水泥基材料中Ca<sup>2+</sup>对矿化微生物的碳酸酐酶产量、活性和矿化的影响

doi:10.11896/cldb.22030269

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Abstract Aiming at an alkali resistant mineralized microorganism suitable for cement-based materials, this work studied the effects of Ca²⁺ concentration change (0—5 mmol/L) in cement-based materials on the enzyme production, carbonic anhydrase activity and the ability to induce CaCO₃ mineralization of mineralized microorganisms. The carbonic anhydrase in bacterial solution with different Ca²⁺ concentration was isolated and purified, and the enzyme yield was determined. The influence mechanism of Ca²⁺ was analyzed by measuring the OD₆₀₀ curve of mineralized microorganism and the enzyme yield of single bacterium;the carbonic anhydrase activity under different Ca²⁺ concentrations was determined by the esterase method, and the functional groups were determined by FTIR. The changes of secondary structure and hydrogen bond were analyzed, and the influence mechanism on the activity was obtained;finally, the mineralization inducing ability of mineralizing microorganisms in different Ca²⁺ concentration solutions was analyzed based on TG, and the crystal form of mineralized products was determined. The test results show that when the concentration of Ca²⁺ was 2.5 mmol/L, the mineralized microorganisms grew and proliferated the fastest and the enzyme yield of a single bacterium was also the highest, so the overall enzyme production was the highest. The activity of anhydrase reached the maximum when the concentration of Ca²⁺ was 5 mmol/L, but Ca²⁺ would destroy the structural of carbonic anhydrase;the mineralizing microorganism has the strongest ability to induce mineralization in 5 mmol/L. After 3 d of mineralization, the conversion rate of Ca(OH)₂ to CaCO₃ reached 82.67%, and the morphology of CaCO₃ crystal was mainly spherical or elliptical.

Key words： mineralized microorganism carbonic anhydrase enzyme yield enzyme activity induce mineralization

Published: 25 April 2024 Online: 2024-04-28

CLC number:

TU502

Fund:National Natural Science Foundation of China (51738003).

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	ZHANG Junjie
	CHEN Yanqiang
	QIAN Chunxiang

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ZHANG Junjie,CHEN Yanqiang,QIAN Chunxiang. Effects of Ca²⁺ in Cement-based Materials on the Yield, Activity and Mineralization of Carbonic Anhydrase of Mineralized Microorganism[J]. Materials Reports, 2024, 38(8): 22030269-6.

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https://www.mater-rep.com/EN/10.11896/cldb.22030269 OR https://www.mater-rep.com/EN/Y2024/V38/I8/22030269

1 De Muynck W, De Belie N, Verstraete W. Ecological Engineering, 2010, 36(2), 118.
2 Qian C X, Ren L F, Xue B, et al. Construction & Building Materials, 2016, 106, 126.
3 Combs J E, Andring J T, Mckenna R. Methods in Enzymology, 2020, 634, 281.
4 Meldrum N U, Roughton F J. The Journal of Physiology, 1933, 80(2), 113.
5 Schneider J, Campion-Alsumard T L. European Journal of Phycology, 1999, 34(4), 417.
6 Bosak T, Greene S E, Newman D K. Geobiology, 2010, 5(2), 119.
7 De Muynck W, Cox K, De Belle N. Construction & Building Materials, 2008, 22(5), 875.
8 Dejong J T, Mortensen B M, Martinez B C, et al. Ecological Enginee-ring, 2010, 36(2), 197.
9 Aloisi G, Gloter A, Krüger M, et al. Geology, 2006, 34(12), 1017.
10 Hammes F, Boon N, de V J, et al. Applied & Environmental Microbiology, 2003, 69(8), 4901.
11 Qian C X, Ren L F. Journal of the Chinese Ceramic Society, 2014, 42(11), 1389.
12 Qian C X, Luo M, Ren L F, et al. Key Engineering Materials, 2014, 629, 494.
13 Zheng T, Qian C. Journal of Materials in Civil Engineering, 2020, 32(11), 04020341.
14 Su Y, Feng J, Jin P, et al. Construction & Building Materials, 2019, 218, 224.
15 Yi H, Qian C X. Science of Advanced Materials, 2020, 12(5), 760.
16 Dejong J T, Fritzges M B, Nüsslein K. Journal of Geotechnical and Geoenvironmental Engineering, 2006, 132(11), 1381.
17 Favre N, Christ M L, Pierre A C. Journal of Molecular Catalysis B Enzymatic, 2009, 60(3-4), 163.
18 Sonia D, Prete D, Vullo V, et al. Journal of Enzyme Inhibition and Medicinal Chemistry, 2014, 29(6), 906.
19 Iverson T M, Alber B E, Kisker C, et al. Biochemistry, 2000, 39(31), 9222.
20 Kikutani S, Nakajima K, Nagasato C, et al. Proceedings of the National Academy of Sciences, 2016, 113(35), 9828.
21 Xu Y, Feng L, Jeffrey P D, et al. Nature, 2008, 452(7183), 56.
22 Sharma T, Kumar A. Environmental Engineering Research, 2021, 26(3), 200191-0.
23 Sundaram S, Thakur I S. Bioresource Technology, 2018, 253, 368.
24 Chen W S. Study on kinetics and morphology of calcium carbonate precipitation catalyzed by bacterial carbonic anhydrase. Master’s Thesis, Huazhong University of Science and Technology, China, 2011(in Chinese).
陈薇珊. 细菌碳酸酐酶催化碳酸钙沉积的动力学和形态学研究. 硕士学位论文, 华中科技大学, 2011.
25 Zhou Z R, Shu Z Y, Li W J, et al. Marine Fisheries, 2017, 39(1), 76(in Chinese).
周子睿, 施志仪, 李文娟, 等. 海洋渔业, 2017, 39(1), 76.
26 Verpoorte J A, Mehta S J, Edsall J T. Journal of Biological Chemistry, 1967, 242(18), 4221.
27 Jackson M, Mantsch H H. Critical Reviews in Biochemistry and Molecular Biology, 1995, 30(2), 95.
28 Carrasquillo M K G. Structure-guided encapsulation of model proteins into biocompatible polymers. Ph. D. Thesis, University of Puerto Rico, USA, 2001.
29 Wang Q W, Yang Y H, Gao H B. Hydrogen bonding problems in orga-nic chemistry, Tianjin University Press, China, 1993, pp.243(in Chinese).
王庆文, 杨玉桓, 高鸿宾. 有机化学中的氢键问题, 天津大学出版社, 1993, pp.243.