Materials Reports  2019, Vol. 33 Issue (Z2): 547-552 |
| POLYMERS AND POLYMER MATRIX COMPOSITES |
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| Reductive-responsive Albumin Nanoparticle Loading Methotrexate for Anti-cancer Therapy |
| WANG Huaiji, DONG Haiqing
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| The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai 200092 |
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Abstract We constructed a self-assembly albumin nanoparticle-based drug delivery system based on disulfide bond by mediating the thiol exposure of bovine serum albumin (BSA). BSA nanoparticle (BSA NP) loaded with methotrexate (MTX) was synthesized successfully which exhibited good size distribution, morphology and high zeta potential. MTX loaded BSA NP (BSA-MTX) had two absorption peaks at 280 nm and 395 nm, respectively, which proved that MTX was loaded into BSA NP successfully. The disulfide cross-linking nets within the reductive-responsive nanoparticle could be disrupted by dithiothreitol (DTT), a reductive molecule, so as to induce the dissociation of BSA-MTX to release MTX. The size of BSA-MTX experienced small change incubated with RPMI 1640 containing 10% FBS within 5 days. Moreover, BSA-MTX exhibited stronger toxicity towards B16F10 cancer cells and induced cancer cells apoptosis efficiently. Besides, BSA-MTX was biocompatible which would not cause the hemolysis in vitro. Meanwhile, BSA-MTX along with BSA NP could be endocytosed by cancer cell, an indicative of the loaded MTX hardly affecting the cell uptake efficiency of BSA NP. BSA-MTX was able to inhibit the tumor growth of the mice and significantly improve the therapeutic effect effectively. The novel albumin nanoparticle-based drug delivery system provides a new approach for cancer therapy.
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Published: 25 November 2019
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| Fund:This work was financially supported by the National Natural Science Foundation of China, General Program (31771090). |
About author:: Huaiji Wang is currently pursuing his M.S. degree at School of Medicine, Tongji University. His research interest is biomedical polymers and supramolecular self-assembly in the applications of nanomedicine and nanotechnology.
Haiqing Dong is an associate research scientist in the Institute for Biomedical Engineering & Nano Science of Tongji Univ. School of Medicine. She received her Ph. D. from College of Chemistry and Molecular Science, Wuhan University in 2009. Her interests focus on biomedical polymers and supramolecular self-assembly in the applications of nanomedicine and nanotechnology and immune cell carriers in tumor targeting and therapy. |
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