ZTFLH:	X832
	O657.3

基金资助: 国家自然科学基金(22076134);江苏省重点研发计划(BE2022733)

通讯作者: ^*王学东,博士,苏州科技大学环境科学与工程学院教授、博士研究生导师、研究员。长期从事行业性污染物绿色环保分析技术开发研究。
刘婷婷,硕士,苏州科技大学环境科学与工程学院实验师。目前主要从事纳米酶催化材料、新型检测分析方法和样品前处理方法等方面的研究。

作者简介: 公海龙,苏州科技大学环境科学与工程学院硕士研究生,在王学东教授的指导下进行研究。目前主要研究方向为纳米酶传感器与环境检测。

引用本文:

公海龙, 沈思宇, 侯晓峰, 马勤勤, 王学东, 刘婷婷. 多孔CoNi/NC纳米酶的制备及在比色法检测Mn²⁺中的应用研究[J]. 材料导报, 2026, 40(3): 25020005-8.
GONG Hailong, SHEN Siyu, HOU Xiaofeng, MA Qinqin, WANG Xuedong, LIU Tingting. Preparation of Porous CoNi/NC Nanozyme and Its Application in Colorimetric Detection of Mn²⁺. Materials Reports, 2026, 40(3): 25020005-8.

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https://www.mater-rep.com/CN/10.11896/cldb.25020005 或 https://www.mater-rep.com/CN/Y2026/V40/I3/25020005

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