1 School of Materials Science and Engineering, Southeast University, Nanjing 211189, China 2 Research Center of Green Construction Materials & Carbon Utilization, Southeast University, Nanjing 211189, China 3 Key Laboratory of Microbial Bio-mineralization, China Building Industry, Nanjing 211189, China 4 Jiangsu Key Laboratory of Construction Materials, Southeast University, Nanjing 211189, China
Abstract: Aiming at an alkali resistant mineralized microorganism suitable for cement-based materials, this work studied the effects of Ca2+ concentration change (0—5 mmol/L) in cement-based materials on the enzyme production, carbonic anhydrase activity and the ability to induce CaCO3 mineralization of mineralized microorganisms. The carbonic anhydrase in bacterial solution with different Ca2+ concentration was isolated and purified, and the enzyme yield was determined. The influence mechanism of Ca2+ was analyzed by measuring the OD600 curve of mineralized microorganism and the enzyme yield of single bacterium;the carbonic anhydrase activity under different Ca2+ concentrations was determined by the esterase method, and the functional groups were determined by FTIR. The changes of secondary structure and hydrogen bond were analyzed, and the influence mechanism on the activity was obtained;finally, the mineralization inducing ability of mineralizing microorganisms in different Ca2+ concentration solutions was analyzed based on TG, and the crystal form of mineralized products was determined. The test results show that when the concentration of Ca2+ was 2.5 mmol/L, the mineralized microorganisms grew and proliferated the fastest and the enzyme yield of a single bacterium was also the highest, so the overall enzyme production was the highest. The activity of anhydrase reached the maximum when the concentration of Ca2+ was 5 mmol/L, but Ca2+ would destroy the structural of carbonic anhydrase;the mineralizing microorganism has the strongest ability to induce mineralization in 5 mmol/L. After 3 d of mineralization, the conversion rate of Ca(OH)2 to CaCO3 reached 82.67%, and the morphology of CaCO3 crystal was mainly spherical or elliptical.
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2024, Vol. 38 
