基于纳米孔的单分子检测技术及其研究进展

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Abstract Nanopores have received wide attention due to their nanoscale properties. Nanopore analysis opens a new door to molecular biology investigation at the single-molecule scale as a rapid, label-free and amplification-free technique. With the application of an external voltage, molecules with sizes slightly smaller than the pore size are passed through the pore. The properties of the target molecules can be calculated by monitoring the ionic current modulations from translocation events. Single-molecule detection technologies based on nanopores have got a fast deve-lopment, have great potential applications in many areas, including nucleotides, DNA, drugs, polymers and peptides, as well as in medical diagnosis and DNA sequencing. This review mainly describes the basic principle of nanopores detection technology, several good biological nano-pores, several widely used solid-state nanopores. The advantage and disadvantage of two categories nanopores are compared and the application progress of this technology in disease diagnosis, DNA sequencing are discussed.

Key words： nanopores single molecule detection DNA sequencing translation

Published: 08 January 2021

CLC number:

Q819

Fund:This work was financially supported by the National Science and Technology Major Project of China During the 13th Five-year Plan Period (2017ZX10202101-001-016, 2018ZX10302104), the Unit Capacity Enhancement Project of Chongqing Health Commission (2019NLTS003).

About author:: Sen Yang received his master's degree in medicine from Southwest University in 2015. He is currently working at Chongqing Public Health Medical Center. His research has focused on the real-time label-free measurement of HIV-1 antibody with a solid-state nanopore sensor.Yaokai Chen received his B.S. degree in biomedicine from Kaifeng Medical College in 1987, received his M.D. degree in infectious diseases from Xi’an Medical University in 1991, and received his Ph.D. degree in bioengineering from Huazhong University of Science and Technology in 1998. He completed the postdoctoral research in infectious diseases at Third Military Medical University in 2002. After he performed research on HIV/AIDS as a visiting scholar at Yale University Medical College, he is currently a professor and consultant doctor in infectious diseases at Southwest Hospital of Third Military Medical University previously and Chongqing Public Health Medical Center. He has nearly 20 years of experience conducting clinical trial research in hospital-based settings in China, using survey research, experimental designs, randomized trials and meta analysis in topics related to HBV, HIV and relevant co-morbidities in China. His research interests are qualitative and quantitative approaches to understand the efficacy and safety of antiretroviral regimens, risk factors influencing in-hospital mortality and the extent of psychological problems in people living with HIV. He has published extensively on these topics (over 120 scholarly papers in total), and have received several grants in HBV and HIV research. He serves on the editorial board of four public health/HIV journals, and is a standing member of over ten academic associations and societies in HIV/AIDS.

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	YANG Sen
	LU Yanqiu
	SUN Feng
	CHEN Yaokai

Cite this article:

YANG Sen,LU Yanqiu,SUN Feng, et al. Nanopores-based Single Molecule Analysis Technology and Its Recent Progress[J]. Materials Reports, 2020, 34(Z2): 177-181.

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http://www.mater-rep.com/EN/ OR http://www.mater-rep.com/EN/Y2020/V34/IZ2/177

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