胶原蛋白促进成骨细胞在磷灰石基质上增殖和分化

doi:10.11896/cldb.23050130

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Abstract Bone matrix is mainly composed of type I collagen and apatite and undergoes renewal during remodeling. During this process, reversal cells recondition the resorbed surfaces left by osteoclasts and deposit a thin layer of proteins (mainly collagen) to form reversal lines, this is an obligatory step in the link between bone resorption and formation. The collagen layer is critical for osteoblast growth and the formation of new bone matrix. In order to verify the hypothesis, we modified a widely accepted simulated body fluid by increasing the relative proportion of Ca²⁺ and HPO₄^2- to effectively generate a continuous bone-like apatite substrate. Then the substrates with or without collagen pre-coating were used for MC3T3-E1 osteoblast-like cells culturing and inducing them to differentiate. The cell attachment, proliferation, and the expression of two osteogenic markers were detected. The results indicated that osteoblasts on an apatite substrate showed retracted morphologies and delayed proliferation and differentiation, and tended to secrete more collagen before they were committed to differentiation. After collagen coating of apatite substrates, osteoblasts maintained spreading morphology and higher proliferation rates, and expressed high levels of alkaline phosphatase after differentiation induction. This most probably indicated that the collagen pre-coated on apatite substrates, as deposited on the resorbed surface by reversal cells during bone remodeling, is important for osteoblast attachment, proliferation, and differentiation.

Key words： type I collagen apatite osteoblast cell spreading cell proliferation cell differentiation

Published: Online: 2024-06-25

CLC number:

Q24

Fund:Ningbo Natural Science Foundation of China (202003N4047) and the Key R & D Project in Shaanxi Province of China (2023-YBSF-302).

Corresponding Authors: *Li Ren,an associate professor in School of Life Sciences at Northwestern Polytechnical University,China.She received her B.S.,M.S.and Ph.D from Northwestern A & F University,China in 2006,2009 and 2012,respectively.In 2012,Dr.Ren started her post-doctoral research at Northwestern Polytechnical University,China.In 2015,Dr.Ren started her career at Northwestern Polytechnical University,China as lecturer and rose to associated professor in 2016.Her research focuses on microfluidic devices with biological and biomedical applications,especially in biosensors and organ-on-chips.She is an author on more than 40 peer-reviewed journal articles.renli@nwpu.edu.cn

About author: Pengyi Ma is an undergraduate in Northwestern Polytechnical University,China.His major is biotechnology.He conducted research under the guidance of professor Li Ren.His research focuses on microfluidic-based organ-on-a-chip platforms.

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Cite this article:

MA Pengyi,LI Chen,Ouaskioud Oumaima, et al. Collagen Coating Improves the Bioactivity of Apatite Substrate in Osteoblast Proliferation and Differentiation[J]. Materials Reports, 2024, 38(11): 23050130-11.

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http://www.mater-rep.com/EN/10.11896/cldb.23050130 OR http://www.mater-rep.com/EN/Y2024/V38/I11/23050130

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