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| Improved Magnetofection Efficiency of Polyethylene-modified Fe3O4 Nanoparticles by Exerting a Uniform Magnetic Field |
| XIE Lina1,2,3,4, LUO Cong2, WU Jiamin5, WANG Changxuan1,2,3,4, WU Jun2
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1 Ministry of Education Key Laboratory of Child Development and Disorders, Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, Chongqing 400014;
2 Department of Orthopaedics, Children’s Hospital of ChongqingMedical University, Chongqing 400014;
3 China International Science and Technology Cooperation Base of ChildDevelopment and Critical Disorders, Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, Chongqing 400014;
4 Key Laboratory of Pediatrics, Pediatric Research Institute, Children’s Hospital of ChongqingMedical University, Chongqing 400014;
5 State Key Laboratory of Power Transmission Equipment and SystemSecurity and New Technology, School of Electrical Engineering, Chongqing University, Chongqing 400044 |
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Abstract The effect of uniform magnetic field(MF) on magnetofection was studied for the first time. The uniformity of the unilateral Halbach magnet and the commercialized 96 hole magnet were measured. Polyethyleneimine (PEI) modified ferroferric oxide (Fe3O4) nanoparticles (PEI-Fe3O4) were prepared by chemical coprecipitation. The morphology, composition and the DNA binding ability of PEI-Fe3O4were charaterized by transmission electron microscope (TEM), vibrating sample magnetometer (VSM), atomic force microscopy and agarose gel electrophoresis. The green fluorescent protein (GFP) expression efficiency in human renal epithelial cells (HEK293) under different uniformity of magnetic field was detected with inverted fluorescence microscope and flow cytometry. The phagocytosis of PEI-Fe3O4nanoparticles was observed by TEM. The results showed that the difference of uniformity of the two magnetic fields was about 100 times. The superparamagnetic PEI-Fe3O4 has a good compound ability for plasmid pDNA, and its optimal combination ratio is N/P=0.5. Flow cytometry showed that the transfection efficiency was: PEI-Fe3O4-pDNA+ uniform MF group (77.75%±0.07%)>PEI-Fe3O4-pDNA+ nonuniform MF group (30.65%±0.49%)>PEI-Fe3O4-pDNA without MF group (7.90%±0.56%) (p<0.05). PEI-Fe3O4 magnetic nanoparticles can be effectively consumed by cells and have little effect on cell morphology. It can be concluded that when the magnetic field intensity is certain, higher uniformity of magnetic field contributes to the higher the magnetic transfection efficiency. The combination of the unilateral Halbach magnet and the magnetosfection can be used as a new method to improve the transfection rate, which may potentially be employed in gene therapy.
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Published: 25 April 2018
Online: 2018-05-11
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2018, Vol. 32 
