| METALS AND METAL MATRIX COMPOSITES |
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| Naked-eye Detection of Hepatitis B Surface Antigen Based on Catalase-Mediated Crosslinking Aggregation of Gold Nanoparticles |
| CHEN Rubing1, HU Yongqin1,2, CHEN Meizhu1, AN Jia1,2, LYU Ying1, LIU Yufei1,2, LI Dongling1
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1 Key Laboratory of Optoelectronic Technology & Systems, Ministry of Education, Chongqing University, Chongqing 400044, China
2 Center for Intelligent Sensing Technology (CIST), College of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China |
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Abstract Developing efficient, highly sensitive, simple, stable, and low-cost virus markers detection productsfor local clinics is of great emphasis in the early diagnosis and treatment of the virus. In this paper, the bifunctional polystyrene nanospheres with both catalytic and specific recognition capabilities are prepared to catalyze the decomposition of H2O2, and the remaining H2O2 will induce the aggregation of gold nanoparticles through the crosslinking agents, which causes the color of the solution to change from red to blue, so as to achieve the high-efficiency, high-sensitivity and high-specificity naked-eye detection of hepatitis B surface antigen. The detection limit of the naked-eye was recorded as 0.5 ng/mL and the detection limit assisted with microplate reader is 0.01 ng/mL. In addition, the recovery rate of HBsAg in serum is between 88.17%—102.50%, and the relative standard deviation is between 2.01%—4.52%, indicating that the proposed immunoassay is suitable for the detection in serum and has great potential in actual clinical practice.
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Published: 10 March 2022
Online: 2022-03-08
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| Fund:Fundamental Research Funds for the Central Universities of China (2019CDQYGD020,2019CDCGGD304), the National Key Research and Development Program of China (2016YFE0125200) and the National Natural Science Foundation of China (61927818, 62005030). |
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2022, Vol. 36 
