大斯托克斯位移荧光染料研究进展

doi:10.11896/cldb.24060129

材料导报

2025, Vol. 39

Issue (14): 24060129-8 https://doi.org/10.11896/cldb.24060129

高分子与聚合物基复合材料

大斯托克斯位移荧光染料研究进展

郑森哲^1,3, 董文坤^2,3, 陈东^2,3, 凌世生^2,3, 乔旭升^1,3,*, 樊先平^1,3

1 浙江大学材料科学与工程学院硅及先进半导体材料全国重点实验室,杭州 310027
2 杭州安旭生物科技有限公司,杭州 310011
3 浙江大学-安旭生物联合研发中心,杭州 310058

Research Progress on Large Stokes Shift Fluorescent Dyes

ZHENG Senzhe^1,3, DONG Wenkun^2,3, CHEN Dong^2,3, LING Shisheng^2,3, QIAO Xusheng^1,3,*, FAN Xianping^1,3

1 School of Materials Science and Engineering & State Key Laboratory of Silicon and Advanced Semiconductor Materials, Zhejiang University, Hangzhou 310027, China
2 Assure Tech.(Hangzhou) Co., Ltd., Hangzhou 310011, China
3 ZJU-ASSURE Research & Development Center, Hangzhou 310058, China

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摘要染料作为一种重要的荧光材料,被广泛应用于目标物的标记与染色。然而,常用荧光染料的斯托克斯位移往往很小,其荧光信号容易受到背景激发光与染料自吸收的的干扰。过去的几十年间,许多大斯托克斯位移荧光染料被设计、合成和应用,多种扩大斯托克斯位移的机理被提出、发展和验证。本文综述了四种增大斯托克斯位移的机理,并梳理出基于三类不同骨架的大斯托克斯位移荧光染料,从引入取代基与扩大共轭体系的角度分析了大斯托克斯位移的来源。最后,总结了大斯托克斯位移荧光染料的研究进展,并以具有代表性的荧光染料为例,分析基于不同机理的大斯托克斯位移荧光染料分子的构建思路与光物理过程。

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	郑森哲
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	樊先平

关键词: 荧光染料大斯托克斯位移激发态分子内质子转移分子内电荷转移能量转移

Abstract: As an important class of fluorescent materials,fluorescent dyes are widely used to label and stain target in a great variety of fields. However, commercially available fluorescent dyes often exhibit very small Stokes shifts, resulting in their fluorescent signal easily influenced by high background interference and self-absorption. Over the past few decades, abundant fluorescent dyes with large Stokes shifts have been synthesized and various mechanisms to extend the Stokes shift have also been developed and implemented. In this paper, we reviewed four mechanisms to enlarge Stokes shift and three types of large Stokes shift fluorescent dyes based on different frameworks. The sources of large Stokes shift are attributed to the substituent groups and the expanded conjugation system. Finally, the research progress to enlarge Stokes shift is introduced while analyzing the ideas to design molecule and photophysical processes based on different mechanisms with representative fluorescent dyes.

Key words: fluorescent dye large Stokes shift excited-state intramolecular proton transfer intramolecular charge transfer energy transfer

出版日期: 2025-07-25 发布日期: 2025-07-29

ZTFLH:

TQ617.3

基金资助: 浙江省“领雁”研发攻关计划(2022C01142)

通讯作者: * 乔旭升,博士,浙江大学材料科学与工程学院副教授,博士研究生导师。目前主要从事发光玻璃与玻璃陶瓷、生物荧光标记材料与材料计算等领域的教学与科研工作。qiaoxus@zju.edu.cn

作者简介: 郑森哲,浙江大学材料科学与工程学院硕士研究生。目前主要研究领域为近红外大斯托克斯位移荧光染料。

引用本文:

郑森哲, 董文坤, 陈东, 凌世生, 乔旭升, 樊先平. 大斯托克斯位移荧光染料研究进展[J]. 材料导报, 2025, 39(14): 24060129-8.
ZHENG Senzhe, DONG Wenkun, CHEN Dong, LING Shisheng, QIAO Xusheng, FAN Xianping. Research Progress on Large Stokes Shift Fluorescent Dyes. Materials Reports, 2025, 39(14): 24060129-8.

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https://www.mater-rep.com/CN/10.11896/cldb.24060129 或 https://www.mater-rep.com/CN/Y2025/V39/I14/24060129

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