二肉豆蔻酰磷脂酰胆碱修饰的氧化铁纳米粒子在PC-12细胞内的分布

doi:10.11896/cldb.201906025

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Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) coated with organics have been increasingly used in biomedical research in recent years because of their good aqueous dispersibility and biocompatibility, which is essential for nanoparticles to enter cells. SPIONs coated with polyethylene glycol (PEG) and polyethyleneimine (PEI) (PEG/PEI-SPIONs) were synthesized by high temperature thermal decomposition method, then PEG/PEI-SPIONs were modified with 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) by hydrogen bonding interactions to obtain DMPC-SPIONs, and thermogravimetric analysis showed that the mass fraction of DMPC on the surface of SPIONs was 31.7wt%. The research on the uptake of PEG/PEI-SPIONs and DMPC-SPIONs by PC-12 cells showed that much more DMPC-SPIONs entered the cells, whereas relatively fewer PEG/PEI-SPIONs were observed in the cells. The results showed that DMPC played an important role in increasing the entry of SPIONs into cells. DMPC-SPIONs were densely distributed in the lysosomes, mitochondria, endoplasmic reticulum and around the nuclei in PC-12 cells. Some DMPC-SPIONs remained in the vicinity of cilia. The endocytosis process of DMPC-SPIONs was observed. DMPC-SPIONs have great potential to be used as hyperthermia agents, MRI contrast agents and drug transportation carriers.

Key words： superparamagnetic iron oxide nanoparticles (SPIONs) modification 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) cellular distribution TEM morphology

Published: 03 April 2019

CLC number:

TB332

Fund:This work was financially supported by the National Natural Science Foundation of China (51562007).

About author:: Guihua Han is studying for her M.S. degree in College of Materials Science and Engineering, Guilin University of Technology till July 2019. Baolin Zhang received his Ph.D. degree in materials sciences from Shanghai Institute of Ceramics, Chinese Academy of Sciences in 2000.

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	HAN Guihua
	ZHANG Baolin
	SU Lichao
	HUANG Yinping
	FAN Ziliang
	ZHAO Yingzheng

Cite this article:

HAN Guihua,ZHANG Baolin,SU Lichao, et al. Cellular Distribution of 1,2-dimyristoyl-sn-glycero-3-phosphocholine Modified Iron Oxide Nanoparticles[J]. Materials Reports, 2019, 33(6): 1047-1051.

URL:

http://www.mater-rep.com/EN/10.11896/cldb.201906025 OR http://www.mater-rep.com/EN/Y2019/V33/I6/1047

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