基于共价固定高密度透明质酸构建具有抗菌抗凝血双功能的表面

doi:10.11896/cldb.19040111

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Abstract Thrombosis and infections are the two major clinical complications that lead to the failure of blood-contacting biomaterials, such as in vitro blood circulation and indwelling devices. Grafting of bio-functional molecules is one of the important methods for surface modification of biomaterials. However, existing methods to solve these two complications are often based on complex modification strategies and a series of adverse reactions, for example, the commonly used silver-containing antibacterial strategy exhibits undesired hemocompatibility. Therefore, improving the antibacterial and anticoagulant properties of blood-contacting materials by simply grafting biomolecules is challenging and significant. In this study, poly(allylamine) (PAa) modified poly(dopamine) (PDA) through Schiff alkali reaction and Michael addition in alkaline condition to construct a rich-amine coating (PADA). HA was covalently immobilized to PADA based on amine coupling. The functional coating (HA-PADA) obtained both anticoagulant and antibacterial properties due to the water binding capacity and antifouling property of HA. The successful preparation of the HA-PADA coating was confirmed by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results of the adhesion and activation of fibrinogen and platelets and the antibacterial test on the surfaces confirmed that the HA-PADA coatings can significantly inhibit coagulation and bacterial adhesion. We anticipate that the dual-functional HA-PADA coating strategy with stable and ultra-high HA density would be a milestone in the development of surface engineering, especially to that of blood-contacting biomedical devices.

Key words： hyaluronic acid surface modification anticoagulant antibacterial

Published: 25 April 2020

CLC number:	TB34
	TB324
	R318.08

Fund:This work was financially supported by the National Natural Science Foundation of China (81330031).

About author:: Wenmei Mais now pursuing her master’s degree in the School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan. Her research interest is the surface engineering of blood-contacting biomedical devices.
Nan Huangis a full professor at Southwest Jiaotong University. He received his master’s degree in materials science from Southwest Jiaotong University in 1985, and worked as research fellow and Guest Professor in University of Erlangen, Germany and Rossendorf Research Center, Germany from 1989—1991 and 1998—1999 respectively. His research interests include surface and interface of biomaterials, biodegradable biomaterials, cardiovascular devices. He is an inventor of a stent which has been applied in clinic.

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MA Wenmei,HUANG Nan,XIONG Kaiqin. Construction of Surface with Anticoagulant and Antibacterial Functions via a Covalent Immobilization of Hyaluronic Acid with High Density[J]. Materials Reports, 2020, 34(8): 8165-8171.

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http://www.mater-rep.com/EN/10.11896/cldb.19040111 OR http://www.mater-rep.com/EN/Y2020/V34/I8/8165

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