玻璃载银抗菌材料的Ag<sup>+</sup>溶出性质及与大肠杆菌作用机理

doi:10.11896/j.issn.1005-023X.2018.16.001

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Abstract The antimicrobial activity and antibacterial mechanism of as-prepared Ag-doped glass antimicrobial material against E. coli was investigated. The performance of silver ions release from the Ag-doped glass was also discussed. The sample was amorphous which was tested by X-ray diffractomer. The size D50 of the sample powder was under 10 μm and the range of particle size distribution was wider. The minimal inhibitory concentration (MIC) of the sample against the selected bacteria was 0.2 mg/mL,which mean the material had good antibacterial properties. The effect on bacteriostatic could be neglected when the sample particle size distribution under 15 μm. The dissolubility of Ag⁺was investigated by atomic absorption spectrometry (AAS).It was found that the dissolution amount of Ag⁺ was increased with time, and the smaller the particle size of the powder, the easier it is to dissolve Ag. Ag⁺ solution was significantly lower than that of pure sample after mixing with E. coli. The mixture of Ag-doped glass antimicrobial material and E. coli was solidified and stained, and then observed by transmission electron microscopy (TEM) before and after cutting into slices. The images showed that the treated E. coli cells were damaged and collapsed. TEM images of thin sections showed that the cytoplasm of the E. coli appeared vacuolar degeneration. It was suggested that the Ag⁺ dissolved into the solution, and then attacked nucleus, resulting in death of the cell.

Key words： Ag-doped glass antimicrobial material escherichia coli(E. coli) silver ions release

Published: 18 September 2018

CLC number:

TQ174

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	Articles by authors
	WANG Jing
	WANG Xiaoyan
	SHUI Zhonghe
	JI Zhijiang
	ZHAO Chunyan
	LIU Ruirui

Cite this article:

WANG Jing,WANG Xiaoyan,SHUI Zhonghe, et al. Properties of Silver Ions Release and Antibacterial Mechanism Against E. coli of Ag-doped Glass Antimicrobial Material[J]. Materials Reports, 2018, 32(16): 2709-2714.

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http://www.mater-rep.com/EN/10.11896/j.issn.1005-023X.2018.16.001 OR http://www.mater-rep.com/EN/Y2018/V32/I16/2709

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