蚕丝在生物医用材料领域的应用研究

doi:10.11896/j.issn.1005-023X.2018.01.010

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Abstract

Silk is an excellent natural protein fiber as well as a high-quality polymer protein material with good mechanical properties, biocompatibility, controllable biodegradability, etc. With the gradual development of biomaterial field and the cross-integration of various disciplines, silk as a biomaterial has shown the strong competitiveness and exhibited great application potential in this field. In this paper, the composition and characteristics of silk are introduced, and the extracting methods for both silk fibroin and sericin are also summarized. Furthermore, the applications of silk and silk proteins in tissue engineering, drug loading and wound dressing in recent years are reviewed. Besides, the functions of silk and silk proteins for specific applications as well as the advantages and disadvantages of silk-based material are analyzed. Finally, the application prospect of silk in biomaterial field is proposed.

Key words： silk silk proteins biomaterials biomedical application

Published: 10 January 2018

CLC number:

TS102.3

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	Articles by authors
	Yan MA
	Zhi LI
	Ruilong RAN
	Kang LI

Cite this article:

Yan MA,Zhi LI,Ruilong RAN, et al. Research on Application of Silk in Biomaterial Field[J]. Materials Reports, 2018, 32(1): 86-92.

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http://www.mater-rep.com/EN/10.11896/j.issn.1005-023X.2018.01.010 OR http://www.mater-rep.com/EN/Y2018/V32/I1/86

The SEM (a) morphology and (b) inner structure of silk

Degumming methods of silk and their characteristics

Extraction methods for sericin and their characteristics

Application of silk in biomaterial field

[1]	Vepari C, Kaplan D L . Silk as a biomaterial[J]. Progress in Polymer Science, 2007,32(8-9):991.
[2]	Jin H J, Kaplan D L . Mechanism of silk processing in insects and spiders[J]. Nature, 2003,424(6952):1057.
[3]	Omenetto F G, Kaplan D L . New opportunities for an ancient material[J]. Science, 2010,329(5991):528.
[4]	Perrone G S, Leisk G G, Lo T J , et al. The use of silk-based devices for fracture fixation[J]. Nature Communications, 2014,5(3):3385.
[5]	Das S, Sharma M, Saharia D , et al. In vivo studies of silk based gold nano-composite conduits for functional peripheral nerve regeneration[J]. Biomaterials, 2015,62:66.
[6]	Guziewicz N A, Massetti A J , Perez-Ramirez B J, et al. Mechanisms of monoclonal antibody stabilization and release from silk biomaterials[J]. Biomaterials, 2013,34(31):7766.
[7]	Lerdchai K, Kitsongsermthon J, Ratanavaraporn J , et al. Thai silk fibroin/gelatin sponges for the dual controlled release of curcumin and docosahexaenoic acid for anticancer treatment[J]. Journal of Pharmaceutical Sciences, 2016,105(1):221.
[8]	Silva R, Fabry B, Boccaccini A R . Fibrous protein-based hydrogels for cell encapsulation[J]. Biomaterials, 2014,35(25):6727.
[9]	Cai Z X, Mo X M, Zhang K H , et al. Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications[J]. International Journal of Molecular Sciences, 2010,11(9):3529.
[10]	MinouraN, Aiba S, Higuchi M , et al. Attachment and growth of fibroblast cells on silk fibroin[J]. Biochemical and Biophysical Research Communications, 1995,208(2):511.
[11]	LinS, Chen M, Jiang H , et al. Green electrospun grape seed extract-loaded silk fibroin nanofibrous mats with excellent cytocompatibility and antioxidant effect[J]. Colloids and Surfaces B-Biointerfaces, 2016,139(1):56.
[12]	WeiY L, Sun D, Yi H G , et al. Characterization of a PEG-DE cross-linked tubular silk scaffold[J]. Textile Research Journal, 2014,84(9):959.
[13]	ZhouW, Feng Y, Yang J , et al. Electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for endothelial cell growth[J]. Journal of Materials Science-Materials in Medicine, 2015,26(1):5386.
[14]	ErselM, Uyanikgil Y, Karbek Akarca F , et al. Effects of silk sericin on incision wound healing in a dorsal skin flap wound healing rat model[J]. Medical Science Monitor, 2016,22:1064.
[15]	GuanG, Wang L, Li M , et al. In vivo biodegradation of porous silk fibroin films implanted beneath the skin and muscle of the rat[J]. Biomedical Materials and Engineering, 2014,24(1):789.
[16]	LeeO J, Lee J M, Kim J H , et al. Biodegradation behavior of silk fibroin membranes in repairing tympanic membrane perforations[J]. Journal of Biomedical Materials Research Part A, 2012,100(8):2018.
[17]	LuQ, Zhang B, Li M , et al. Degradation mechanism and control of silk fibroin[J]. Biomacromolecules, 2011,12(4):1080.
[18]	GoslineJ M, Guerette P A, Ortlepp C S , et al. The mechanical design of spider silks: From fibroin sequence to mechanical function[J]. Journal of Experimental Biology, 1999,202(23):3295.
[19]	JiangC Y, Wang X Y, Gunawidjaja R , et al. Mechanical properties of robust ultrathin silk fibroin films[J]. Advanced Functional Materials, 2007,17(13):2229.
[20]	Ma Y, Li Z, Dai F Y , et al. A review on modification of silk and silk fabrics[J].Science of Sericulture, 2016(6):1106 (in Chinese).
[20]	马艳, 李智, 代方银 , 等. 蚕丝及蚕丝织物的改性研究综述[J].蚕业科学, 2016(6):1106.
[21]	ZhouX J, Dong X, An analysis on the influence of different degumming methods on silk properties [J]. Knitting Industries, 2013(4):44 (in Chinese).
[21]	周小进, 董雪 . 不同脱胶方法对蚕丝性能的影响分析>[J].针织工业, 2013(4):44.
[22]	HouA Q,, Shi Y Q, Xie K L, Extraction of silk fibroin and its application in textiles[J].Knitting Industries, 2009(4):63(in Chinese).
[22]	侯爱芹, 史雅琪, 谢孔良 . 丝素蛋白的提取及其在纺织上的应用进展[J].针织工业, 2009(4):63.
[23]	HuangX S . Comparison and analysis of several degumming methods for silkworm cocoon extraction Guangxi Sericulture, 2009,46(4):1(in Chinese).
[23]	黄贤帅 . 桑蚕削口茧几种脱胶方法的比较分析[J]. 广西蚕业, 2009,46(4):1.
[24]	ZhangY Q . Comparative analysis of silk degumming methods Science of Sericulture, 2002,28(1):75(in Chinese).
[24]	张雨青 . 蚕丝脱胶方法的比较分析[J]. 蚕业科学, 2002,28(1):75.
[25]	YangM, Shuai Y, He W , et al. Preparation of porous scaffolds from silk fibroin extracted from the silk gland of Bombyx mori (B. mori)[J]. International Journal of Molecular Sciences, 2012,13(6):7762.
[26]	MaJ, Wang X Y, Li Q , et al. Study on methods of silk sericin recovery Journal of Anhui Agricultural Sciences, 2015,33(4):674(in Chinese).
[26]	马骏, 王学英, 李群 , 等. 蚕丝丝胶蛋白提取方法的研究[J]. 安徽农业科学, 2005,33(4):674.
[27]	YeC J, Li B, Meng Y , et al. Extraction of natural sericin and screening of its enzymatic hydrolysis conditions China Sericulture, 2015,36(4):44(in Chinese).
[27]	叶崇军, 李冰, 孟艳 , 等. 全天然丝胶蛋白的提取及酶解条件的筛选[J]. 中国蚕业, 2015,36(4):44.
[28]	MeinelL, Fajardo R, Hofmann S , et al. Silk implants for the healing of critical size bone defects[J]. Bone, 2005,37(5):688.
[29]	RajkhowaR, Gil E S, Kluge J , et al. Reinforcing silk scaffolds with silk particles[J]. Macromolecular Bioscience, 2010,10(6):599.
[30]	MandalB B, Grinberg A, Gil E S , et al. High-strength silk protein scaffolds for bone repair[J]. Proceedings of The National Academy of Sciences of The United States of America USA, 2012,109(20):7699.
[31]	KuboyamaN, Kiba H, Arai K , et al. Silk fibroin-based scaffolds for bone regeneration[J]. Journal of biomedical materials research Part B-Applied Biomaterials, 2013,101(2):295.
[32]	FossC, Merzari E, Migliaresi C , et al. Silk fibroin/hyaluronic acid 3D matrices for cartilage tissue engineering[J]. Biomacromolecules, 2013,14(1):38.
[33]	YamamotoK, Tomita N, Fukuda Y , et al. Time-dependent changes in adhesive force between chondrocytes and silk fibroin substrate[J]. Biomaterials, 2007,28(10):1838.
[34]	LiX, He J, Bian W , et al. A novel silk-based artificial ligament and tricalcium phosphate/polyether ether ketone anchor for anterior cruciate ligament reconstruction-safety and efficacy in a porcine model[J]. Acta Biomaterialia, 2014,10(8):3696.
[35]	LiX, He J K, Bian W G , et al. A novel silk-TCP-PEEK construct for anterior cruciate ligament reconstruction:An off the shelf alternative to a bone-tendon-bone autograft[J]. Biofabrication, 2014,6(1):015010.
[36]	VachirarojN, Ratanavaraporn J, Damrongsakkul S , et al. A comparison of Thai silk fibroin-based and chitosan-based materials on in vitro biocompatibility for bone substitutes[J]. International Journal of Biological Macromolecules, 2009,45(5):470.
[37]	WangL, Lu C X, Li Y H , et al. Green fabrication of porous silk fibroin/graphene oxide hybrid scaffolds for bone tissue engineering[J]. RSC Advances, 2015,5(96):78660.
[38]	ShiL Y . Preparation and properties of hydroxyapatite-silk firoin/chitosan composite scaffolds[D]. Hangzhou:Zhejiang University, 2014(in Chinese).
[38]	施李杨 . 羟基磷灰石-丝素/壳聚糖复合支架的制备及性能研究[D]. 杭州: 浙江大学, 2014.
[39]	AltmanG H, Horan R L, Lu H H , et al. Silk matrix for tissue engineered anterior cruciate ligaments[J]. Biomaterials, 2002,23(20):4131.
[40]	ShenW, Chen X, Chen J , et al. The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration[J]. Biomaterials, 2010,31(28):7239.
[41]	ChenX, Qi Y Y, Wang L L , et al. Ligament regeneration using a knitted silk scaffold combined with collagen matrix[J]. Biomaterials, 2008,29(27):3683.
[42]	FanH, Liu H, Wong E J , et al. In vivo study of anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold[J]. Biomaterials, 2008,29(23):3324.
[43]	LiuH, Ge Z, Wang Y , et al. Modification of sericin-free silk fibers for ligament tissue engineering application[J]. Journal of Biomedical Materials Research Part B-Applied Biomaterials, 2007,82(1):129.
[44]	ShiP, Teh T K, Toh S L , et al. Variation of the effect of calcium phosphate enhancement of implanted silk fibroin ligament bone integration[J]. Biomaterials, 2013,34(24):5947.
[45]	YagiT, Sato M, Nakazawa Y , et al. Preparation of double-raschel knitted silk vascular grafts and evaluation of short-term function in a rat abdominal aorta[J]. Journal of Artificial Organs, 2011,14(2):89.
[46]	WangJ W, Li X D, Wei G F . Cell co-culture technology accelerates premicrovascular-like structure formation Journal of Clinical Rehabilitative Tissue Engineering Research, 2013,17(38):6779(in Chinese).
[46]	王纪文, 李向东, 魏国峰 . 细胞共培养技术促进类前微血管结构发生[J]. 中国组织工程研究, 2013,17(38):6779.
[47]	LiuH, Li X, Zhou G , et al. Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering[J]. Biomaterials, 2011,32(15):3784.
[48]	YanS Q, Zhang Q, Wang J N , et al. Silk fibroin/chondroitin sulfate/hyaluronic acid ternary scaffolds for dermal tissue reconstruction[J]. Acta Biomaterialia, 2013,9(6):6771.
[49]	HanF, Liu S, Liu X , et al. Woven silk fabric-reinforced silk nanofibrous scaffolds for regenerating load-bearing soft tissues[J]. Acta Biomaterialia, 2014,10(2):921.
[50]	HanF, Li H B, Li L H, Qian Y N , et al. Preparation and cytocompatibility study of poly(ε-caprolactone)/silk sericin nanofibrous scaffolds[J]. Journal of Biomedical Engineering, 2011(2):305 (in Chinese).
[50]	李海滨, 李林昊, 钱宇娜 , 等. PCL/SS纳米纤维支架的制备及相容性研究[J].生物医学工程学杂志, 2011(2):305.
[51]	YooC R, Yeo I S, Park K E , et al. Effect of chitin/silk fibroin nanofibrous bicomponent structures on interaction with human epidermal keratinocytes[J]. International Journal of Biological Macromolecules, 2008,42(4):324.
[52]	NayakS, Dey S, Kundu S C . Skin equivalent tissue-engineered construct: Co-cultured fibroblasts/keratino-cytes on 3D matrices of sericin hope cocoons[J]. Plos One, 2013,8(9):e74779.
[53]	XuY, Zhang Z, Chen X , et al. A silk fibroin/collagen nerve scaffold seeded with a co-culture of schwann cells and adipose-derived stem cells for sciatic nerve regeneration[J]. Plos One, 2016,11(1):e0147184.
[54]	LiG, Kong Y, Zhao Y , et al. Fabrication and characterization of polyacrylamide/silk fibroin hydrogels for peripheral nerve regeneration[J]. Journal of Biomaterials Science-Polymer Edition, 2015,26(14):899.
[55]	WeiY, Gong K, Zheng Z , et al. Chitosan/silk fibroin-based tissue-engineered graft seeded with adipose-derived stem cells enhances nerve regeneration in a rat model[J]. Journal of Materials Science-Materials in Medicine, 2011,22(8):1947.
[56]	WangC Y, Zhang K H, Fan C Y , et al. Aligned natural-synthetic polyblend nanofibers for peripheral nerve regeneration[J]. Acta Biomaterialia, 2011,7(2):634.
[57]	Maghdouri-White Y, Bowlin G L, Lemmon C A , et al. Mammary epithelial cell adhesion, viability, and infiltration on blended or coated silk fibroin-collagen type I electrospun scaffolds[J]. Materials Science & Engineering C-Materials for Biological Applications, 2014,43:37.
[58]	CritchfieldA S, McCabe R, Klebanov N , et al. Biocompatibility of a sonicated silk gel for cervical injection during pregnancy: In vivo and in vitro study[J]. Reproductive Sciences, 2014,21(10):1266.
[59]	KasojuN, Bora U . Silk fibroin based biomimetic artificial extracellular matrix for hepatic tissue engineering applications[J]. Biomedical Materials, 2012,7(4):45004.
[60]	ChungE J, Ju H W, Park H J , et al. Three-layered scaffolds for artificial esophagus using poly(epsilon-caprolactone) nanofibers and silk fibroin: An experimental study in a rat model[J]. Journal of Biomedical Materials Research Part A, 2015,103(6):2057.
[61]	GallerK M, D'Souza R N . Tissue engineering approaches for regenerative dentistry[J]. Regenerative Medicine, 2011,6(1):111.
[62]	HarkinD G, George K A, Madden P W , et al. Silk fibroin in ocular tissue reconstruction[J]. Biomaterials, 2011,32(10):2445.
[63]	XueH J, Liu L, Hu D D , et al. Fabrication and property test of chitosan/silk fibroin composite films with low swelling ratio Science of Sericulture, 2011,37(6):1073(in Chinese).
[63]	薛豪杰, 刘琳, 胡丹丹 , 等. 低溶胀壳聚糖/丝素蛋白复合膜的制备及性能测试[J]. 蚕业科学, 2011,37(6):1073.
[64]	Lu C, Zhang J, Wang Y F , et al. Research progress of the preparation methods of silk fibroin microsphere[J].Progress in Textile Science & Technology, 2016(9):1(in Chinese).
[64]	卢晨, 张捷, 王亚飞 , 等. 蚕丝蛋白微球制备方法的研究进展[J].纺织科技进展, 2016(9):1.
[65]	GuZ P, Xie H X, Huang C C , et al. Preparation of chitosan/silk fibroin blending membrane fixed with alginate dialdehyde for wound dressing[J]. International Journal of Biological Macromolecules, 2013,58:121.
[66]	HouZ M . Study on preparation and properties of chitin / silk fibroin composite medical biological dressing[D]. Zhejiang Sci-Tech University, 2008,25(2):141(in Chinese).
[66]	侯智谋 . 甲壳素/丝蛋白纤维复合医用生物敷料的制备及性能研究[J]. 浙江理工大学学报, 2008,25(2):141.
[67]	InpanyaP, Faikrua A, Ounaroon A , et al. Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats[J]. Biomedical Materials, 2012,7(3):35008.
[68]	ShahverdiS, Hajimiri M, Esfandiari M A , et al. Fabrication and structure analysis of poly(lactide-co-glycolic acid)/silk fibroin hybrid scaffold for wound dressing applications[J]. International Journal of Pharmaceutics, 2014,473(1-2):345.
[69]	KanokpanontS, Damrongsakkul S, Ratanavaraporn J , et al. An innovative bi-layered wound dressing made of silk and gelatin for accelerated wound healing[J]. International Journal of Pharmaceutics, 2012,436(1-2):141.
[70]	KanokpanontS, Damrongsakkul S, Ratanavaraporn J , et al. Physico-chemical properties and efficacy of silk fibroin fabric coated with different waxes as wound dressing[J]. International Journal of Biological Macromolecules, 2013,55(2):88.
[71]	LiL H . Fabricaion of electrospun silk protein based composite nanofibers and their relevant applicaion[D]. Chongqing:Chongqing University, 2015(in Chinese).
[71]	李林昊 . 基于蚕丝蛋白静电纺丝复合纳米纤维的构建及其相关应用研究[D]. 重庆:重庆大学, 2015.
[72]	ChenL, Zhu Y B, Li Y Y , et al. Progress and prospect of electrospun silk fibroin in construction of tissue-engineering scaffold Chinese Journal of Biotechnology, 2011,27(6):831(in Chinese).
[72]	陈玲, 竺亚斌, 李媛媛 , 等. 丝素蛋白在电纺丝法构建组织工程支架中的应用进展[J]. 生物工程学报, 2011,27(6):831.
[73]	LiS, Shi X Y, Lin W , et al. Repairing peripheral nerve defects in rats by electrostatic spinning poly (lactic-co-glycolic acid)-silk fibroin-collagen nerve conduits Chinese Journal of Tissue Engineering Research, 2012,16(38):7087(in Chinese).
[73]	李森, 史晓远, 林卫 , 等. 静电纺丝聚乳酸聚乙醇酸共聚物-丝素-胶原神经导管修复大鼠周围神经缺损[J]. 中国组织工程究, 2012,16(38):7087.
[74]	CaiJ Y, Wang C Y, Fan C Y . Progress in the study of nerve conduits containing silk fibroin in nerve tissue engineering International Journal of Orthopaedics, 2015,36(6):394 (in Chinese).
[74]	蔡江瑜, 汪春阳, 范存义 . 含丝素蛋白的神经导管在神经组织工程中的研究进展[J]. 国际骨科学杂志, 2015,36(6):394.
[75]	MandalB B, Kundu S C . Calcium alginate beads embedded in silk fibroin as 3D dual drug releasing scaffolds[J]. Biomaterials, 2009,30(28):5170.
[76]	XieM B, Fan D, Chen Y F , et al. An implantable and controlled drug-release silk fibroin nanofibrous matrix to advance the treatment of solid tumour cancers[J]. Biomaterials, 2016,103:33.