基于双硫键的荧光传感器在生物检测及靶向治疗药物输送系统中的应用

doi:10.11896/cldb.18110022

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Abstract Glutathione, which exists in almost every cell of the body, is an important regulatory metabolite in the cell and indispensable for maintaining the normal immune system and biochemical defense system in the human body. Detection of glutathione in real time and quickly is of great significance for disease monitoring and early diagnosis and treatment. Fluorescence sensor based on disulfide bond has received considerable attention due to its rapid response to intracellular glutathione with high sensitivity.
　　Fluorescence sensor based on disulfide bond can not only detect glutathione by fluorescence intensity, but also detect glutathione by ratio fluorescence to avoid interference of background fluorescence in vivo. However, most fluorescence sensors can only achieve the fluorescence detection of thiol content and cannot obtain targeted therapy. At present, in tumor cells with far higher thiol content than normal cells, it is more urgent to realize tumor treatment after diagnosis. In recent years, researchers have done a lot of work in this field and achieved fruitful results. Through ingenious design, fluorescence sensors based on bisulfide bond were designed to combine with anti-tumor drugs within one molecular system to achieve disease diagnosis and treatment.
　　The fluorescent sensor that allows targeted therapeutic release and imaging simultaneously, contains the following elements: ⅰ. fluorescent reporters, ⅱ. cleavable linkers, ⅲ. cancer targeting ligands, ⅳ. masked chemotherapeutic agents. The cleavable disulfide bonds, as the key of designing such fluorescent sensors, can be efficiently cleaved in the high concentration of glutathione in tumor cells to release antitumor drugs (such as camptothecin, adriamycin, cytidine and cisplatin). Excellent fluorophores that have proved attractive for constructing disulfide-based sensors include naphthalimide, coumarin, BODIPY, near-infrared CY7 and so on. The tumor targeting capability may be enhanced by attaching to specific site-localizing entities, such as folate, galactose, vitamin and other local localization groups, which display intrinsic selectivity for can-cer cells over normal cells due to their genetic signatures or because of an overexpression of specific receptors on certain tumor cells. With the rapid development in this field, disulfide-based fluorescent sensors can not only achieve personalized treatment of targeted tumor release drugs, but also monitor the drug release process by fluorescence signal.
　　This review gives a retrospection of the research efforts with respect to the fluorescence sensors based on disulfide bond and targeting drug delivery system, and expounds simply its application in biological imaging and drug delivery, to further deepen the understanding of cell uptake and drug release mechanism. The aim is to promote the further development of molecular design of intracellular fluorescence detection sensors and drug delivery systems.

Key words： disulfide bond targeted drug fluorescent sensor glutathione biological detection

Published: 16 January 2020

CLC number:

O69

About author:: Xiaolin Zhangreceived her Ph.D. degree in applied chemistry from Dalian University of Technology in 2009. After two-year postdoctoral research at Dalian University of Technology, she is currently a full assistant professor in Dalian University. Her research inte-rests are the construction of organic functional mate-rials.

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	ZHANG Xiaolin
	FENG Xiaoting
	ZHAN Shiping
	LU Chunlan
	LI Mingming
	HOU Weimin

Cite this article:

ZHANG Xiaolin,FENG Xiaoting,ZHAN Shiping, et al. The Application of Disulfide-based Fluorescent Sensor in Biological Detection and Targeted Theranostic Drug Delivery[J]. Materials Reports, 2020, 34(5): 5142-5147.

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http://www.mater-rep.com/EN/10.11896/cldb.18110022 OR http://www.mater-rep.com/EN/Y2020/V34/I5/5142

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