利用Triton X-100提升巴氏芽孢杆菌脲酶活性的效果

doi:10.11896/cldb.23060069

Abstract
Figure/Table
References
Related Citation (14)

Download:

Full Text ( PDF ) (16178KB)
Export: BibTeX | EndNote (RIS)

Abstract Microbially induced carbonate precipitation (MICP) technology is a new green reinforcement technology developed in recent years. How to improve the reinforcement efficiency of MICP has always been a focus of scholars’ attention. This work focuses on the principle of urease produced by Sporosarcina pasteurii and the characteristics of non-ionic surfactant. The experiment of improving MICP efficiency with non-ionic surfactant Triton X-100 was designed. The results showed that:(Ⅰ) based on the results of single factor experiment, response surface experiment was designed to analyze and determine the optimal treatment conditions of the surfactant, namely, the dosage was 1.37%, the treatment time was 5.02 h, the treatment temperature was 34.28 ℃, and the ratio of bacteria to cementation solution was 1∶2.11;(Ⅱ) the calcium carbonate content and unconfined compressive strength of the solidified body increased by 25.48% and 22.83% respectively after the calcareous sand was reinforced by Sporosarcina pasteurii under the optimal conditions;(Ⅲ) the non-ionic surfactant Triton X-100 increases the permeability of the cell membrane of Sporosarcina pasteurii. On the one hand, more intracellular urease is released to the outside of the cell, while more urea enters the cell, which promotes the hydrolysis of urea and the formation rate of carbonate. On the other hand, the calcium carbonate gel structure generates with bacteria as the core more compact. Under the effect of these two aspects, the MICP efficiency is effectively improved. Relevant ideas and research results can provide new reference and ideas for the improvement of MICP reinforcement effect.

Key words： microbially induced carbonate precipitation (MICP) urease non-ionic surfactant cell membrane permeability response surface method

Published: Online: 2024-01-16

CLC number:

TU521

Fund:National Natural Science Foundation of China (U22A20600,U2034203).

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	XIAO Yao
	DENG Huafeng
	LI Jianlin
	XIONG Yu
	CHENG Lei

Cite this article:

XIAO Yao,DENG Huafeng,LI Jianlin, et al. Effect of Triton X-100 on Improving Urease Activity of Sporosarcina Pasteurii[J]. Materials Reports, 2024, 38(1): 23060069-7.

URL:

http://www.mater-rep.com/EN/10.11896/cldb.23060069 OR http://www.mater-rep.com/EN/Y2024/V38/I1/23060069

1 DeJong J T, Mortensen B M, Martinez B C, et al. Ecological Enginee-ring, 2008, 36(2), 197.
2 Kantzas A, Stehmeier L, Marentette D F, et al. In:Proceedings of Annual Technical Meeting. Canada, 1992, pp. 46.
3 Yang Y, Chu J, Xiao Y, et al. Construction and Building Materials, 2019, 212, 342.
4 Li H, Tang C S, Liu B, et al. Chinese Journal of Geotechnical Enginee-ring, 2020, 42(10), 1931 (in Chinese).
李昊, 唐朝生, 刘博, 等. 岩土工程学报, 2020, 42(10), 1931.
5 Li C, Wang S, Wang Y X, et al. Rock and Soil Mechanics, 2019, 40(4), 1291 (in Chinese).
李驰, 王硕, 王燕星, 等. 岩土力学, 2019, 40(4), 1291.
6 Qian C X, Zheng T W, Zhang X, et al. Construction and Building Materials, 2021, 290, 123226.
7 Chen R F, Miao L C, Sun X H, et al. Rock and Soil Mechanics, 2020, 41(3), 933 (in Chinese).
陈润发, 缪林昌, 孙潇昊, 等. 岩土力学, 2020, 41(3), 933.
8 Li M, Cheng X H, Guo H X. International Biodeterioration & Biodegradation, 2013, 76, 81.
9 Liu S Y, Yu J, Zeng W L, et al. Chinese Journal of Rock Mechanics and Engineering, 2020, 39(1), 191 (in Chinese).
刘士雨, 俞缙, 曾伟龙, 等. 岩石力学与工程学报, 2020, 39(1), 191.
10 Bu C M, Lu X Y, Zhu D X, et al. Arabian Journal of Geosciences, 2022, 15(9), 863.
11 Yang Y, Ruan S Q, Wu S F, et al. Acta Geotechnica, 2021, 16(4), 1113.
12 Zhao Y, Fan C B, Liu P H, et al. Environmental Earth Sciences, 2018, 77(17), 615.
13 Zhang J K, Su P D, Wen K J, et al. KSCE Journal of Civil Engineering, 2020, 24(11), 3189.
14 Zhao Y, Xiao Z Y, Fan C B, et al. Bulletin of Engineering Geology and the Environment, 2020, 79(6), 3075.
15 Nafise H B, Davood M, Mohamadreza E. Construction and Building Materials, 2017, 141, 565.
16 Mo F. Optimizing the composition of phospholipids and the absence of Dalanylacylation of lipoteichoic acid to promote the high yield of heterologous protein of Bacillus licheniformis. Master’s Thesis, Hubei University, China, 2020 (in Chinese).
莫非. 磷脂组分优化及脂磷壁酸D-丙氨酰化缺失促地衣芽胞杆菌异源蛋白高产. 硕士学位论文, 湖北大学, 2020.
17 Shi G Y. Experimental study on the solidification effect of calcium carbonate deposited by urease in soil. Master’s Thesis, Inner Mongolia University of Technology, China, 2020 (in Chinese).
史冠宇. 脲酶沉积碳酸钙在土中发挥固化效用的试验研究. 硕士学位论文, 内蒙古工业大学, 2020.
18 Zou W Y, Yang X Z, Song H, et al. Detergent & Cosmetics, 2016, 39(7), 55 (in Chinese).
邹文苑, 杨许召, 宋浩, 等. 日用化学品科学, 2016, 39(7), 55.
19 Ding Y, Yuan X Z, Zeng G M, et al. Environmental Science, 2010, 31(4), 1047 (in Chinese).
丁莹, 袁兴中, 曾光明, 等. 环境科学, 2010, 31(4), 1047.
20 Seockheon L, Kweon J H, Kim H S. International Biodeterioration & Biodegradation, 2013, 85, 652.
21 Koichi T, Miura A, Koike H, et al. Journal of Biotechnology, 2017, 248, 9.
22 Liu J L, Li Z M, Zhao G Q, et al. Anhui Chemical Industry, 2019, 5(2), 30 (in Chinese).
刘金龙, 李志敏, 赵国群, 等. 安徽化工, 2019, 5(2), 30.
23 Xiao Y, Deng H F, Li J L, et al. Rock and Soil Mechanics, 2022, 43(2), 395 (in Chinese).
肖瑶, 邓华锋, 李建林, 等. 岩土力学, 2022, 43(2), 395.
24 Sun R R, Huang P X, Li W G, et al. Applied Chemical Industry, 2013, 42(1), 26 (in Chinese).
孙儒瑞, 黄品鲜, 李伟光, 等. 应用化工, 2013, 42(1), 26.
25 Peng J, Feng Q P, Sun Y C. Chinese Journal of Geotechnical Enginee-ring, 2018, 40(6), 1048 (in Chinese).
彭劼, 冯清鹏, 孙益成. 岩土工程学报, 2018, 40(6), 1048.
26 Kalil S J, Maugeri F, Rodrigues M I. Process Biochemistry, 2000, 35(6), 539.
27 Sanjeevia R, Kumarb G A, Krishnanb B R. Materials Today: Procee-dings, 2020, 8(1), 109.
28 Luo Y H, Guo P, Gao J F, et al. Journal of Cleaner Production, 2022, 379, 134427.
29 Iztok P, Goran Š, Mirjana R, et al. Energies, 2020, 13(14), 3691.
30 Whiffin V S. Microbial CaCO₃ precipitation for the production of biocement. Ph. D. Thesis, Murdoch University, Australia, 2004.
31 Ou Q, Li Y F, Yang Y, et al. Geofluids, 2022, 2022, 1.
32 Ai L Y, Weng C H, Ruan L Y, et al. Mycosystema, 2017, 36(3), 339 (in Chinese).
艾柳英, 翁彩红, 阮玲云, 等. 菌物学报, 2017, 36(3), 339.
33 He Y G, Zhou S, Hu X Y, et al. Journal of South China Agricultural University, 2017, 38(6), 72 (in Chinese).
贺玉广, 周思, 胡晓云, 等. 华南农业大学学报, 2017, 38(6), 72.