RESEARCH PAPER |
|
|
|
|
|
In Vitro Cytotoxicity Evaluation of Cellulose Absorbable Hemostatic Materials |
YU Yan1, MA Fengsen1, LU Jiajun2, CHEN Haibo2
|
1 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014; 2 Zhejiang Institute of Medical Device Testing, Hangzhou 310018 |
|
|
Abstract In order to provide reference for clinical application of cellulose absorbable hemostatic materials, cytocompatibility of three absorbable hemostatic materials (Surgicel, Yingjieer and Taikeling) were evaluated by cytotoxicity testing in vitro. The effects of the three materials on the proliferation and the cytotoxicity of L929 cells were evaluated by MTT colorimetric method, cell morphology analysis, agar diffusion method and membrane diffusion method. The incomplete culture medium was added to every material at a ratio of sample/medium of 50.00 mg/mL. After 24 h, the extracts solution designated as 100% extracts was separated by centrifugation and decantation. The extracts was serially diluted to give 50.0%, 25.0%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, and 0.39% extracts. MTT colorimetric method combined with cell morphology analysis showed that each concentration of extract of Yingjieer had no significant cytotoxicity, while extracts of Surgicel and Taikeling had no significant cytotoxicity when their concentration was below 25.0%. Among the three materials, agar diffusion method and membrane diffusion method showed that Yingjieer had no significant cytotoxicity, but Surgicel and Taikeling had significant cytotoxicity. The cytocompatibilitys of the three different hemostatic materials were different, therefore it is important to select appropriate evaluation methods.
|
Published: 25 March 2018
|
|
|
|
1 Barnard J, Millner R. A review of topical hemostatic agents for use in cardiac surgery[J].The Annals of Thoracic Surgery,2009,88(4):1377. 2 Xiong X Q, Mei X, Ma F S. Research on polysaccharide absorbable hemostatic materials[J].Chinese Journal of Trauma,2015,31(6):571(in Chinese). 熊绪琼,梅昕,马凤森.多糖类可吸收止血材料的研究进展[J].中华创伤杂志,2015,31(6):571. 3 Li Y J, Shi Y Q. Analysis of adverse event reports of Surgicel in MAUDE database[J].Chinese Journal of Pharmacovigilance,2013,10(5):312(in Chinese). 李玉基,史玉清.MAUDE数据库中速即纱不良事件报告分析[J].中国药物警戒,2013,10(5):312. 4 Kakaei F, Sadeghi M S S, Sanei B, et al. A randomized clinical trial comparing the effect of different haemostatic agents for haemostasis of the liver after hepatic resection[J].HPB Surgery,2013,2013:1. 5 Ibrahim M F, Aps C, Young C P. A foreign body reaction to Surgicel mimicking an abscess following cardiac surgery[J].European Journal of Cardio-Thoracic Surgery,2002,22(3):489. 6 Zhao B, Xiao K S, Ren Y B, et al. To evaluate the in vitro biocompatibility of phosphorus-magnesium whisker using MTT[J].Stomatology,2016,36(4):345(in Chinese). 赵冰,肖克沈,任伊宾,等.MTT法评价新型磷镁晶须的体外生物相容性研究[J].口腔医学,2016,36(4):345. 7 Zhao C Y. Biocompatibility research of the new hemostatic agent against the war wound[D].Xi’an:The Fourth Military Medical University,2010(in Chinese). 赵聪颖. 新型战伤急救止血剂的生物安全性研究[D].西安:第四军医大学,2010. 8 Shi P, Jiang J H. The research progress of oral material cell toxicity evaluation method[J].Chinese Journal of Aesthetic Medicine,2014,23(14):1222(in Chinese). 石平,蒋均红.口腔材料细胞毒性评价方法的研究进展[J].中国美容医学,2014,23(14):1222. 9 Granja P L, Jéso B D, Bareille R, et al. Cellulose phosphates as biomaterials. In vitro biocompatibility studies[J].Reactive and Functional Polymers,2006,66(7):728. 10 Hexig B, Nakaoka R, Tsuchiya T. Safety evaluation of surgical materials by cytotoxicity testing[J].Journal of Artificial Organs,2008,11(4):204. 11 Li H, Peng Q, Li X, et al. Microstructures, mechanical and cytocompatibility of degradable Mg-Zn based orthopedic biomaterials[J].Materials and Design,2014,58:43. 12 奚廷斐. 医疗器械生物学评价[M].北京:中国标准出版社,2012:115. 13 GB/T 14233.2-2005医用输液、输血、注射器具检验方法.第2部分:生物学试验方法[S]. 14 YY/T0127.9-2009口腔医疗器械生物学评价.第2单元:试验方法细胞毒性试验:琼脂扩散法及滤膜扩散法[S]. 15 Peng B, Wu J J, Li Y, et al. A methodological study of Trypan Blue exclusion test for cultured adherent cells[J].Acta Laser Biology Sinica,2011,20(2):269(in Chinese). 彭彬,吴晶晶,李烨,等.贴壁培养细胞台盼蓝拒染试验的方法学探讨[J].激光生物学报,2011,20(2):269. 16 Wang X H, Chen Y G, Yu S, et al. Effects of different concentration of Mg-6Zn alloy extracts on apoptosis and the related caspase-3 expression in intestinal epithelial cells[J].Materials Review B:Research Papers,2015,29(4):47(in Chinese). 王啸虎,陈义钢,于嵩,等.不同浓度Mg-6Zn合金浸提液对肠上皮细胞凋亡及其相关基因Caspase-3表达的影响[J].材料导报:研究篇,2015,29(4):47. 17 Zhu M Q. Reasearch on absorbable hemostatic gauze[D].Hangzhou:Zhejiang University,2014(in Chinese). 朱美琴. 可吸收止血纱布研究[D].杭州:浙江大学,2014. 18 Mao J S, Cui Y L, Wang X H, et al. A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis[J].Biomaterials,2004,25(18):3973. 19 Ma L K, Ye P, Deng J, et al. The cytotoxicity of silk fibroin/chitosan/nano hydroxyapatite bone tissue engineering scaffolds in vitro[J].Medical Journal of West China,2014,26(8):975(in Chinese). 马立坤,叶鹏,邓江,等.丝素蛋白/壳聚糖/纳米羟基磷灰石骨组织工程支架材料的体外细胞毒性评价[J].西部医学,2014,26(8):975. 20 Wang X, Shi Y P, Zhu X T, et al. Evaluation on the cytotoxicity of polymeric material for medical use by MTT-assay[J].Shangdong Journal of Biomedical Engineering,2003,22(1):46(in Chinese). 王昕,施嬿萍,朱雪涛,等.MTT法评价医用高分子材料的细胞毒性[J].山东生物医学工程,2003,22(1):46. 21 奚廷斐.医疗器械生物学评价[M].北京:中国标准出版社,2012:98. 22 Yang L J, Si X H, Wang W L, et al. Detection of apoptosis in hepatoma cells under optical microscopic observation after stained by six methods[J].Journal of Practical Medical Techniques,2006,1(1):8(in Chinese). 杨连君,司晓辉,王文亮,等.六种染色后光镜观察法检测肝癌细胞凋亡[J].实用医技杂志,2006,1(1):8. 23 Murni N S, Dambatta M S, Yeap S K, et al. Cytotoxicity evaluation of biodegradable Zn-3Mg alloy toward normal human osteoblast cells[J].Materials Science and Engineering:C,2015,49:560. 24 Li K, Zhou R, Jia W W, et al. Zanthoxylum bungeanum essential oil induces apoptosis of HaCaT human keratinocytes[J].Journal of Ethnopharmacology,2016,186:351. |
|
|
|