一种新型的α-氨基酸荧光传感器及其在酶活性检测中的应用

doi:10.11896/j.issn.1005-023X.2018.14.029

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Abstract Complex C1-Cu(Ⅱ) could act as a new displacement-based fluorescent probe for α-amino acids detection. Upon the addition of α-amino acids, it displayed marked changes from non-fluorescence to strongly green fluorescence which could be observed by naked-eyes under a normal UV lamp. Especially, histidine exhibited the highest sensitivity: the fluorescent intensity increased to ~135-fold of the original one with the addition of excess histidine. Moreover, the intensity change at 490 nm was almost linear with the concentrations of histidine. Furthermore, as the hydrolysis of bovine serum albumin (BSA) with the aid of trypsin produces α-amino acids, the complex of C1-Cu(Ⅱ) with BSA could serve as a label-free, selective, fluorescent sensor toward trypsin with “turn-on” fluorescent methods.

Key words： displacement approach fluorescence enhancement α-amino acids protease solubility

Published: 31 July 2018

CLC number:

O622.4

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	Articles by authors
	CHENG Xiaohong
	QU Shaohua
	ZHONG Zhicheng

Cite this article:

CHENG Xiaohong,QU Shaohua,ZHONG Zhicheng. A Novel Fluorescent Chemosensor for α-Amino Acids and Its Application for the Detection of Protease[J]. Materials Reports, 2018, 32(14): 2486-2490.

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http://www.mater-rep.com/EN/10.11896/j.issn.1005-023X.2018.14.029 OR http://www.mater-rep.com/EN/Y2018/V32/I14/2486

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