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材料导报  2026, Vol. 40 Issue (7): 25050125-6    https://doi.org/10.11896/cldb.25050125
  高分子与聚合物基复合材料 |
基于半纤维素双重交联的pH响应性干酪乳杆菌微球构建及性能研究
刘静茹1, 周雪松1,2,*, 沈书震1
1 华南理工大学轻工科学与工程学院,广州 510641
2 先进造纸与纸基材料全国重点实验室,广州 510641
Construction and Performance of pH-responsive Lactobacillus casei Microspheres Based on Hemicellulose Double Cross-linking
LIU Jingru1, ZHOU Xuesong1,2,*, SHEN Shuzhen1
1 School of Light Industry and Engineering,South China University of Technology, Guangzhou 510641, China
2 State Key Laboratory of Advanced Paper-making and Paper-based Materials, Guangzhou 510641, China
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摘要 作为一种常见的益生菌,干酪乳杆菌(Lactobacillus casei)在改善肠道健康与提高免疫力方面效果显著,但直接服用将引发菌群失活、安全风险和剂量失控等问题。因此,亟需开发一种安全有效的递送材料。本工作选用一类来源广泛的膳食纤维——玉米芯半纤维素——为原料,通过接枝共聚改性,将以聚丙烯酸(PAA)为主的聚合物链段引入其侧链中,以戊二醛-CaCl2为复合交联体系,经喷雾干燥法制得具有pH响应性的生物质基微球。研究结果表明:戊二醛-CaCl2复合交联微球的粒径(122.01 μm)显著大于单一戊二醛交联微球(16.57 μm),干酪乳杆菌的包埋率从86.68%提升至92.70%,菌体活性从98.32%提高至98.45%。在模拟胃肠液中的释放行为显示,复合交联微球在模拟胃液中的存活率均超过60%,在模拟肠液中30 min内益生菌的释放率达80%,表现出良好的pH响应性。FTIR、SEM等表征结果表明,戊二醛-CaCl2复合交联体系与改性半纤维素之间形成离子键和共价键,这种协同交联作用可提高微球的结构稳定性、益生菌的包埋率和菌体存活率。本工作为益生菌的绿色智能递送系统开发提供了一种新策略。
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刘静茹
周雪松
沈书震
关键词:  半纤维素  干酪乳杆菌  喷雾干燥  离子交联    
Abstract: As a common probiotic, Lactobacillus casei has shown remarkable efficacy in improving intestinal health and boosting immunity. However, its direct oral administration may lead to issues such as bacterial inactivation, safety risks, and uncontrolled dosage. Therefore, there is an urgent need to develop a safe and effective delivery material. In this study, a widely available dietary fiber — hemicellulose derived from corncob — was selected as the raw material. Through graft copolymerization modification, polymer segments primarily composed of polyacrylic acid (PAA) were introduced into its side chains. Using a glutaraldehyde-CaCl2 composite crosslinking system and the spray-drying method, pH-responsive biomass-based microspheres were successfully prepared. The results show that the diameter of the glutaraldehyde-CaCl2 composite-crosslinked microspheres (122.01 μm) was significantly larger than that of microspheres crosslinked with glutaraldehyde alone (16.57 μm). The encapsulation efficiency of Lactobacillus casei increased from 86.68% to 92.70%, and the bacterial viability rose from 98.32% to 98.45%. Release beha-vior in simulated gastrointestinal fluids demonstrated that the composite-crosslinked microspheres maintained a survival rate above 60% in simulated gastric fluid, while achieving a release rate of 80% within 30 min in simulated intestinal fluid, demonstrating favorable pH-responsive properties. Characterization results from FTIR and SEM revealed that ionic and covalent bonds were formed between the glutaraldehyde-CaCl2 compo-site crosslinking system and the modified hemicellulose. This synergistic crosslinking interaction enhanced the structural stability of the microspheres, as well as the encapsulation efficiency and survival rate of the probiotics. This study provides a novel strategy for the development of green and intelligent delivery systems for probiotics.
Key words:  hemicellulose    Lactobacillus casei    spray drying    ion crosslinking
发布日期:  2026-04-16
ZTFLH:  Q5503  
基金资助: 广东省基础与应用基础研究基金(2022A1515011448)
通讯作者:  *周雪松,博士,华南理工大学轻工科学与工程学院副研究员、硕士研究生导师,主要从事天然高分子改性及其功能材料的研究。xszhou@scut.edu.cn   
作者简介:  刘静茹,华南理工大学轻工科学与工程学院硕士研究生,在周雪松副研究员的指导下开展多糖基微球材料的制备与研究。
引用本文:    
刘静茹, 周雪松, 沈书震. 基于半纤维素双重交联的pH响应性干酪乳杆菌微球构建及性能研究[J]. 材料导报, 2026, 40(7): 25050125-6.
LIU Jingru, ZHOU Xuesong, SHEN Shuzhen. Construction and Performance of pH-responsive Lactobacillus casei Microspheres Based on Hemicellulose Double Cross-linking. Materials Reports, 2026, 40(7): 25050125-6.
链接本文:  
https://www.mater-rep.com/CN/10.11896/cldb.25050125  或          https://www.mater-rep.com/CN/Y2026/V40/I7/25050125
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