| BIOMEDICAL MATERIALS |
|
|
|
|
|
| Effect of Surface Microroughening of Titanium Alloy on Osteoblast Adhesion and Proliferation Behavior |
| HUANG Run1,WANG Qingping1,ZHANG Lan2,CHEN Xiangyang3,HU Dong4
|
1 School of Materials Science and Engineering, Anhui University of Science and Technology, Huainan 232001;
2 State Key Laboratory for Mechanical Behavior of Materials, Xi’an Jiaotong University, Xi’an 710049;
3 School of Mechanical Engineering, Anhui University of Science and Technology, Huainan 232001;
4 Medical Inspection Center, Anhui University of Science and Technology, Huainan 232001 |
|
|
|
|
Abstract Ti-25Nb-3Mo-3Zr-2Sn (TLM) titanium alloy was treated via surface mechanical attrition treatment (SMAT) for 15 min in this paper, after which the treated and untreated titanium alloy samples were respectively cultured with osteoblast cells for 1 h and 24 h. XRD, OM, TEM, SEM, EDX and CLSM were adopted to analyze the structure of the samples, and the obtained results revealed that SMAT process did not alter the phase composition, grain size and chemical composition of TLM alloy. Both the treated and untreated titanium alloys were composed of the same solely β phase, in the same grain size of about 10—20 μm and without newly-brought impurity elements. However, this treatment would alter the surface roughness of TLM alloy. The untreated sample possessed a smooth surface with a roughness value Ra of about (0.2±0.03) μm, whereas the treated surface owed an uneven surface with a roughness value Ra of about (2.6±0.4) μm. The subsequent cellular experiment showed that the seeded osteoblasts on SMAT-treated surface spread much wider and proliferated more rapidly compared with the untreated surface. Our study suggests that SMAT could improve the living condition of osteoblast on TLM alloy surface, thus providing a promising means for the design of more potential biomedical titanium implants used on clinic.
|
|
Published: 04 May 2018
|
|
|
|
|
1 Geetha M, Singh A K, Asokamani R, et al. Ti based biomaterials, the ultimate choice for orthopaedic implants—A review[J]. Prog Mater Sci,2009,54:397.
2 Ratner B D, Bryant S J. Biomaterials: Where we have been and where we are going[J]. Annu Rev Biomed Eng,2004,6:41.
3 Alm J J, Moritz N, Aro H T. In vitro osteogenic capacity of bone marrow MSCs from postmenopausal women reflect the osseointegration of their cementless hip stems[J]. Bone Rep,2016,5:124.
4 Pilliar R M. Powder metal-made orthopedic implants with porous surface for fixation by tissue ingrowth[J]. Clin Orthop Relat Res,1983,176:42.
5 Challa V S A, Mali S, Misra R D K. Reduced toxicity and superior cellular response of preosteoblasts to Ti-6Al-7Nb alloy and comparison with Ti-6Al-4V[J]. J Biomed Mater Res A, 2013,101:2083.
6 Liu J, Wang X D, Jin Q M, et al. The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings[J]. Biomaterials,2012,33:5036.
7 Huang R, Han Y, Lu S M. Enhanced osteoblast functions and bactericidal effect of Ca and Ag dual-ion implanted surface layers on nanograined titanium alloy[J]. J Mater Chem B,2014, 2:4531.
8 Yu Z T, Zhou L. Influence of martensitic transformation on mecha-nical compatibility of biomedical β type titanium alloy TLM[J]. Mater Sci Eng A,2006,438-440:391.
9 Anselme. Osteoblast adhesion on biomaterials[J]. Biomaterials,2000,21:667.
10 Huang R, Lu S M, Han Y. Role of grain size in the regulation of osteoblast response to Ti-25Nb-3Mo-3Zr-2Sn alloy[J]. Colloids Surf B: Biointerfaces,2013,111:232.
11 Boyan B D, Sylvia V L, Liu Y H, et al. Surface roughness mediates its effects on osteoblasts via protein kinase A and phospholipase A2[J]. Biomaterials,1999,20:2305.
12 Martin J Y, Schwartz Z, Hijmmert W, et al. Bone healing after bone marrow stromal cell transplantation to the bone defect[J]. Biomaterials,1993,14(1):115.
13 Wu Y, Zitelli J P, Tenhuisen K S, et al. Differential response of Staphylococci and osteoblasts to varying titanium surface roughness[J]. Biomaterials,2011,32:951. |
|
|
|
渝公网安备50019002502923号 © Editorial Office of Materials Reports.
2017, Vol. 31 
